Andreas Dolf
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View article: Flow cytometry protocol for cell death analysis in glioblastoma organoids: A technical note
Flow cytometry protocol for cell death analysis in glioblastoma organoids: A technical note Open
Tumor organoid models have emerged as a promising tool in cancer research. By preserving intra- and intertumoral heterogeneity and structural integrity they provide a physiologically relevant platform for drug-response studies. However, va…
Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue Open
Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and remodeling. Macrophage function in WAT is determined by ontogeny and the local tissue environmen…
NEW APPROACH TO SEPARATE AND DATE SMALL SPORES AND POLLEN FROM LAKE SEDIMENTS IN SEMI-ARID CLIMATES Open
The dating of pollen grains is emerging as the method of choice for lacustrine climate archives that contain few datable macrofossils. Due to the need for high-purity pollen concentrates, new methods are constantly being developed to preci…
Meclofenamate causes loss of cellular tethering and decoupling of functional networks in glioblastoma Open
Background Glioblastoma cells assemble to a syncytial communicating network based on tumor microtubes (TMs) as ultra-long membrane protrusions. The relationship between network architecture and transcriptional profile remains poorly invest…
Inhibition of Intercellular Cytosolic Traffic via Gap Junctions Reinforces Lomustine-Induced Toxicity in Glioblastoma Independent of MGMT Promoter Methylation Status Open
Glioblastoma is a malignant brain tumor and one of the most lethal cancers in human. Temozolomide constitutes the standard chemotherapeutic agent, but only shows limited efficacy in glioblastoma patients with unmethylated O-6-methylguanine…
High-Dimensional Analysis of Immune Cell Composition Predicts Periprosthetic Joint Infections and Dissects Its Pathophysiology Open
Accurate diagnosis of periprosthetic joint infections (PJI) is one of the most widely researched areas in modern orthopedic endoprosthesis. However, our understanding of the immunological basis of this severe complication is still limited.…
View article: Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) Open
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors…
Inhibition of Gap Junctions Sensitizes Primary Glioblastoma Cells for Temozolomide Open
Gap junctions have recently been shown to interconnect glioblastoma cells to a multicellular syncytial network, thereby allowing intercellular communication over long distances as well as enabling glioblastoma cells to form routes for brai…
View article: Guidelines for the use of flow cytometry and cell sorting in immunological studies <sup>*</sup>
Guidelines for the use of flow cytometry and cell sorting in immunological studies <sup>*</sup> Open
The marriage between immunology and cytometry is one of the most stable and productive in the recent history of science. A rapid search in PubMed shows that, as of July 2017, using “flow cytometry immunology” as a search term yields more t…