Andrea Schenkmayerová
YOU?
Author Swipe
View article: Kinetic mechanism of Renilla luciferase guides induced-fit engineering for improved bioluminescence
Kinetic mechanism of Renilla luciferase guides induced-fit engineering for improved bioluminescence Open
Renilla luciferase (RLuc) remains one of the most popular bioluminescence reporters, but its molecular principle has yet to be fully understood. Here, we reveal a detailed kinetic mechanism of the RLuc catalytic cycle which uncovers multip…
View article: Multimeric structure of a subfamily <scp>III</scp> haloalkane dehalogenase‐like enzyme solved by combination of <scp>cryo‐EM</scp> and x‐ray crystallography
Multimeric structure of a subfamily <span>III</span> haloalkane dehalogenase‐like enzyme solved by combination of <span>cryo‐EM</span> and x‐ray crystallography Open
Haloalkane dehalogenase (HLD) enzymes employ an S N 2 nucleophilic substitution mechanism to erase halogen substituents in diverse organohalogen compounds. Subfamily I and II HLDs are well‐characterized enzymes, but the mode and purpose of…
View article: LoopGrafter: a web tool for transplanting dynamical loops for protein engineering
LoopGrafter: a web tool for transplanting dynamical loops for protein engineering Open
The transplantation of loops between structurally related proteins is a compelling method to improve the activity, specificity and stability of enzymes. However, despite the interest of loop regions in protein engineering, the available me…
View article: A catalytic mechanism for <i>Renilla</i>-type bioluminescence
A catalytic mechanism for <i>Renilla</i>-type bioluminescence Open
The widely used coelenterazine-powered Renilla luciferase was discovered over 40 years ago but the oxidative mechanism by which it generates blue photons remains unclear. Here we decipher Renilla -type bioluminescence through crystallograp…
View article: Catalytic mechanism for Renilla-type luciferases
Catalytic mechanism for Renilla-type luciferases Open
The widely used coelenterazine-powered Renilla luciferase was discovered over 40 years ago but the oxidative mechanism by which it generates blue photons remains unclear. Here we decipher Renilla-type catalysis through crystallographic, sp…
View article: Catalytic mechanism for Renilla-type luciferases
Catalytic mechanism for Renilla-type luciferases Open
The widely used coelenterazine-powered Renilla luciferase was discovered over 40 years ago but the oxidative mechanism by which it generates blue photons remains unclear. Here we decipher Renilla-type catalysis through crystallographic, sp…
View article: Increased Occurrence of <i>Treponema spp</i>. and Double-species Infections in Patients with Alzheimer’s Disease
Increased Occurrence of <i>Treponema spp</i>. and Double-species Infections in Patients with Alzheimer’s Disease Open
Objective Although the link between microbial infections and Alzheimer’s disease (AD) has been demonstrated in multiple studies, the involvement of pathogens in the development of AD remains unclear. Therefore, this theory beckons further …
View article: Engineering the protein dynamics of an ancestral luciferase
Engineering the protein dynamics of an ancestral luciferase Open
View article: Engineering Protein Dynamics of Ancestral Luciferase
Engineering Protein Dynamics of Ancestral Luciferase Open
Insertion-deletion mutations are sources of major functional innovations in naturally evolved proteins, but directed evolution methods rely primarily on substitutions. Here, we report a powerful strategy for engineering backbone dynamics b…
View article: Engineering Protein Dynamics of Ancestral Luciferase
Engineering Protein Dynamics of Ancestral Luciferase Open
Insertion-deletion mutations are sources of major functional innovations in naturally evolved proteins, but directed evolution methods rely primarily on substitutions. Here, we report a powerful strategy for engineering backbone dynamics b…