Andre Bazzone
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View article: Rescue of Epilepsy‐Associated Mutations of the Highly Conserved Glycine Residue 443 in the Human GABA Transporter 1
Rescue of Epilepsy‐Associated Mutations of the Highly Conserved Glycine Residue 443 in the Human GABA Transporter 1 Open
The human γ‐aminobutyric acid (GABA) transporter 1 (hGAT‐1) plays a pivotal role in synaptic neurotransmission by facilitating the clearance of GABA from the synaptic cleft. Pathogenic mutations in the SLC6A1 gene encoding hGAT‐1 have been…
View article: Lysosomal Ion Channels and Transporters: Recent Findings, Therapeutic Potential, and Technical Approaches
Lysosomal Ion Channels and Transporters: Recent Findings, Therapeutic Potential, and Technical Approaches Open
In recent years, there has been a growing interest in lysosomal ion channels and transporters due to their critical role in maintaining lysosomal function and their involvement in a variety of diseases, particularly lysosomal storage disea…
View article: Unraveling pH Regulation of TMEM175, an Endolysosomal Cation Channel With a Role in Parkinson's Disease
Unraveling pH Regulation of TMEM175, an Endolysosomal Cation Channel With a Role in Parkinson's Disease Open
Transmembrane protein 175 (TMEM175) is an endolysosomal cation channel, which has attracted much attention recently from academics and the pharmaceutical industry alike since human mutations in TMEM175 were found to be associated with the …
View article: Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site
Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site Open
The mitochondrial adenosine 5′-diphosphate (ADP)/adenosine 5′-triphosphate (ATP) carrier imports ADP into the mitochondrion and exports ATP to the cell. Here, we demonstrate that 3.3 positive charges are translocated with the negatively ch…
View article: Integration of highly sensitive large-area graphene-based biosensors in an automated sensing platform
Integration of highly sensitive large-area graphene-based biosensors in an automated sensing platform Open
Graphene-based biosensors, featuring exceptional electronic, mechanical, and surface properties, have emerged as frontrunners in advanced sensing technologies. However, to achieve widespread industrial adoption, advancements in the fabrica…
View article: Unveiling the crucial role of betaine: Modulation of GABA homeostasis via SLC6A1 transporter (GAT1)
Unveiling the crucial role of betaine: Modulation of GABA homeostasis via SLC6A1 transporter (GAT1) Open
Betaine is an endogenous osmolyte that exhibits therapeutic potential by mitigating various neurological disorders. However, the underlying cellular and molecular mechanisms responsible for its neuroprotective effects remain puzzling. In t…
View article: Transporter function characterization via continuous-exchange cell-free synthesis and solid supported membrane-based electrophysiology
Transporter function characterization via continuous-exchange cell-free synthesis and solid supported membrane-based electrophysiology Open
Functional characterization of transporters is impeded by the high cost and technical challenges of current transporter assays. Thus, in this work, we developed a new characterization workflow that combines cell-free protein synthesis (CFP…
View article: Unveiling the crucial role of betaine: Modulation of GABA homeostasis via SLC6A1 transporter (GAT1)
Unveiling the crucial role of betaine: Modulation of GABA homeostasis via SLC6A1 transporter (GAT1) Open
Betaine is an endogenous osmolyte that exhibits therapeutic potential by mitigating various neurological disorders. However, the underlying cellular and molecular mechanisms responsible for its neuroprotective effects remain puzzling. In t…
View article: Structural and mechanistic insights into human choline and ethanolamine transport
Structural and mechanistic insights into human choline and ethanolamine transport Open
Human feline leukemia virus subgroup C receptor-related proteins 1 and 2 (FLVCR1 and 2) are members of the major facilitator superfamily 1 . Their dysfunction is linked to several clinical disorders, including PCARP, HSAN, and Fowler syndr…
View article: A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development
A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development Open
The lysosomal cation channel TMEM175 is a Parkinson’s disease-related protein and a promising drug target. Unlike whole-cell automated patch-clamp (APC), lysosomal patch-clamp (LPC) facilitates physiological conditions, but is not yet suit…
View article: A comparative review on the well-studied GAT1 and the understudied BGT-1 in the brain
A comparative review on the well-studied GAT1 and the understudied BGT-1 in the brain Open
γ-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system (CNS). Its homeostasis is maintained by neuronal and glial GABA transporters (GATs). The four GATs identified in humans are GAT1 (SLC6A1), …
View article: Functional characterization of SGLT1 using SSM-based electrophysiology: Kinetics of sugar binding and translocation
Functional characterization of SGLT1 using SSM-based electrophysiology: Kinetics of sugar binding and translocation Open
Beside the ongoing efforts to determine structural information, detailed functional studies on transporters are essential to entirely understand the underlying transport mechanisms. We recently found that solid supported membrane-based ele…
View article: Structure, mechanism and lipid-mediated remodeling of the mammalian Na+/H+ exchanger NHA2
Structure, mechanism and lipid-mediated remodeling of the mammalian Na+/H+ exchanger NHA2 Open
The Na + /H + exchanger SLC9B2, also known as NHA2, correlates with the long-sought-after Na + /Li + exchanger linked to the pathogenesis of diabetes mellitus and essential hypertension in humans. Despite the functional importance of NHA2,…
View article: Structure and lipid-mediated remodelling mechanism of the Na<sup>+</sup>/H<sup>+</sup>exchanger NHA2
Structure and lipid-mediated remodelling mechanism of the Na<sup>+</sup>/H<sup>+</sup>exchanger NHA2 Open
Na + /H + exchangers catalyse an ion-exchange activity that is carried out in most, if not all cells. SLC9B2, also known as NHA2, correlates with the long-sought after sodium/lithium (Na + /Li + ) exchanger linked to the pathogenesis of di…
View article: pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases
pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases Open
Bacterial sugar symporters in the Major Facilitator Superfamily (MFS) use the H+ (and in a few cases Na+) electrochemical gradients to achieve active transport of sugar into the cell. Because a number of structures of MFS sugar symporters …