Andrea Belluati
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View article: Characterization and Optimization of Vesicle Properties in bioPISA: from Size Distribution to Post‐Assembly Loading
Characterization and Optimization of Vesicle Properties in bioPISA: from Size Distribution to Post‐Assembly Loading Open
This study investigates the formation and properties of vesicles produced via biocatalytic Polymerization‐Induced Self‐Assembly (bioPISA) as artificial cells. Methods for achieving size uniformity, including gentle centrifugation and sucro…
View article: Enzymatic Reaction Network‐Driven Polymerization‐Induced Transient Coacervation
Enzymatic Reaction Network‐Driven Polymerization‐Induced Transient Coacervation Open
A living cell has a highly complex microenvironment whereas numerous enzyme‐driven processes are active at once. These procedures are incredibly accurate and efficient, although comparable control has not yet been established in vitro. Her…
View article: Characterization and Optimization of Vesicle Properties in bioPISA: From Size Distribution to Post-Assembly Loading
Characterization and Optimization of Vesicle Properties in bioPISA: From Size Distribution to Post-Assembly Loading Open
This study investigates the formation and properties of vesicles produced via biocatalytic Polymerization-Induced Self-Assembly (bioPISA) as artificial cells. We explore methods for achieving size uniformity, including gentle centrifugatio…
View article: BioATRP synthesized ATP-fueled Transient Coacervation
BioATRP synthesized ATP-fueled Transient Coacervation Open
A living cell has a highly complex microenvironment whereas numerous enzyme-driven processes are active at once. These procedures are incredibly accurate and efficient, although comparable control has not yet been established in vitro. Her…
View article: Microfluidically Produced Microcapsules with Amphiphilic Polymer Conetwork Shells
Microfluidically Produced Microcapsules with Amphiphilic Polymer Conetwork Shells Open
Microcapsules with an aqueous core can be conveniently prepared by water‐in‐oil‐in‐water double emulsion microfluidics. However, conventional shell materials are based on hydrophobic polymers or colloidal particles. Thus, these microcapsul…
View article: Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations
Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations Open
This repository contains numerical and graphical data for the submission Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations
View article: Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations
Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations Open
This repository contains numerical and graphical data for the submission Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations
View article: Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations
Self-Decorating Cells Via Surface-Initiated Enzymatic Controlled Radical Polymerizations Open
Innovatively utilizing surface-displayed horseradish peroxidase, this paper explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioA…
View article: Microfluidically produced microcapsules with amphiphilic polymer conetwork shells
Microfluidically produced microcapsules with amphiphilic polymer conetwork shells Open
Microcapsules with aqueous core can be conveniently prepared by water-in-oil-in-water double emulsion microfluidics. However, conventional shell materials are either based on polymers or monomers that are soluble in the oil phase, or based…
View article: An Outer Membrane‐Inspired Polymer Coating Protects and Endows <i>Escherichia coli</i> with Novel Functionalities
An Outer Membrane‐Inspired Polymer Coating Protects and Endows <i>Escherichia coli</i> with Novel Functionalities Open
A bio‐inspired membrane made of Pluronic L‐121 is produced around Escherichia coli thanks to the simple co‐extrusion of bacteria and polymer vesicles. The block copolymer‐coated bacteria can withstand various harsh shocks, for example, tem…
View article: Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly
Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly Open
Artificial cells are biomimetic microstructures that mimic functions of natural cells and find application, e.g., as microreactors, as building blocks for molecular systems engineering, and to host synthetic biology pathways. Here, we repo…
View article: Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly
Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly Open
Artificial cells are biomimetic microstructures that mimic functions of natural cells and find application, e.g., as microreactors, as building blocks for molecular systems engineering, and to host synthetic biology pathways. Here, we repo…
View article: Self-decorating cells <i>via</i> surface-initiated enzymatic controlled radical polymerization
Self-decorating cells <i>via</i> surface-initiated enzymatic controlled radical polymerization Open
Through the use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT and bioATRP to prompt polymer synthesis on the surface of S. cerevisiae cells, with bioATRP outperforming bioRAFT polym…
View article: An outer membrane-inspired polymer coating protects and endows E. coli with novel functionalities
An outer membrane-inspired polymer coating protects and endows E. coli with novel functionalities Open
A bio-inspired membrane made of Pluronic® L-121 is produced around E. coli thanks to the simple co-extrusion of bacteria and polymer vesicles. The block copolymer-coated bacteria can withstand a variety of harsh shocks, e.g. temperature, p…
View article: Clustering of Catalytic Nanocompartments for Enhancing an Extracellular Non-Native Cascade Reaction
Clustering of Catalytic Nanocompartments for Enhancing an Extracellular Non-Native Cascade Reaction Open
Data underlying the figures in the publication “Clustering of Catalytic Nanocompartments for Enhancing an Extracellular Non-Native Cascade Reaction”, published in Chemical Science 2021. Table of contents: 1. Figure 2; Zip. archive containi…
View article: Clustering of Catalytic Nanocompartments for Enhancing an Extracellular Non-Native Cascade Reaction
Clustering of Catalytic Nanocompartments for Enhancing an Extracellular Non-Native Cascade Reaction Open
Data underlying the figures in the publication “Clustering of Catalytic Nanocompartments for Enhancing an Extracellular Non-Native Cascade Reaction”, published in Chemical Science 2021. Table of contents: 1. Figure 2; Zip. archive containi…
View article: Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade
Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade Open
Data underlying the figures in the publication “Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade”, published in ACS Nano, 2020, 14, 9, 12101–12112. https://pubs.acs.…
View article: Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade
Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade Open
Data underlying the figures in the publication “Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade”, published in ACS Nano, 2020, 14, 9, 12101–12112. https://pubs.acs.…
View article: DNA-Directed Arrangement of Soft Synthetic Compartments and Their Behavior In Vitro and In Vivo
DNA-Directed Arrangement of Soft Synthetic Compartments and Their Behavior In Vitro and In Vivo Open
Data underlying the figures in the publication “DNA-Directed Arrangement of Soft Synthetic Compartments and Their Behavior in Vitro and in Vivo”, published in Nanoscale, 2020, 12, 9786–9799. https://pubs.rsc.org/en/content/articlelanding/2…
View article: DNA-Directed Arrangement of Soft Synthetic Compartments and Their Behavior In Vitro and In Vivo
DNA-Directed Arrangement of Soft Synthetic Compartments and Their Behavior In Vitro and In Vivo Open
Data underlying the figures in the publication “DNA-Directed Arrangement of Soft Synthetic Compartments and Their Behavior in Vitro and in Vivo”, published in Nanoscale, 2020, 12, 9786–9799. https://pubs.rsc.org/en/content/articlelanding/2…
View article: Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments
Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments Open
Data underlying the figures in the publication “Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments”, published in Adv. Mater., 2020, 32, 2004804. https://onlinelibrary.wiley.com/do…
View article: Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments
Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments Open
Data underlying the figures in the publication “Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments”, published in Adv. Mater., 2020, 32, 2004804. https://onlinelibrary.wiley.com/do…
View article: Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity
Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity Open
Data underlying the figures in the publication “Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity”, published in J. Phys. Chem. B., 2020, 124, 22, 4454–4465. https://…
View article: Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity
Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity Open
Data underlying the figures in the publication “Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity”, published in J. Phys. Chem. B., 2020, 124, 22, 4454–4465. https://…
View article: Clustering of catalytic nanocompartments for enhancing an extracellular non-native cascade reaction
Clustering of catalytic nanocompartments for enhancing an extracellular non-native cascade reaction Open
Compartmentalization is fundamental in nature, where the spatial segregation of biochemical reactions within and between cells ensures optimal conditions for the regulation of cascade reactions.
View article: Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell‐Sized Compartments
Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell‐Sized Compartments Open
Cells rely upon producing enzymes at precise rates and stoichiometry for maximizing functionalities. The reasons for this optimal control are unknown, primarily because of the interconnectivity of the enzymatic cascade effects within multi…
View article: Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade
Bioactive Catalytic Nanocompartments Integrated into Cell Physiology and Their Amplification of a Native Signaling Cascade Open
Bioactive nanomaterials have the potential to overcome the limitations of classical pharmacological approaches by taking advantage of native pathways to influence cell behavior, interacting with them and eliciting responses. Herein, we pro…
View article: Multicompartment Polymer Vesicles with Artificial Organelles for Signal‐Triggered Cascade Reactions Including Cytoskeleton Formation
Multicompartment Polymer Vesicles with Artificial Organelles for Signal‐Triggered Cascade Reactions Including Cytoskeleton Formation Open
Organelles, i.e., internal subcompartments of cells, are fundamental to spatially separate cellular processes, while controlled intercompartment communication is essential for signal transduction. Furthermore, dynamic remodeling of the cyt…