Andrés Binolfi
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View article: Periplasmic protein quality control at atomic level in live cells
Periplasmic protein quality control at atomic level in live cells Open
View article: An inducible and reversible system to regulate unsaturated fatty acid biosynthesis in <i>C. elegans</i>
An inducible and reversible system to regulate unsaturated fatty acid biosynthesis in <i>C. elegans</i> Open
Unsaturated fatty acids (UFAs) play crucial roles in various physiological and pathological processes. In animals, these lipids are synthesized from saturated fatty acids through the action of delta 9 (Δ9) desaturases. In C. elegans, three…
View article: Kinase Mobility Shift Assay (KiMSA) for Assessing Protein Kinase A Activity
Kinase Mobility Shift Assay (KiMSA) for Assessing Protein Kinase A Activity Open
The cAMP-dependent protein kinase (PKA) is one of the most extensively distributed kinases among intracellular signal cascades, with a pivotal role in the regulation of various processes, including the capacitation of sperm cells. Traditio…
View article: G-Quadruplex Structures as Epigenetic Regulatory Elements in Priming of Defense Genes upon Short-Term Trichoderma atroviride Inoculation in Maize
G-Quadruplex Structures as Epigenetic Regulatory Elements in Priming of Defense Genes upon Short-Term Trichoderma atroviride Inoculation in Maize Open
Symbiosis establishment between Trichoderma atroviride and plant roots triggers the priming of defense responses, among other effects. Currently, there is no clear evidence regarding the molecular mechanisms that allow the plant to remain …
View article: A high-resolution 13C NMR approach for profiling fatty acid unsaturation in lipid extracts and in live Caenorhabditis elegans
A high-resolution 13C NMR approach for profiling fatty acid unsaturation in lipid extracts and in live Caenorhabditis elegans Open
Unsaturated fatty acids (UFA) play a crucial role in central cellular processes in animals, including membrane function, development, and disease. Disruptions in UFA homeostasis can contribute to the onset of metabolic, cardiovascular, and…
View article: A versatile kinase mobility shift assay (KiMSA) for PKA analysis and cyclic AMP detection in sperm physiology (and beyond)
A versatile kinase mobility shift assay (KiMSA) for PKA analysis and cyclic AMP detection in sperm physiology (and beyond) Open
The cAMP-dependent protein kinase (PKA) is one of the most extensively distributed kinases among intracellular signal cascades, with a pivotal role in the regulation of various processes, including the capacitation of sperm cells. Traditio…
View article: Genetic variations in G-quadruplex forming sequences affect the transcription of human disease-related genes
Genetic variations in G-quadruplex forming sequences affect the transcription of human disease-related genes Open
Guanine-rich DNA strands can fold into non-canonical four-stranded secondary structures named G-quadruplexes (G4s). G4s folded in proximal promoter regions (PPR) are associated either with positive or negative transcriptional regulation. G…
View article: High resolution protein in-cell NMR in zebrafish embryos
High resolution protein in-cell NMR in zebrafish embryos Open
In-cell NMR spectroscopy has emerged as a powerful tool to evaluate protein conformations and dynamics in the native environment of live cells. Here we extend these studies to a multicellular developing vertebrate. Zebrafish (Danio rerio) …
View article: G-Quadruplexes Regulate miRNA Biogenesis in Live Zebrafish Embryos
G-Quadruplexes Regulate miRNA Biogenesis in Live Zebrafish Embryos Open
RNA guanine quadruplexes (G4s) regulate RNA functions, metabolism, and processing. G4s formed within precursors of microRNAs (pre-miRNAs) may impair pre-miRNAs maturation by Dicer, thus repressing mature miRNA biogenesis. As miRNAs are ess…
View article: G-quadruplexes regulate miRNA biogenesis in live zebrafish embryos
G-quadruplexes regulate miRNA biogenesis in live zebrafish embryos Open
RNA guanine quadruplexes (G4s) regulate RNA functions, metabolism, and processing. G4s formed within precursors of microRNAs (pre-miRNAs) may impair pre-miRNAs maturation by Dicer, thus repressing mature miRNA biogenesis. As miRNAs are ess…
View article: Genetic variations in G-Quadruplex forming sequences affect the transcription of human disease-related genes
Genetic variations in G-Quadruplex forming sequences affect the transcription of human disease-related genes Open
Guanine-rich DNA strands can fold into non-canonical four-stranded secondary structures named G-quadruplexes (G4s). G4s folded in proximal promoter regions (PPR) are associated either with positive or negative transcriptional regulation. G…
View article: Unsaturated fatty acids profiling in live<i>C. elegans</i>using real-time NMR spectroscopy
Unsaturated fatty acids profiling in live<i>C. elegans</i>using real-time NMR spectroscopy Open
Unsaturated fatty acids (UFAs) impact central cellular process in animals such as membrane function, development and disease. Perturbations of UFAs homeostasis contribute to the onset of metabolic, cardiovascular and neurodegenerative diso…
View article: Structural features of the plant N‐recognin ClpS1 and sequence determinants in its targets that govern substrate selection
Structural features of the plant N‐recognin ClpS1 and sequence determinants in its targets that govern substrate selection Open
In the N‐degron pathway of protein degradation of Escherichia coli , the N‐recognin ClpS identifies substrates bearing N‐terminal phenylalanine, tyrosine, tryptophan, or leucine and delivers them to the caseinolytic protease (Clp). Chlorop…
View article: CNBP Binds and Unfolds In Vitro G-Quadruplexes Formed in the SARS-CoV-2 Positive and Negative Genome Strands
CNBP Binds and Unfolds In Vitro G-Quadruplexes Formed in the SARS-CoV-2 Positive and Negative Genome Strands Open
The Coronavirus Disease 2019 (COVID-19) pandemic has become a global health emergency with no effective medical treatment and with incipient vaccines. It is caused by a new positive-sense RNA virus called severe acute respiratory syndrome-…
View article: Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs)
Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs) Open
View article: Megadalton-sized Dityrosine Aggregates of α-Synuclein Retain High Degrees of Structural Disorder and Internal Dynamics
Megadalton-sized Dityrosine Aggregates of α-Synuclein Retain High Degrees of Structural Disorder and Internal Dynamics Open
Heterogeneous aggregates of the human protein α-synuclein (αSyn) are abundantly found in Lewy body inclusions of Parkinson's disease patients. While structural information on classical αSyn amyloid fibrils is available, little is known abo…
View article: Megadalton-sized dityrosine aggregates of α-synuclein retain high degrees of structural disorder and internal dynamics
Megadalton-sized dityrosine aggregates of α-synuclein retain high degrees of structural disorder and internal dynamics Open
Heterogeneous aggregates of the human protein α-synuclein (αSyn) are abundantly found in Lewy body inclusions of Parkinson’s disease patients. While structural information on classical αSyn amyloid fibrils is available, little is known abo…
View article: An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities<i>in vitro</i>and<i>in vivo</i>
An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities<i>in vitro</i>and<i>in vivo</i> Open
Oxidation of protein methionines to methionine-sulfoxides (MetOx) is associated with several age-related diseases. In healthy cells, MetOx is reduced to methionine by two families of conserved methionine sulfoxide reductase enzymes, MSRA a…
View article: Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 Open
Cells continuously adapt cellular processes by integrating external and internal signals. In yeast, multiple stress signals regulate pheromone signaling to prevent mating under unfavorable conditions. However, the underlying crosstalk mech…
View article: CNBP controls transcription by unfolding DNA G-quadruplex structures
CNBP controls transcription by unfolding DNA G-quadruplex structures Open
Guanine-rich DNA strands can fold into non-canonical four-stranded secondary structures named G-quadruplexes (G4). Experimental evidences suggest that G4-DNA surrounding transcription start sites act as cis-regulatory elements by either st…
View article: Repurposing doxycycline for synucleinopathies: remodelling of α-synuclein oligomers towards non-toxic parallel beta-sheet structured species
Repurposing doxycycline for synucleinopathies: remodelling of α-synuclein oligomers towards non-toxic parallel beta-sheet structured species Open
View article: Intracellular repair of oxidation-damaged α-synuclein fails to target C-terminal modification sites
Intracellular repair of oxidation-damaged α-synuclein fails to target C-terminal modification sites Open
Cellular oxidative stress serves as a common denominator in many neurodegenerative disorders, including Parkinson’s disease. Here we use in-cell NMR spectroscopy to study the fate of the oxidation-damaged Parkinson’s disease protein alpha-…
View article: Structural disorder of monomeric α-synuclein persists in mammalian cells
Structural disorder of monomeric α-synuclein persists in mammalian cells Open
View article: Erratum: Corrigendum: Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts
Erratum: Corrigendum: Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts Open
View article: Thermodynamics of protein destabilization in live cells
Thermodynamics of protein destabilization in live cells Open
Significance A key question in structural biology is how protein properties mapped out under simplified conditions in vitro transfer to the complex environment in live cells. The answer, it appears, varies. Defying predictions from steric …
View article: Copper Binding to the N-Terminally Acetylated, Naturally Occurring Form of Alpha-Synuclein Induces Local Helical Folding
Copper Binding to the N-Terminally Acetylated, Naturally Occurring Form of Alpha-Synuclein Induces Local Helical Folding Open
Growing evidence supports a link between brain copper homeostasis, the formation of alpha-synuclein (AS)-copper complexes, and the development of Parkinson disease (PD). Recently it was demonstrated that the physiological form of AS is N-t…
View article: Real-time NMR monitoring of biological activities in complex physiological environments
Real-time NMR monitoring of biological activities in complex physiological environments Open