Andrew Dippel
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View article: Accelerating high-concentration monoclonal antibody development with large-scale viscosity data and ensemble deep learning
Accelerating high-concentration monoclonal antibody development with large-scale viscosity data and ensemble deep learning Open
Highly concentrated antibody solutions are necessary for developing subcutaneous injections but often exhibit high viscosities, posing challenges in antibody-drug development, manufacturing, and administration. Previous computational model…
View article: Preemptive optimization of a clinical antibody for broad neutralization of SARS-CoV-2 variants and robustness against viral escape
Preemptive optimization of a clinical antibody for broad neutralization of SARS-CoV-2 variants and robustness against viral escape Open
Most previously authorized clinical antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have lost neutralizing activity to recent variants due to rapid viral evolution. To mitigate such escape, we preemptively e…
View article: IL-2-armored peptide-major histocompatibility class I bispecific antibodies redirect antiviral effector memory CD8+ T cells to induce potent anti-cancer cytotoxic activity with limited cytokine release
IL-2-armored peptide-major histocompatibility class I bispecific antibodies redirect antiviral effector memory CD8+ T cells to induce potent anti-cancer cytotoxic activity with limited cytokine release Open
T cell engagers (TCEs) are becoming an integral class of biological therapeutic owing to their highly potent ability to eradicate cancer cells. Nevertheless, the widespread utility of classical CD3-targeted TCEs has been limited by narrow …
View article: Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen
Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen Open
Over the past two decades, therapeutic antibodies have emerged as a rapidly expanding domain within the field of biologics. In silico tools that can streamline the process of antibody discovery and optimization are critical to suppo…
View article: Enhancement of antibody thermostability and affinity by computational design in the absence of antigen
Enhancement of antibody thermostability and affinity by computational design in the absence of antigen Open
Over the last two decades, therapeutic antibodies have emerged as a rapidly expanding domain within the field biologics. In silico tools that can streamline the process of antibody discovery and optimization are critical to support a pipel…
View article: 1355. The SARS-CoV-2 Monoclonal Antibody AZD3152 Potently Neutralizes Historical and Emerging Variants and is Being Developed for the Prevention and Treatment of COVID-19 in High-risk Individuals
1355. The SARS-CoV-2 Monoclonal Antibody AZD3152 Potently Neutralizes Historical and Emerging Variants and is Being Developed for the Prevention and Treatment of COVID-19 in High-risk Individuals Open
Background EVUSHELD was developed for the prevention and treatment of mild-to-moderate COVID-19 for high-risk individuals. However, the SARS-CoV-2 virus continues to evolve in the presence of natural- and vaccine-acquired immunity, escapin…
View article: Robust production of monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the C <sub>H</sub> 1-C <sub>λ</sub> interface
Robust production of monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the C <sub>H</sub> 1-C <sub>λ</sub> interface Open
Bispecific antibodies represent an increasingly large fraction of biologics in therapeutic development due to their expanded scope in functional capabilities. Asymmetric monovalent bispecific IgGs (bsIgGs) have the additional advantage of …
View article: A scalable and high yielding SARS-CoV-2 spike protein receptor binding domain production process
A scalable and high yielding SARS-CoV-2 spike protein receptor binding domain production process Open
The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) spike protein is of interest for the development of vaccines and therapeutics against COVID-19. Vaccines are designed to raise an immune response against the spike protein. O…
View article: Developability profiling of a panel of Fc engineered SARS-CoV-2 neutralizing antibodies
Developability profiling of a panel of Fc engineered SARS-CoV-2 neutralizing antibodies Open
To combat the COVID-19 pandemic, potential therapies have been developed and moved into clinical trials at an unprecedented pace. Some of the most promising therapies are neutralizing antibodies against SARS-CoV-2. In order to maximize the…
View article: Tuning the Innate Immune Response to Cyclic Dinucleotides by Using Atomic Mutagenesis
Tuning the Innate Immune Response to Cyclic Dinucleotides by Using Atomic Mutagenesis Open
Cyclic dinucleotides (CDNs) trigger the innate immune response in eukaryotic cells through the stimulator of interferon genes (STING) signaling pathway. To decipher this complex cellular process, a better correlation between structure and …
View article: Development of Ratiometric Bioluminescent Sensors for <i>in Vivo</i> Detection of Bacterial Signaling
Development of Ratiometric Bioluminescent Sensors for <i>in Vivo</i> Detection of Bacterial Signaling Open
Second messenger signaling networks allow cells to sense and adapt to changing environmental conditions. In bacteria, the nearly ubiquitous second messenger molecule cyclic di-GMP coordinates diverse processes such as motility, biofilm for…
View article: Chemiluminescent sensors for quantitation of the bacterial second messenger cyclic di-GMP
Chemiluminescent sensors for quantitation of the bacterial second messenger cyclic di-GMP Open
View article: Ratiometric bioluminescent sensors towards <i>in vivo</i> imaging of bacterial signaling
Ratiometric bioluminescent sensors towards <i>in vivo</i> imaging of bacterial signaling Open
Second messenger signaling networks allow cells to sense and adapt to changing environmental conditions. In bacteria, the nearly ubiquitous second messenger molecule cyclic di-GMP coordinates diverse processes such as motility, biofilm for…
View article: Development of bioluminescent sensors for interrogating cyclic di-nucleotide signaling
Development of bioluminescent sensors for interrogating cyclic di-nucleotide signaling Open
Bioluminescence is the spectacular natural phenomenon in which living organisms produce and emit light. Natural bioluminescent protein systems have been discovered and characterized in over 30 species, ranging from fireflies to fungi and b…
View article: Chemiluminescent Biosensors for Detection of Second Messenger Cyclic di-GMP
Chemiluminescent Biosensors for Detection of Second Messenger Cyclic di-GMP Open
Bacteria colonize highly diverse and complex environments, from gastrointestinal tracts to soil and plant surfaces. This colonization process is controlled in part by the intracellular signal cyclic di-GMP, which regulates bacterial motili…
View article: In Vivo Biochemistry: Single-Cell Dynamics of Cyclic Di-GMP in <i>Escherichia coli</i> in Response to Zinc Overload
In Vivo Biochemistry: Single-Cell Dynamics of Cyclic Di-GMP in <i>Escherichia coli</i> in Response to Zinc Overload Open
Intracellular signaling enzymes drive critical changes in cellular physiology and gene expression, but their endogenous activities in vivo remain highly challenging to study in real time and for individual cells. Here we show that flow cyt…
View article: Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study
Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study Open
The X-ray crystal structures of superfolder green fluorescent protein (sfGFP) containing the spectroscopic reporter unnatural amino acids (UAAs) 4-cyano-L-phenylalanine (pCNF) or 4-ethynyl-L-phenylalanine (pCCF) at two unique sites in the …
View article: Probing Structural Implications of Unnatural Amino Acid Incorporation into Green Fluorescent Protein
Probing Structural Implications of Unnatural Amino Acid Incorporation into Green Fluorescent Protein Open