Andreas Winkler
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View article: Coiled-coil register transitions and coupling with the effector’s inhibitory site enables high fold changes in blue light–regulated diguanylate cyclases
Coiled-coil register transitions and coupling with the effector’s inhibitory site enables high fold changes in blue light–regulated diguanylate cyclases Open
Cellular signaling cascades rely on directional transfer of information from one protein to another or within a single protein. To facilitate signal integration, specific structural motifs evolved that not only allow signal processing but …
View article: Major Cat Allergen Fel d 4: Structure and Identification of a Cross‐Reactive <scp>IgE</scp> ‐Epitope‐Containing Area
Major Cat Allergen Fel d 4: Structure and Identification of a Cross‐Reactive <span>IgE</span> ‐Epitope‐Containing Area Open
Background Allergic sensitization to cats and other furry animals is a major cause of asthma and allergic rhinitis in more than 200 million people worldwide. According to the frequency of IgE recognition, allergen‐specific IgE levels, and …
View article: Hydrogen–Deuterium Exchange Coupled to Mass Spectrometry-Based Analysis of Phytochrome Photoreceptors
Hydrogen–Deuterium Exchange Coupled to Mass Spectrometry-Based Analysis of Phytochrome Photoreceptors Open
Red light sensing phytochromes typically have at least two functionally distinct conformations, a red-absorbing Pr state and a far-red-absorbing Pfr state. Understanding the structural differences between these functional states is key to …
View article: Frozen Elephant Trunk in Acute Aortic Syndrome: Retrospective Results from a Low-Volume Center
Frozen Elephant Trunk in Acute Aortic Syndrome: Retrospective Results from a Low-Volume Center Open
Objective: The role of the frozen elephant trunk technique in the treatment of acute aortic dissections is currently based on results from high-volume centers only. We investigated the patient selection process, intraoperative data, the ev…
View article: Integrating protein sequence design and evolutionary sequence conservation to uncover spectral tuning sites in red-light photoreceptors
Integrating protein sequence design and evolutionary sequence conservation to uncover spectral tuning sites in red-light photoreceptors Open
Protein structure and function are defined by non-covalent interactions of the polypeptide backbone and amino acid side chains providing specific chemical environments. Understanding how these interactions impact stability and/or functiona…
View article: A multi-center, open label, single group, observational clinical trial to investigate the effects of training on the administration of Cardioplexol™
A multi-center, open label, single group, observational clinical trial to investigate the effects of training on the administration of Cardioplexol™ Open
Introduction Cardioplexol™ was recently proven effective and non-inferior to Buckberg's solution in a pivotal Phase-3 clinical trial. We hypothesized here that a standardized training program for surgeons without prior experience of Cardio…
View article: Endothelial Activation and Stress Index—A Novel and Simple Prognostic Tool in Coronary Artery Bypass Grafting
Endothelial Activation and Stress Index—A Novel and Simple Prognostic Tool in Coronary Artery Bypass Grafting Open
Objectives: Risk stratification in coronary artery bypass grafting (CABG) remains challenging despite existing models. The Endothelial Activation and Stress Index (EASIX), originally developed for hematological conditions, has shown promis…
View article: <scp>ESM</scp> ‐scan—A tool to guide amino acid substitutions
<span>ESM</span> ‐scan—A tool to guide amino acid substitutions Open
Protein structure prediction and (re)design have gone through a revolution in the last 3 years. The tremendous progress in these fields has been almost exclusively driven by readily available machine learning algorithms applied to protein …
View article: ESM-Scan - a tool to guide amino acid substitutions
ESM-Scan - a tool to guide amino acid substitutions Open
Protein structure prediction and (re)design have gone through a revolution in the last three years. The tremendous progress in these fields has been almost exclusively driven by readily available machine-learning algorithms applied to prot…
View article: Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Open
Regulatory proteins play a crucial role in adaptation to environmental cues. Especially for lifestyle transitions, such as cell proliferation or apoptosis, switch-like characteristics are desirable. While nature frequently uses regulatory …
View article: The structural effect between the output module and chromophore-binding domain is a two-way street via the hairpin extension
The structural effect between the output module and chromophore-binding domain is a two-way street via the hairpin extension Open
Signal transduction typically starts with either ligand binding or cofactor activation, eventually affecting biological activities in the cell. In red light-sensing phytochromes, isomerization of the bilin chromophore results in regulation…
View article: Characterisation of sequence–structure–function space in sensor–effector integrators of phytochrome-regulated diguanylate cyclases
Characterisation of sequence–structure–function space in sensor–effector integrators of phytochrome-regulated diguanylate cyclases Open
Understanding the relationship between protein sequence, structure and function is one of the fundamental challenges in biochemistry. A direct correlation, however, is often not trivial since protein dynamics also play an important functio…
View article: Cord clamping beyond 3 minutes: Neonatal short‐term outcomes and maternal postpartum hemorrhage
Cord clamping beyond 3 minutes: Neonatal short‐term outcomes and maternal postpartum hemorrhage Open
Background Delaying cord clamping (CC) for 3‐5 minutes reduces iron deficiency and improves neurodevelopment. Data on the effects of CC beyond 3 minutes in relation to short‐term neonatal outcomes and maternal risk of postpartum hemorrhage…
View article: Impact of concomitant replacement of the ascending aorta in patients undergoing aortic valve replacement on operative morbidity and mortality
Impact of concomitant replacement of the ascending aorta in patients undergoing aortic valve replacement on operative morbidity and mortality Open
OBJECTIVES The aim of this study was to evaluate the impact of concomitant ascending aortic replacement on operative morbidity and mortality in patients undergoing aortic valve replacement (AVR). METHODS We retrospectively analysed our ins…
View article: The scope of flavin-dependent reactions and processes in the model plant Arabidopsis thaliana
The scope of flavin-dependent reactions and processes in the model plant Arabidopsis thaliana Open
Flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are utilized as coenzymes in many biochemical reduction-oxidation reactions owing to the ability of the tricyclic isoalloxazine ring system to employ the oxidized, radical a…
View article: The Trimeric Major Capsid Protein of Mavirus is stabilized by its Interlocked N-termini Enabling Core Flexibility for Capsid Assembly
The Trimeric Major Capsid Protein of Mavirus is stabilized by its Interlocked N-termini Enabling Core Flexibility for Capsid Assembly Open
Icosahedral viral capsids assemble with high fidelity from a large number of identical buildings blocks. The mechanisms that enable individual capsid proteins to form stable oligomeric units (capsomers) while affording structural adaptabil…
View article: Multicentre experience with the frozen elephant trunk technique to treat penetrating aortic ulcers involving the aortic arch
Multicentre experience with the frozen elephant trunk technique to treat penetrating aortic ulcers involving the aortic arch Open
OBJECTIVES Our goal was to evaluate the use of the frozen elephant trunk (FET) technique for the treatment of penetrating aortic ulcers involving the aortic arch. METHODS Between January 2008 and January 2020, a total of 34 patients had th…
View article: LOVTRAP: an optogenetic system for photoinduced protein dissociation
LOVTRAP: an optogenetic system for photoinduced protein dissociation Open
Here we introduce LOVTRAP, an optogenetic approach for reversible, light-induced protein dissociation. LOVTRAP is based on protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in Kd…
View article: Improving Aortic Repair Outcomes: A New Device for Echocardiographic Valve Assessment During Cardioplegic Arrest
Improving Aortic Repair Outcomes: A New Device for Echocardiographic Valve Assessment During Cardioplegic Arrest Open
Objective: Valve sparing aortic root replacement is a complex surgical procedure. However, unlike for mitral valve repair, there is no possibility to evaluate post repair valve competence during cardioplegic arrest. Our group developed the…
View article: Distinct chromophore–protein environments enable asymmetric activation of a bacteriophytochrome-activated diguanylate cyclase
Distinct chromophore–protein environments enable asymmetric activation of a bacteriophytochrome-activated diguanylate cyclase Open
Sensing of red and far-red light by bacteriophytochromes involves intricate interactions between their bilin chromophore and the protein environment. The light-triggered rearrangements of the cofactor configuration and eventually the prote…
View article: A small molecule chaperone rescues the stability and activity of a cancer‐associated variant of NAD(P)H:quinone oxidoreductase 1 <i>in vitro</i>
A small molecule chaperone rescues the stability and activity of a cancer‐associated variant of NAD(P)H:quinone oxidoreductase 1 <i>in vitro</i> Open
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a human FAD‐dependent enzyme that plays a crucial role in the antioxidant defense system. A naturally occurring single‐nucleotide polymorphism ( NQO1*2 ) in the NQO1 gene leads to an amino acid su…
View article: Comparison of the conventional frozen elephant trunk implantation technique with a modified implantation technique in zone 1
Comparison of the conventional frozen elephant trunk implantation technique with a modified implantation technique in zone 1 Open
OBJECTIVES The aim of the study was to compare the conventional frozen elephant trunk implantation technique with a modified implantation technique with an aortic anastomosis in zone 1 and extra-anatomic revascularization of the left subcl…
View article: The first patient treated with a triple combination therapy after recurrent ischemic stroke
The first patient treated with a triple combination therapy after recurrent ischemic stroke Open
This case report presents the evolution of a patient with recurrent ischemic stroke, in the context of treatment with multimodal agent cerebrolysin, as an add-on to neurological rehabilitation and tDCS therapy. The patient was evaluated be…
View article: Multicentre experience with two frozen elephant trunk prostheses in the treatment of acute aortic dissection†
Multicentre experience with two frozen elephant trunk prostheses in the treatment of acute aortic dissection† Open
OBJECTIVES The aim of this study was to evaluate early- and mid-term outcome and aortic remodelling in patients undergoing implantation of 2 different frozen elephant trunk prostheses, either the Thoraflex™ hybrid (Vascutek, Inchinnan, UK)…