Anders Barth
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View article: Elucidating the nanoscopic organization and dynamics of the nuclear pore complex
Elucidating the nanoscopic organization and dynamics of the nuclear pore complex Open
Due to its pivotal role as a regulator of nucleocytoplasmic transport, the structure and dynamic gating mechanism of the nuclear pore complex (NPC) is a subject of immense interest. Here, we report key recent advancements discussed at the …
View article: Spatial control of karyopherin binding avidity within NPC mimics revealed by designer FG- Nucleoporins
Spatial control of karyopherin binding avidity within NPC mimics revealed by designer FG- Nucleoporins Open
Nucleocytoplasmic transport occurs via nuclear pore complexes (NPCs), ∼40-60 nm wide pores lined with intrinsically disordered proteins that are rich in Phe-Gly motifs (FG-Nups) that form a selective barrier. Molecules larger than ∼50 kDa …
View article: DNA supercoiling enhances DNA condensation by ParB proteins
DNA supercoiling enhances DNA condensation by ParB proteins Open
The ParABS system plays a critical role in bacterial chromosome segregation. The key component of this system, ParB, loads and spreads along DNA to form a local protein–DNA condensate known as a partition complex. As bacterial chromosomes …
View article: DNA supercoiling enhances DNA condensation by ParB proteins
DNA supercoiling enhances DNA condensation by ParB proteins Open
The ParABS system plays a critical role in bacterial chromosome segregation. The key component of this system, ParB, loads and spreads along DNA to form a local protein-DNA condensate known as a partition complex. As bacterial chromosomes …
View article: Reply to: On the statistical foundation of a recent single molecule FRET benchmark
Reply to: On the statistical foundation of a recent single molecule FRET benchmark Open
In their ‘Matters Arising’ manuscript, Saurabh et al. discuss two issues related to single-molecule Förster resonance energy transfer (smFRET) experiments: the use of the Gaussian noise approximation and spectral crosstalk. Their arguments…
View article: Diameter dependence of transport through nuclear pore complex mimics studied using optical nanopores
Diameter dependence of transport through nuclear pore complex mimics studied using optical nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores
Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Reviewer #1 (Public Review): Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores
Reviewer #1 (Public Review): Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Author Response: Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores
Author Response: Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Reviewer #2 (Public Review): Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores
Reviewer #2 (Public Review): Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells
Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells Open
Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentra…
View article: Publications for MSCA NTON (101029907)
Publications for MSCA NTON (101029907) Open
Repository to deposit publications related to the MSCA Postdoctoral Fellowship NTON (101029907) Klughammer, N., Barth, A., Dekker, M., Fragasso, A., Onck, P. R., & Dekker, C. (2023). Diameter Dependence of Transport through Nuclear Pore Co…
View article: A new twist on PIFE: photoisomerisation-related fluorescence enhancement
A new twist on PIFE: photoisomerisation-related fluorescence enhancement Open
PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as a cyanine, with a protein. This fluorescence enhancement i…
View article: Folding pathway of a discontinuous two-domain protein_2
Folding pathway of a discontinuous two-domain protein_2 Open
This data set is a repository part II for the raw data collected for the preparation of the manuscript "Folding pathway of a discontinuous two-domain protein". Data is given as measured single-molecule TCSPC data as well as the background …
View article: Zero-mode waveguide nanowells for single-molecule detection in living cells
Zero-mode waveguide nanowells for single-molecule detection in living cells Open
Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentra…
View article: Datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells
Datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells Open
Different datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells. The repository contains .zip archives, mostly containing a readme file with additional information. Cell imaging experimen…
View article: Datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells
Datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells Open
Different datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells. The repository contains .zip archives, mostly containing a readme file with additional information. Cell imaging experimen…
View article: Diameter dependence of transport through nuclear pore complex mimics studied using optical nanopores
Diameter dependence of transport through nuclear pore complex mimics studied using optical nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores
Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Folding pathway of a discontinuous two-domain protein_1
Folding pathway of a discontinuous two-domain protein_1 Open
This data set contains all the raw data collected for the preparation of the manuscript "Folding pathway of a discontinuous two-domain protein". Raw data for the main figures Figure 1B-C, Figure 2A, C-D, Figure 4B-C, Figure 5B, C-D and for…
View article: Folding pathway of a discontinuous two-domain protein_1
Folding pathway of a discontinuous two-domain protein_1 Open
This data set contains all the raw data collected for the preparation of the manuscript "Folding pathway of a discontinuous two-domain protein". Raw data for the main figures Figure 1B-C, Figure 2A, C-D, Figure 4B-C, Figure 5B, C-D and for…
View article: Supplementary materials for "Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET"
Supplementary materials for "Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET" Open
Supplementary materials for "Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET", Maslov et al. 1. smFRET data for A2AAR in apo and ligand-bound states in .bur format readable with BurstB…
View article: A new twist on PIFE: photoisomerisation-related fluorescence enhancement
A new twist on PIFE: photoisomerisation-related fluorescence enhancement Open
PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as a cyanine, with a protein. This fluorescence enhancement i…
View article: Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores
Diameter Dependence of Transport through Nuclear Pore Complex Mimics Studied Using Optical Nanopores Open
The nuclear pore complex (NPC) regulates the selective transport of large biomolecules through the nuclear envelope. As a model system for nuclear transport, we construct NPC mimics by functionalizing the pore walls of freestanding palladi…
View article: Folding pathway of a discontinuous two-domain protein_2
Folding pathway of a discontinuous two-domain protein_2 Open
This data set is a repository part II for the raw data collected for the preparation of the manuscript "Folding pathway of a discontinuous two-domain protein". Data is given as measured single-molecule TCSPC data as well as the background …