Andrew Bowie
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View article: Elongator is required for pattern recognition receptor and type I interferon signaling in macrophages
Elongator is required for pattern recognition receptor and type I interferon signaling in macrophages Open
Innate immune detection of pathogens by macrophages requires a rapid and large scale mobilization of cellular resources towards an appropriate immune response, mainly involving altered protein expression. Pathogen recognition is mediated b…
View article: Chemical- and photo-activation of protein-protein thiol-ene coupling for protein profiling
Chemical- and photo-activation of protein-protein thiol-ene coupling for protein profiling Open
The thiol-ene reaction between an alkene and a thiol can be exploited for selective labelling of cysteine residues in protein profiling applications. Here, we explore thiol-ene activation in systems from chemical models to complex cellular…
View article: Molluscum contagiosum virus protein MC089 inhibits interferon regulatory factor 3 activation
Molluscum contagiosum virus protein MC089 inhibits interferon regulatory factor 3 activation Open
Molluscum contagiosum virus (MCV) is a human-specific poxvirus that causes a highly common but mild infection characterized by distinctive and persistent papular skin lesions. These lesions can persist for long periods without an effective…
View article: Multi-omics characterization of the monkeypox virus infection
Multi-omics characterization of the monkeypox virus infection Open
Multiple omics analyzes of Vaccinia virus (VACV) infection have defined molecular characteristics of poxvirus biology. However, little is known about the monkeypox (mpox) virus (MPXV) in humans, which has a different disease manifestation …
View article: Human Coronavirus 229E Infection Inactivates Pyroptosis Executioner Gasdermin D but Ultimately Leads to Lytic Cell Death Partly Mediated by Gasdermin E
Human Coronavirus 229E Infection Inactivates Pyroptosis Executioner Gasdermin D but Ultimately Leads to Lytic Cell Death Partly Mediated by Gasdermin E Open
Human coronavirus 229E (HCoV-229E) is associated with upper respiratory tract infections and generally causes mild respiratory symptoms. HCoV-229E infection can cause cell death, but the molecular pathways that lead to virus-induced cell d…
View article: SARM1 regulates pro-inflammatory cytokine expression in human monocytes by NADase-dependent and -independent mechanisms
SARM1 regulates pro-inflammatory cytokine expression in human monocytes by NADase-dependent and -independent mechanisms Open
SARM1 is a Toll-IL-1 receptor (TIR) domain-containing protein with roles in innate immunity and neuronal death in diverse organisms. Unlike other innate immune TIR proteins that function as adaptors for Toll-like receptors (TLRs), SARM1 ha…
View article: Regulation of type I and type III interferon induction in response to pathogen sensing
Regulation of type I and type III interferon induction in response to pathogen sensing Open
Type I and III interferons (IFN-I and IFN-III) have a central role in the early antimicrobial response against invading pathogens. Induction of IFN-Is and IFN-IIIs arises due to the sensing by pattern recognition receptors of pathogen-asso…
View article: Human coronavirus 229E infection inactivates pyroptosis executioner gasdermin D but ultimately leads to lytic cell death partly mediated by gasdermin E
Human coronavirus 229E infection inactivates pyroptosis executioner gasdermin D but ultimately leads to lytic cell death partly mediated by gasdermin E Open
Human coronavirus 229E (HCoV-229E) is associated with upper respiratory tract infections and causes local respiratory symptoms. It has been reported that HCoV-229E can cause cell death in a variety of cells in vitro . However, the molecula…
View article: SARM1 regulates NAD+-linked metabolism and select immune genes in macrophages
SARM1 regulates NAD+-linked metabolism and select immune genes in macrophages Open
Sterile alpha and HEAT/armadillo motif-containing protein (SARM1) was recently described as a NAD+-consuming enzyme and has previously been shown to regulate immune responses in macrophages. Neuronal SARM1 is known to contribute to axon de…
View article: PYHIN protein IFI207 regulates cytokine transcription and IRF7 and contributes to the establishment of K. pneumoniae infection
PYHIN protein IFI207 regulates cytokine transcription and IRF7 and contributes to the establishment of K. pneumoniae infection Open
PYHIN proteins AIM2 and IFI204 sense pathogen DNA, while other PYHINs have been shown to regulate host gene expression through as-yet unclear mechanisms. We characterize mouse PYHIN IFI207, which we find is not involved in DNA sensing but …
View article: Molluscum Contagiosum Virus Protein MC008 Targets NF-κB Activation by Inhibiting Ubiquitination of NEMO
Molluscum Contagiosum Virus Protein MC008 Targets NF-κB Activation by Inhibiting Ubiquitination of NEMO Open
Inflammation lies at the heart of most human diseases. Understanding the pathways that drive this response is the key to new anti-inflammatory therapies.
View article: Phelan et al. 2023 Open Access Data Numerical.xlsx
Phelan et al. 2023 Open Access Data Numerical.xlsx Open
This section contains numerical data used in the manuscript titled "Molluscum Contagiosum Virus Protein MC008 targets NF-κB Activation by Inhibiting Ubiquitination of NEMO"
View article: Phelan et al. 2023 Data Availability.xlsx
Phelan et al. 2023 Data Availability.xlsx Open
This section contains numerical data and whole immunoblots used in the manuscripte titled "Molluscum Contagiosum Virus Protein MC008 targets NF-κB Activation by Inhibiting Ubiquitination of NEMO"
View article: Phelan et al. 2023 Data Availability Numerical.xlsx
Phelan et al. 2023 Data Availability Numerical.xlsx Open
This section contains numerical data used in the manuscript titled "Molluscum Contagiosum Virus Protein MC008 targets NF-κB Activation by Inhibiting Ubiquitination of NEMO"
View article: Reactive Centre Loop Mutagenesis of SerpinB3 to Target TMPRSS2 and Furin: Inhibition of SARS-CoV-2 Cell Entry and Replication
Reactive Centre Loop Mutagenesis of SerpinB3 to Target TMPRSS2 and Furin: Inhibition of SARS-CoV-2 Cell Entry and Replication Open
The SARS-CoV-2 virus can utilize host cell proteases to facilitate cell entry, whereby the Spike (S) protein is cleaved at two specific sites to enable membrane fusion. Furin, transmembrane protease serine 2 (TMPRSS2), and cathepsin L (Cat…
View article: Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity
Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity Open
Many bacterial pathogens antagonize host defense responses by translocating effector proteins into cells. It remains an open question how those pathogens not encoding effectors counteract anti-bacterial immunity. Here, we show that Klebsie…
View article: SARM1 Promotes Photoreceptor Degeneration in an Oxidative Stress Model of Retinal Degeneration
SARM1 Promotes Photoreceptor Degeneration in an Oxidative Stress Model of Retinal Degeneration Open
SARM1 (sterile alpha and armadillo motif-containing protein) is a highly conserved Toll/IL-1 Receptor (TIR) adaptor with important roles in mediating immune responses. Studies in the brain have shown that SARM1 plays a role in induction of…
View article: SARM1 Ablation Is Protective and Preserves Spatial Vision in an In Vivo Mouse Model of Retinal Ganglion Cell Degeneration
SARM1 Ablation Is Protective and Preserves Spatial Vision in an In Vivo Mouse Model of Retinal Ganglion Cell Degeneration Open
The challenge of developing gene therapies for genetic forms of blindness is heightened by the heterogeneity of these conditions. However, mechanistic commonalities indicate key pathways that may be targeted in a gene-independent approach.…
View article: Candidate Role for Toll-like Receptor 3 L412F Polymorphism and Infection in Acute Exacerbation of Idiopathic Pulmonary Fibrosis
Candidate Role for Toll-like Receptor 3 L412F Polymorphism and Infection in Acute Exacerbation of Idiopathic Pulmonary Fibrosis Open
Rationale: The Toll-like receptor 3 Leu412Phe (TLR3 L412F) polymorphism attenuates cellular antiviral responses and is associated with accelerated disease progression in idiopathic pulmonary fibrosis (IPF). The role of TLR3 L412F in bacter…
View article: Dual NADPH oxidases DUOX1 and DUOX2 synthesize NAADP and are necessary for Ca <sup>2+</sup> signaling during T cell activation
Dual NADPH oxidases DUOX1 and DUOX2 synthesize NAADP and are necessary for Ca <sup>2+</sup> signaling during T cell activation Open
The enzymes DUOX1 and DUOX2 may mediate rapid and then sustained NAADP production to promote Ca 2+ signaling in T cells.
View article: CRISPR/Cas9-mediated SARM1 knockout and epitope-tagged mice reveal that SARM1 does not regulate nuclear transcription, but is expressed in macrophages
CRISPR/Cas9-mediated SARM1 knockout and epitope-tagged mice reveal that SARM1 does not regulate nuclear transcription, but is expressed in macrophages Open
SARM1 is a toll/interleukin-1 receptor -domain containing protein, with roles proposed in both innate immunity and neuronal degeneration. Murine SARM1 has been reported to regulate the transcription of chemokines in both neurons and macrop…
View article: <i>Klebsiella pneumoniae</i>hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity
<i>Klebsiella pneumoniae</i>hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity Open
SUMMARY Many bacterial pathogens antagonize host defence responses by translocating effector proteins into cells. It remains an open question how those pathogens not encoding effectors counteract anti-bacterial immunity. Here, we show that…
View article: Next generation SARM1 knockout and epitope tagged CRISPR-Cas9-generated isogenic mice reveal that SARM1 does not participate in regulating nuclear transcription, despite confirmation of protein expression in macrophages
Next generation SARM1 knockout and epitope tagged CRISPR-Cas9-generated isogenic mice reveal that SARM1 does not participate in regulating nuclear transcription, despite confirmation of protein expression in macrophages Open
SARM1 is an ancient and highly conserved TIR-domain containing protein, with a diverse range of proposed roles in both innate immunity and neuronal death and degeneration. Murine SARM1 has been reported to regulate the transcription of spe…