Andreas Bechthold
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View article: Heme dependent activity of the Streptomyces c-di-GMP-metabolizing enzyme CdgA
Heme dependent activity of the Streptomyces c-di-GMP-metabolizing enzyme CdgA Open
Streptomyces species are vital for producing natural products like antibiotics, with c-di-GMP playing a key role in regulating processes such as differentiation. C-di-GMP metabolism is controlled by diguanylate cyclases (DGCs) and phosphod…
View article: Olikomycin A–A Novel Calcium‐Dependent Lipopeptide with Antibiotic Activity Against Multidrug‐Resistant Bacteria
Olikomycin A–A Novel Calcium‐Dependent Lipopeptide with Antibiotic Activity Against Multidrug‐Resistant Bacteria Open
Research into new antibiotics is becoming increasingly important as antibiotic resistance increases worldwide. The genus Streptomyces in particular is able to produce a wide range of antimicrobial products due to the large number of biosyn…
View article: Genomic modifications for enhanced antibiotic production in rifamycin derivative-producing Amycolatopsis mediterranei S699 strains: focusing on rifQ and rifO genes
Genomic modifications for enhanced antibiotic production in rifamycin derivative-producing Amycolatopsis mediterranei S699 strains: focusing on rifQ and rifO genes Open
Rifamycin and its derivatives are natural products that belong to the class of antibiotic-active polyketides and have significant therapeutic relevance within the therapy scheme of tuberculosis, a worldwide infectious disease caused by Myc…
View article: Analysis of the cryptic biosynthetic gene cluster encoding the RiPP curacozole reveals a phenylalanine-specific peptide hydroxylase
Analysis of the cryptic biosynthetic gene cluster encoding the RiPP curacozole reveals a phenylalanine-specific peptide hydroxylase Open
The noncanonical biosynthesis of curacozole by Streptomyces curacoi is shown to be dependent on the bldA -encoded Leu-tRNA UUA . The minimal biosynthetic gene cluster is defined, and the functions of key biosynthetic enzymes are establishe…
View article: Identification of RimR2 as a positive pathway-specific regulator of rimocidin biosynthesis in Streptomyces rimosus M527
Identification of RimR2 as a positive pathway-specific regulator of rimocidin biosynthesis in Streptomyces rimosus M527 Open
Background Streoptomyces rimosus M527 is a producer of the polyene macrolide rimocidin which shows activity against various plant pathogenic fungi. Notably, the regulatory mechanisms underlying rimocidin biosynthesis are yet to be elucidat…
View article: Improvement of rimocidin production in <i>Streptomyces rimosus</i> M527 by reporter-guided mutation selection
Improvement of rimocidin production in <i>Streptomyces rimosus</i> M527 by reporter-guided mutation selection Open
In this study, we employed a reporter-guided mutation selection (RGMS) strategy to improve the rimocidin production of Streptomyces rimosus M527, which is based on a single-reporter plasmid pAN and atmospheric and room temperature plasma (…
View article: Intratumoral delivered novel circular mRNA encoding cytokines for immune modulation and cancer therapy
Intratumoral delivered novel circular mRNA encoding cytokines for immune modulation and cancer therapy Open
The success of the two mRNA vaccines developed by Moderna and BioNTech during the COVID-19 pandemic increased research interest into the application of mRNA technologies. Compared with the canonical linear mRNA used in these vaccines, circ…
View article: Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity Open
Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and b…
View article: Cover Feature: Identification of Genes Essential for Sulfamate and Fluorine Incorporation During Nucleocidin Biosynthesis (ChemBioChem 15/2022)
Cover Feature: Identification of Genes Essential for Sulfamate and Fluorine Incorporation During Nucleocidin Biosynthesis (ChemBioChem 15/2022) Open
Nucleocidin is a rare example of naturally occurring nucleoside containing fluorine and sulfamate substituents. Identification of Streptomyces strains that are better producers of nucleocidin has facilitated the trek towards elucidating th…
View article: In vitro and in silico Studies Reveal Bacillus cereus AA-18 as a Potential Candidate for Bioremediation of Mercury-Contaminated Wastewater
In vitro and in silico Studies Reveal Bacillus cereus AA-18 as a Potential Candidate for Bioremediation of Mercury-Contaminated Wastewater Open
Mercury (Hg) pollution is a worldwide problem and increasing day by day due to natural and anthropogenic sources. In this study, mercury-resistant (Hg R ) bacterial isolates were isolated from industrial wastewater of Ittehad Chemicals Ltd…
View article: C-di-GMP and Its Role in Regulation of Natural Products Production
C-di-GMP and Its Role in Regulation of Natural Products Production Open
Among clinical and industrial important microorganisms Streptomyces are the largest source of natural products. But only a fraction of all Biosynthetic Gene Clusters in their genome is active under laboratory conditions.
View article: Identification of Genes Essential for Sulfamate and Fluorine Incorporation During Nucleocidin Biosynthesis
Identification of Genes Essential for Sulfamate and Fluorine Incorporation During Nucleocidin Biosynthesis Open
Nucleocidin is an adenosine derivative containing 4’‐fluoro and 5’‐ O ‐sulfamoyl substituents. In this study, nucleocidin biosynthesis is examined in two newly discovered producers, Streptomyces virens B‐24331 and Streptomyces aureorectus …
View article: Genetic Engineering of Streptomyces ghanaensis ATCC14672 for Improved Production of Moenomycins
Genetic Engineering of Streptomyces ghanaensis ATCC14672 for Improved Production of Moenomycins Open
Streptomycetes are soil-dwelling multicellular microorganisms famous for their unprecedented ability to synthesize numerous bioactive natural products (NPs). In addition to their rich arsenal of secondary metabolites, Streptomyces are char…
View article: Intratumoral Delivered Novel Circular mRNA Encoding Cytokines for Immune Modulation and Cancer Therapy
Intratumoral Delivered Novel Circular mRNA Encoding Cytokines for Immune Modulation and Cancer Therapy Open
The application of mRNA as a novel kind of vaccine has been proved recently, due to the emergence use authorization (EUA) by FDA for the two COVID-19 mRNA vaccines developed by Moderna and BioNTech. Both of the two vaccines are based on ca…
View article: Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628
Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628 Open
Streptomyces are famous for their ability to synthesize a large number of bioactive compounds as secondary metabolites containing antibiotics, enzyme inhibitors, and other small molecules with potential physiological activity (Niu et al., …
View article: Draft Genome Sequence of Marine Actinobacterium Streptomyces spinoverrucosus SNB-032
Draft Genome Sequence of Marine Actinobacterium Streptomyces spinoverrucosus SNB-032 Open
Actinobacteria represent a large source of diverse bioactive compounds of medical and economic importance. Here, we report the 8.8-Mb draft genome of the marine bacterium Streptomyces spinoverrucosus SNB-032. Bioinformatic sequence analysi…
View article: Regulatory Control of Rishirilide(s) Biosynthesis in Streptomyces bottropensis
Regulatory Control of Rishirilide(s) Biosynthesis in Streptomyces bottropensis Open
Streptomycetes are well-known producers of numerous bioactive secondary metabolites widely used in medicine, agriculture, and veterinary. Usually, their genomes encode 20–30 clusters for the biosynthesis of natural products. Generally, the…
View article: Identification and Characterization of Four c-di-GMP-Metabolizing Enzymes from Streptomyces ghanaensis ATCC14672 Involved in the Regulation of Morphogenesis and Moenomycin A Biosynthesis
Identification and Characterization of Four c-di-GMP-Metabolizing Enzymes from Streptomyces ghanaensis ATCC14672 Involved in the Regulation of Morphogenesis and Moenomycin A Biosynthesis Open
Diguanylate cyclases (DGCs) and phosphodiesterases (PDEs) are essential enzymes deputed to maintain the intracellular homeostasis of the second messenger cyclic dimeric (3′→5′) GMP (c-di-GMP). Recently, c-di-GMP has emerged as a crucial mo…
View article: Draft Genome Sequence of <i>Streptomyces</i> sp. Strain BR123, Endowed with Broad-Spectrum Antimicrobial Potential
Draft Genome Sequence of <i>Streptomyces</i> sp. Strain BR123, Endowed with Broad-Spectrum Antimicrobial Potential Open
The genome of Streptomyces sp. strain BR123, isolated from rhizospheric soil that exhibited promising antimicrobial properties, was sequenced and assembled. Here, we report an 8,157,040-bp genome sequence with a G+C content of 72.63%. This…
View article: Cloning and Overexpression of the Toy Cluster for Titer Improvement of Toyocamycin in Streptomyces diastatochromogenes
Cloning and Overexpression of the Toy Cluster for Titer Improvement of Toyocamycin in Streptomyces diastatochromogenes Open
The nucleoside antibiotic toyocamycin (TM) is a potential fungicide that can control plant diseases, and it has become an attractive target for research. Streptomyces diastatochromogenes 1628, a TM-producing strain, was isolated by our lab…
View article: Identification and Characterization of a Novel N- and O-Glycosyltransferase from Saccharopolyspora erythraea
Identification and Characterization of a Novel N- and O-Glycosyltransferase from Saccharopolyspora erythraea Open
Glycosyltransferases are important enzymes which are often used as tools to generate novel natural products. In this study, we describe the identification and characterization of an inverting N- and O-glycosyltransferase from Saccharopolys…
View article: Cyclic di-GMP cyclase SSFG_02181 from Streptomyces ghanaensis ATCC14672 regulates antibiotic biosynthesis and morphological differentiation in streptomycetes
Cyclic di-GMP cyclase SSFG_02181 from Streptomyces ghanaensis ATCC14672 regulates antibiotic biosynthesis and morphological differentiation in streptomycetes Open
Streptomycetes are filamentous bacteria famous for their ability to produce a vast majority of clinically important secondary metabolites. Both complex morphogenesis and onset of antibiotic biosynthesis are tightly linked in streptomycetes…
View article: Role of Two Exceptional <i>trans</i> Adenylation Domains and MbtH‐like Proteins in the Biosynthesis of the Nonribosomal Peptide WS9324A from <i>Streptomyces calvus</i> ATCC 13382
Role of Two Exceptional <i>trans</i> Adenylation Domains and MbtH‐like Proteins in the Biosynthesis of the Nonribosomal Peptide WS9324A from <i>Streptomyces calvus</i> ATCC 13382 Open
Nonribosomal peptide synthetases (NRPS) are organized in a modular arrangement. Usually, the modular order corresponds to the assembly of the amino acids in the respective peptide, following the collinearity rule. The WS9326A biosynthetic …
View article: Early Steps in the Biosynthetic Pathway of Rishirilide B
Early Steps in the Biosynthetic Pathway of Rishirilide B Open
The biological active compound rishirilide B is produced by Streptomyces bottropensis. The cosmid cos4 contains the complete rishirilide B biosynthesis gene cluster. Its heterologous expression in the host Streptomyces albus J1074 led to t…
View article: Identification of a gene from Streptomyces rimosus M527 negatively affecting antibiotic biosynthesis and morphological differentiation
Identification of a gene from Streptomyces rimosus M527 negatively affecting antibiotic biosynthesis and morphological differentiation Open
Background: The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, was found to be highly effective against a broad range of fungal plant pathogens. Current understanding of the regulatory mechanism of rimocidin biosynthes…