Andreas Diepold
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View article: Activation of the Yersinia type III secretion system induces large-scale spatial reorganization of chromosomal and virulence plasmid DNA
Activation of the Yersinia type III secretion system induces large-scale spatial reorganization of chromosomal and virulence plasmid DNA Open
View article: Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system Open
Major bacterial pathogens manipulate eukaryotic target cells by injecting effector proteins through type III secretion systems (T3SS). Recent in situ observations revealed that these large molecular machines, often referred to as injectiso…
View article: A molecular toolbox to modulate gene expression and protein secretion in the bacterial predator Bdellovibrio bacteriovorus
A molecular toolbox to modulate gene expression and protein secretion in the bacterial predator Bdellovibrio bacteriovorus Open
The predatory bacterium Bdellovibrio bacteriovorus kills and consumes other bacteria, thrives in diverse environments and holds great potential to address major challenges in medicine, agriculture, and biotechnology. As a bacterial predato…
View article: Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis
Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis Open
View article: Yersinia actively downregulates type III secretion and adhesion at higher cell densities
Yersinia actively downregulates type III secretion and adhesion at higher cell densities Open
The T3SS injectisome is used by Gram-negative bacteria, including important pathogens, to manipulate eukaryotic target cells by injecting effector proteins. While in some bacterial species, T3SS-negative bacteria benefit from the activity …
View article: Modulating gene expression and protein secretion in the bacterial predator <i>Bdellovibrio bacteriovorus</i>
Modulating gene expression and protein secretion in the bacterial predator <i>Bdellovibrio bacteriovorus</i> Open
The predatory bacterium Bdellovibrio bacteriovorus kills and consumes other bacteria, thrives in diverse environments and holds great potential to address major challenges in medicine, agriculture, and biotechnology. As a bacterial predato…
View article: Typ VI-Sekretion nach Maß durch horizontalen Gentransfer
Typ VI-Sekretion nach Maß durch horizontalen Gentransfer Open
View article: <i>In situ</i> analysis of type III secretion chaperone proteins indicates a cytosolic handover of virulence effectors
<i>In situ</i> analysis of type III secretion chaperone proteins indicates a cytosolic handover of virulence effectors Open
Gram-negative bacteria can use type III secretion systems to inject effector proteins into eukaryotic target cells. Most effectors are co-expressed with specific chaperone proteins that are required for the secretion of their cognate effec…
View article: Cytosolic delivery of monobodies using the bacterial type III secretion system inhibits oncogenic BCR: ABL1 signaling
Cytosolic delivery of monobodies using the bacterial type III secretion system inhibits oncogenic BCR: ABL1 signaling Open
Background The inability of biologics to pass the plasma membrane prevents their development as therapeutics for intracellular targets. To address the lack of methods for cytosolic protein delivery, we used the type III secretion system (T…
View article: Cytosolic delivery of monobodies using the bacterial type III secretion system inhibits oncogenic BCR::ABL1 signaling
Cytosolic delivery of monobodies using the bacterial type III secretion system inhibits oncogenic BCR::ABL1 signaling Open
Background The inability of biologics to pass the plasma membrane prevents their development as therapeutics for intracellular targets. To address the lack of methods for cytosolic protein delivery, we used the type III secretion system (T…
View article: <i>Yersinia</i> actively downregulates type III secretion and adhesion at higher cell densities
<i>Yersinia</i> actively downregulates type III secretion and adhesion at higher cell densities Open
The T3SS injectisome is used by Gram-negative bacteria, including important pathogens, to manipulate eukaryotic target cells by injecting effector proteins. While in some bacterial species, T3SS-negative bacteria benefit from the activity …
View article: The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression
The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression Open
The transcriptional antisilencer VirB acts as a master regulator of virulence gene expression in the human pathogen Shigella flexneri . It binds DNA sequences ( virS ) upstream of VirB-dependent promoters and counteracts their silencing by…
View article: Pilotins are mobile <scp>T3SS</scp> components involved in assembly and substrate specificity of the bacterial type <scp>III</scp> secretion system
Pilotins are mobile <span>T3SS</span> components involved in assembly and substrate specificity of the bacterial type <span>III</span> secretion system Open
In animal pathogens, assembly of the type III secretion system injectisome requires the presence of so‐called pilotins, small lipoproteins that assist the formation of the secretin ring in the outer membrane. Using a combination of functio…
View article: Cytosolic sorting platform complexes shuttle type III secretion system effectors to the injectisome in Yersinia enterocolitica
Cytosolic sorting platform complexes shuttle type III secretion system effectors to the injectisome in Yersinia enterocolitica Open
Bacteria use type III secretion injectisomes to inject effector proteins into eukaryotic target cells. Recruitment of effectors to the machinery and the resulting export hierarchy involve the sorting platform. These conserved proteins form…
View article: Supplementary Information
Supplementary Information Open
Supplementary Information to Direct binding of type III secretion effectors to cytosolic sorting platform complexes in live bacteria indicates an effector shuttling mechanism
View article: Repertoire and abundance of secreted virulence factors shape the pathogenic capacity of Pseudomonas syringae pv. aptata
Repertoire and abundance of secreted virulence factors shape the pathogenic capacity of Pseudomonas syringae pv. aptata Open
Pseudomonas syringae pv. aptata is a member of the sugar beet pathobiome and the causative agent of leaf spot disease. Like many pathogenic bacteria, P. syringae relies on the secretion of toxins, which manipulate host-pathogen interaction…
View article: A ParDE toxin–antitoxin system is responsible for the maintenance of the Yersinia virulence plasmid but not for type III secretion-associated growth inhibition
A ParDE toxin–antitoxin system is responsible for the maintenance of the Yersinia virulence plasmid but not for type III secretion-associated growth inhibition Open
Many Gram-negative pathogens utilize the type III secretion system (T3SS) to translocate virulence-promoting effector proteins into eukaryotic host cells. The activity of this system results in a severe reduction of bacterial growth and di…
View article: Repertoire and abundance of secreted virulence factors shape the pathogenic capacity of<i>Pseudomonas syringae</i>pv.<i>aptata</i>
Repertoire and abundance of secreted virulence factors shape the pathogenic capacity of<i>Pseudomonas syringae</i>pv.<i>aptata</i> Open
Pseudomonas syringae pv. aptata is a member of the sugar beet pathobiome and the causative agent of leaf spot disease. Like many pathogenic bacteria, P. syringae relies on the secretion of toxins, which manipulate host-pathogen interaction…
View article: MINFLUX imaging of a bacterial molecular machine at nanometer resolution
MINFLUX imaging of a bacterial molecular machine at nanometer resolution Open
The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes or even individual proteins. Recently, minimal photon flux (MI…
View article: Distinct Cytosolic Complexes Containing the Type III Secretion System ATPase Resolved by Three-Dimensional Single-Molecule Tracking in Live Yersinia enterocolitica
Distinct Cytosolic Complexes Containing the Type III Secretion System ATPase Resolved by Three-Dimensional Single-Molecule Tracking in Live Yersinia enterocolitica Open
Injectisomes are membrane-embedded, multiprotein assemblies used by bacterial pathogens to inject virulent effector proteins into eukaryotic host cells. Protein secretion is regulated by cytosolic proteins that dynamically bind and unbind …
View article: Distinct cytosolic complexes containing the type III secretion system ATPase resolved by 3D single-molecule tracking in live<i>Yersinia enterocolitica</i>
Distinct cytosolic complexes containing the type III secretion system ATPase resolved by 3D single-molecule tracking in live<i>Yersinia enterocolitica</i> Open
The membrane-embedded injectisome, the structural component of the virulence-associated type III secretion system (T3SS), is used by gram-negative bacterial pathogens to inject species-specific effector proteins into eukaryotic host cells.…
View article: Presence and absence of type VI secretion systems in bacteria
Presence and absence of type VI secretion systems in bacteria Open
The type VI secretion system (T6SS) is a molecular puncturing device that enables Gram-negative bacteria to kill competitors, manipulate host cells and take up nutrients. Who would want to miss such superpowers? Indeed, many studies show h…
View article: Pilotins are mobile T3SS components involved in assembly and substrate specificity of the bacterial type III secretion system
Pilotins are mobile T3SS components involved in assembly and substrate specificity of the bacterial type III secretion system Open
In animal pathogens, assembly of the type III secretion system injectisome requires the presence of so-called pilotins, small lipoproteins that assist the formation of the secretin ring in the outer membrane. Using a combination of functio…
View article: Journal Club
Journal Club Open
Zellwandmodifkationen generieren Schutzschild gegen AntibiotikumVancomycin ist eines der wichtigsten Reserveantibiotika gegen Methicillinresistente Staphylococcus aureus(MRSA-) Infektionen.
View article: Optogenetics in bacteria – applications and opportunities
Optogenetics in bacteria – applications and opportunities Open
Optogenetics holds the promise of controlling biological processes with superb temporal and spatial resolution at minimal perturbation. Although many of the light-reactive proteins used in optogenetic systems are derived from prokaryotes, …
View article: Gezielte Injektion von Effektoren durch Kontrolle der Proteindynamik
Gezielte Injektion von Effektoren durch Kontrolle der Proteindynamik Open
The type III secretion system (T3SS) enables direct injection of bacterial effector proteins into eukaryotic cells. We found that the dynamic cytosolic interface of the system allows Yersinia enterocolitica to suppress premature secretion …
View article: Visualization of bacterial type 3 secretion system components down to the molecular level by MINFLUX nanoscopy
Visualization of bacterial type 3 secretion system components down to the molecular level by MINFLUX nanoscopy Open
Type 3 secretion systems (T3SS) are essential virulence factors of numerous bacterial pathogens and inject effector proteins into host cells. The needle-like T3SS machinery consists of more than 20 components, has a length of around 100 nm…
View article: Review for "Regulation of bacterial Type III Secretion System export gate opening by substrates and the FliJ stalk of the flagellar ATPase"
Review for "Regulation of bacterial Type III Secretion System export gate opening by substrates and the FliJ stalk of the flagellar ATPase" Open
View article: Author Correction: Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH
Author Correction: Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH Open
View article: Decision letter: Recognition of discrete export signals in early flagellar subunits during bacterial type III secretion
Decision letter: Recognition of discrete export signals in early flagellar subunits during bacterial type III secretion Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Type III Secretion Sys…