Anand Kumar Katakam
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View article: Deletion of a previously uncharacterized lipoprotein lirL confers resistance to an inhibitor of type II signal peptidase in <i>Acinetobacter baumannii</i>
Deletion of a previously uncharacterized lipoprotein lirL confers resistance to an inhibitor of type II signal peptidase in <i>Acinetobacter baumannii</i> Open
Acinetobacter baumannii is a clinically important, predominantly health care–associated gram-negative bacterium with high rates of emerging resistance worldwide. Given the urgent need for novel antibacterial therapies against A. baumannii …
View article: EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation
EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation Open
Lung development, integrity and repair rely on precise Wnt signaling, which is corrupted in diverse diseases, including cancer. Here, we discover that EHMT2 methyltransferase regulates Wnt signaling in the lung by controlling the transcrip…
View article: Freeze Substitution and Low Temperature Processing of Cryo Protected Tissues into Lowicryl HM23 Resin for Correlated Light and Immunogold Electron Microscopy in Drug Discovery and Safety Assessment Research
Freeze Substitution and Low Temperature Processing of Cryo Protected Tissues into Lowicryl HM23 Resin for Correlated Light and Immunogold Electron Microscopy in Drug Discovery and Safety Assessment Research Open
Freeze Substitution and Low Temperature Processing of Cryo Protected Tissues into Lowicryl HM23 Resin for Correlated Light and Immunogold Electron Microscopy in Drug Discovery and Safety Assessment Research - Volume 28 Issue S1
View article: Author response: EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation
Author response: EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation Open
View article: Inhibition of Escherichia coli Lipoprotein Diacylglyceryl Transferase Is Insensitive to Resistance Caused by Deletion of Braun’s Lipoprotein
Inhibition of Escherichia coli Lipoprotein Diacylglyceryl Transferase Is Insensitive to Resistance Caused by Deletion of Braun’s Lipoprotein Open
As the emerging threat of multidrug-resistant (MDR) bacteria continues to increase, no new classes of antibiotics have been discovered in the last 50 years. While previous attempts to inhibit the lipoprotein biosynthetic (LspA) or transpor…
View article: Author response: Multiplexed proteomics of autophagy-deficient murine macrophages reveals enhanced antimicrobial immunity via the oxidative stress response
Author response: Multiplexed proteomics of autophagy-deficient murine macrophages reveals enhanced antimicrobial immunity via the oxidative stress response Open
View article: Neutrophil serine protease 4 is required for mast cell-dependent vascular leakage
Neutrophil serine protease 4 is required for mast cell-dependent vascular leakage Open
View article: Novel inhibitors of<i>E. coli</i>lipoprotein diacylglyceryl transferase are insensitive to resistance caused by<i>lpp</i>deletion
Novel inhibitors of<i>E. coli</i>lipoprotein diacylglyceryl transferase are insensitive to resistance caused by<i>lpp</i>deletion Open
Lipoprotein diacylglyceryl transferase (Lgt) catalyzes the first step in the biogenesis of Gram-negative bacterial lipoproteins which play crucial roles in bacterial growth and pathogenesis. We demonstrate that Lgt depletion in a clinical …
View article: Proteomics of autophagy deficient macrophages reveals enhanced antimicrobial immunity via the oxidative stress response
Proteomics of autophagy deficient macrophages reveals enhanced antimicrobial immunity via the oxidative stress response Open
Defective autophagy is associated with chronic inflammation. Loss-of-function of the core autophagy gene Atg16l1 increases risk for Crohn’s disease by enhancing innate immunity in macrophages. However, autophagy also mediates clearance of …
View article: Unstable Mechanisms of Resistance to Inhibitors of Escherichia coli Lipoprotein Signal Peptidase
Unstable Mechanisms of Resistance to Inhibitors of Escherichia coli Lipoprotein Signal Peptidase Open
Despite increasing evidence suggesting that antibiotic heteroresistance can lead to treatment failure, the significance of this phenomena in the clinic is not well understood, because many clinical antibiotic susceptibility testing approac…
View article: G9a methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation
G9a methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation Open
Lung development, integrity and repair rely on precise Wnt signaling, which is corrupted in diverse diseases, including cancer. Here, we discover that G9a methyltransferase regulates Wnt signaling in the lung by controlling the transcripti…
View article: Preclinical Safety Assessment of a Highly Selective and Potent Dual Small-Molecule Inhibitor of CBP/P300 in Rats and Dogs
Preclinical Safety Assessment of a Highly Selective and Potent Dual Small-Molecule Inhibitor of CBP/P300 in Rats and Dogs Open
Cyclic adenosine monophosphate-response element (CREB)-binding protein (CBP) and EP300E1A-binding protein (p300) are members of the bromodomain and extraterminal motif (BET) family. These highly homologous proteins have a key role in modul…
View article: Unobstructed Multiscale Imaging of Tissue Sections for Ultrastructural Pathology Analysis by Backscattered Electron Scanning Microscopy
Unobstructed Multiscale Imaging of Tissue Sections for Ultrastructural Pathology Analysis by Backscattered Electron Scanning Microscopy Open
Ultrastructural analysis of healthy, diseased, or experimental tissues is essential in diagnostic and investigative pathology. Evaluation of large tissue areas with suborganelle resolution is challenging because biological structures rangi…
View article: Multi-Scale Imaging of Tissues and Organoid Models by Backscattered Electron Scanning Microscopy after Pre- and Post-Embedding Immuno-Labeling
Multi-Scale Imaging of Tissues and Organoid Models by Backscattered Electron Scanning Microscopy after Pre- and Post-Embedding Immuno-Labeling Open
Ultrastructural investigation of tissues plays an important role in pathology, safety assessment and basic biomedical research.We and others have demonstrated that back-scattered electron scanning electron microscopy (BSE-SEM) can efficien…
View article: Author response: Ubiquilin1 promotes antigen-receptor mediated proliferation by eliminating mislocalized mitochondrial proteins
Author response: Ubiquilin1 promotes antigen-receptor mediated proliferation by eliminating mislocalized mitochondrial proteins Open
View article: Structural insights into lipoprotein N-acylation by <i>Escherichia coli</i> apolipoprotein N-acyltransferase
Structural insights into lipoprotein N-acylation by <i>Escherichia coli</i> apolipoprotein N-acyltransferase Open
Significance Lipoprotein biosynthesis is crucial for Gram-negative bacterial viability and involves the activities of three essential integral membrane proteins embedded in the inner membrane (Lgt, LspA, and Lnt). These enzymes function se…
View article: <i>De Novo</i> Guanine Biosynthesis but Not the Riboswitch-Regulated Purine Salvage Pathway Is Required for Staphylococcus aureus Infection <i>In Vivo</i>
<i>De Novo</i> Guanine Biosynthesis but Not the Riboswitch-Regulated Purine Salvage Pathway Is Required for Staphylococcus aureus Infection <i>In Vivo</i> Open
De novo guanine biosynthesis is an evolutionarily conserved pathway that creates sufficient nucleotides to support DNA replication, transcription, and translation. Bacteria can also salvage nutrients from the environment to supplement the …
View article: Enhancing full-length antibody production by signal peptide engineering
Enhancing full-length antibody production by signal peptide engineering Open
View article: MOESM1 of Enhancing full-length antibody production by signal peptide engineering
MOESM1 of Enhancing full-length antibody production by signal peptide engineering Open
Additional file 1: Table S1. The DNA sequences of the signal peptide TIR variants and the relative TIR strengths.
View article: MOESM2 of Enhancing full-length antibody production by signal peptide engineering
MOESM2 of Enhancing full-length antibody production by signal peptide engineering Open
Additional file 2: Table S2. Strains and plasmids used in this study.
View article: Dendritic cells require NIK for CD40-dependent cross-priming of CD8 <sup>+</sup> T cells
Dendritic cells require NIK for CD40-dependent cross-priming of CD8 <sup>+</sup> T cells Open
Significance The noncanonical NF-κB signaling pathway via the serine kinase NIK (NF-κB–inducing kinase) is essential for normal immune system development and has been implicated in tumor cell survival and growth. Because NIK is under inves…