André Nadler
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View article: Bifunctional Probes Reveal the Rules of Intracellular Ether Lipid Transport
Bifunctional Probes Reveal the Rules of Intracellular Ether Lipid Transport Open
Ether glycerophospholipids bear a long chain alcohol attached via an alkyl or vinyl ether bond at the sn1 position of the glycerol backbone. Ether lipids play a significant role in physiology and human health. However, their cellular funct…
View article: The Lipid Interactome: An interactive and open access platform for exploring cellular lipid-protein interactomes.
The Lipid Interactome: An interactive and open access platform for exploring cellular lipid-protein interactomes. Open
Lipid-protein interactions play essential roles in cellular signaling and membrane dynamics, yet their systematic characterization has long been hindered by the inherent biochemical properties of lipids. Recent advances in functionalized l…
View article: GEQO biosensors for absolute analyte quantification in single cells
GEQO biosensors for absolute analyte quantification in single cells Open
Genetically encoded fluorescent biosensors are widely used to monitor small molecule and ion levels in living cells. Quantitative FRET and FLIM sensors and highly sensitive intensiometric sensors have been developed for many analytes. Notw…
View article: Disruption of Plasma Membrane Lipid Asymmetry Alters Cellular Energetics
Disruption of Plasma Membrane Lipid Asymmetry Alters Cellular Energetics Open
Biological membranes often feature an unequal and actively maintained concentration gradients of lipids between bilayer leaflets, termed lipid asymmetry. 1–3 Lipid asymmetry has been linked to many cellular processes 4,5 , but the mechanis…
View article: Quantitative imaging of species-specific lipid transport in mammalian cells - endocytosis inhibitors
Quantitative imaging of species-specific lipid transport in mammalian cells - endocytosis inhibitors Open
To test the predominant role of the non-vesicular route for retrograde transport in U-2 OS cells, we inhibited vesicular trafficking using Brefeldin A, which blocks COPI coat formation at Golgi membranes and Wortmannin, which broadly affec…
View article: Visualizing sub-organellar lipid distribution using correlative light and electron microscopy
Visualizing sub-organellar lipid distribution using correlative light and electron microscopy Open
Lipids and proteins compartmentalize biological membranes into nanoscale domains which are crucial for signaling, intracellular trafficking and many other cellular processes. Studying nanodomain function requires the ability to measure pro…
View article: A Photo‐Caged Cross‐Linker for Identifying Protein‐Protein Interactions
A Photo‐Caged Cross‐Linker for Identifying Protein‐Protein Interactions Open
Cross‐linking mass spectrometry (XL–MS) has seen significant improvements which have enhanced its utility for studying protein‐protein interactions (PPIs), primarily due to the emergence of novel crosslinkers and the development of streaml…
View article: Bifunctional probes reveal the rules of intracellular ether lipid transport
Bifunctional probes reveal the rules of intracellular ether lipid transport Open
Ether glycerophospholipids bear a long chain alcohol attached via an alkyl or vinyl ether bond at the sn1 position of the glycerol backbone. Emerging evidence suggests that ether lipids play a significant role in physiology and human healt…
View article: Quantitative imaging of species-specific lipid transport in mammalian cells
Quantitative imaging of species-specific lipid transport in mammalian cells Open
Eukaryotic cells produce over 1000 different lipid species which tune organelle membrane properties, control signalling and store energy 1,2 . How lipid species are selectively sorted between organelles to maintain specific membrane identi…
View article: Abstract 2353 Lipid flux: Tracking lipids through physical & chemical space
Abstract 2353 Lipid flux: Tracking lipids through physical & chemical space Open
Eukaryotic cells produce over 1000 different lipid species which tune organelle membrane properties, control signalling and store energy. Organelle-specific lipid distributions are maintained by local metabolism and transport of lipids via…
View article: Bifunctional Monosaccharides Preferentially Localize to Nuclear Subcompartments
Bifunctional Monosaccharides Preferentially Localize to Nuclear Subcompartments Open
Recent progress in glycan research has been driven by widespread implementations of metabolic oligosaccharide engineering. Complementing existing approaches, we here introduce bifunctional, UV-crosslinkable and clickable N-acetylglucosamin…
View article: Ectocytosis renders T cell receptor signaling self-limiting at the immune synapse
Ectocytosis renders T cell receptor signaling self-limiting at the immune synapse Open
Cytotoxic T lymphocytes (CTLs) kill virus-infected and cancer cells through T cell receptor (TCR) recognition. How CTLs terminate signaling and disengage to allow serial killing has remained a mystery. TCR activation triggers membrane spec…
View article: The Road to Quantitative Lipid Biochemistry in Living Cells
The Road to Quantitative Lipid Biochemistry in Living Cells Open
Traditional cell biological techniques are not readily suitable for studying lipid signaling events because genetic perturbations are much slower than the interconversion of lipids in complex metabolic networks. For this reason, novel chem…
View article: Quantifying single cell lipid signaling kinetics after photo-stimulation
Quantifying single cell lipid signaling kinetics after photo-stimulation Open
Studying the role of molecularly distinct lipid species in cell signaling remains challenging due to a scarcity of methods for performing quantitative lipid biochemistry in living cells. We have recently used lipid uncaging to quantify lip…
View article: Influence of different adhesion strategies on glass fiber post retention
Influence of different adhesion strategies on glass fiber post retention Open
Self-etching (G4) and self-adhesive resin (G5) cements, showed similar BS results of immediate bonding in the cementation of GFP compared to conventional resin cements (G1, G2, G3). Key words:Dental Cements, Dentin-Bonding Agents, Post and…
View article: Caged lipid probes for controlling lipid levels on subcellular scales
Caged lipid probes for controlling lipid levels on subcellular scales Open
Lipids exert their cellular functions in individual organelles, in some cases on the scale of even smaller, specialized membrane domains. Thus, the experimental capacity to precisely manipulate lipid levels at the subcellular level is cruc…
View article: Hedgehog signaling can enhance glycolytic <scp>ATP</scp> production in the <i>Drosophila</i> wing disc
Hedgehog signaling can enhance glycolytic <span>ATP</span> production in the <i>Drosophila</i> wing disc Open
Adenosine triphosphate (ATP) production and utilization is critically important for animal development. How these processes are regulated in space and time during tissue growth remains largely unclear. We used a FRET-based sensor to dynami…
View article: Hedgehog signaling can enhance glycolytic ATP production in the <i>Drosophila</i> wing disc
Hedgehog signaling can enhance glycolytic ATP production in the <i>Drosophila</i> wing disc Open
Energy production and utilization is critically important for animal development and growth. How it is regulated in space and time during tissue growth remains largely unclear. Toward this end, we used a FRET-based adenosine triphosphate (…
View article: Messages across time and space
Messages across time and space Open
Compartmentalized oscillations of second messengers affect global cellular signaling.
View article: Glycolysis regulates Hedgehog signalling via the plasma membrane potential
Glycolysis regulates Hedgehog signalling via the plasma membrane potential Open
Changes in cell metabolism and plasma membrane potential have been linked to shifts between tissue growth and differentiation, and to developmental patterning. How such changes mediate these effects is poorly understood. Here, we use the d…
View article: Live-cell lipid biochemistry reveals a role of diacylglycerol side-chain composition for cellular lipid dynamics and protein affinities
Live-cell lipid biochemistry reveals a role of diacylglycerol side-chain composition for cellular lipid dynamics and protein affinities Open
Every cell produces thousands of distinct lipid species, but insight into how lipid chemical diversity contributes to biological signaling is lacking, particularly because of a scarcity of methods for quantitatively studying lipid function…
View article: A Coumarin Triflate Reagent Enables One‐Step Synthesis of Photo‐Caged Lipid Metabolites for Studying Cell Signaling
A Coumarin Triflate Reagent Enables One‐Step Synthesis of Photo‐Caged Lipid Metabolites for Studying Cell Signaling Open
Photorelease of caged compounds is among the most powerful experimental approaches for studying cellular functions on fast timescales. However, its full potential has yet to be exploited, as the number of caged small molecules available fo…
View article: Quantitative Fragmentation Model for Bottom-Up Shotgun Lipidomics
Quantitative Fragmentation Model for Bottom-Up Shotgun Lipidomics Open
Quantitative bottom-up shotgun lipidomics relies on molecular species-specific "signature" fragments consistently detectable in tandem mass spectra of analytes and standards. Molecular species of glycerophospholipids are typically quantifi…
View article: Small molecule modulation of a redox-sensitive stress granule protein dissolves stress granules with beneficial outcomes for familial amyotrophic lateral sclerosis models
Small molecule modulation of a redox-sensitive stress granule protein dissolves stress granules with beneficial outcomes for familial amyotrophic lateral sclerosis models Open
Neurodegeneràve diseases such as amyotrophic lateral sclerosis (ALS) are oten associated with mutàons in proteins that are associated with stress granules. Stress granules are condensates formed by liquid-liquid phase separàon which, when …