Andrew Hemphill
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View article: Pleiotropic Effects on Tachyzoite and Host Cell Proteomes in Knock-Out Clones of the Open Reading Frames 297720 and 319730 Constitutively Expressed in T. gondii ShSp1 Tachyzoites
Pleiotropic Effects on Tachyzoite and Host Cell Proteomes in Knock-Out Clones of the Open Reading Frames 297720 and 319730 Constitutively Expressed in T. gondii ShSp1 Tachyzoites Open
Toxoplasma gondii, the causative agent of toxoplasmosis widespread in animals and humans, is an intracellular apicomplexan protozoan parasite infecting a variety of host cells. Gene editing using CRISPR-Cas9 has become a standard tool to i…
View article: Dose-dependent tissue tropism and efficacy of early BKI-1748 treatment in chronic Toxoplasma gondii infection in sheep
Dose-dependent tissue tropism and efficacy of early BKI-1748 treatment in chronic Toxoplasma gondii infection in sheep Open
View article: Bumped kinase inhibitor BKI-1708 interferes in cytokinesis and drives baryzoite stage conversion in the cyst-forming apicomplexan parasites <i>Toxoplasma gondii, Neospora caninum</i> and <i>Besnoitia besnoiti</i>
Bumped kinase inhibitor BKI-1708 interferes in cytokinesis and drives baryzoite stage conversion in the cyst-forming apicomplexan parasites <i>Toxoplasma gondii, Neospora caninum</i> and <i>Besnoitia besnoiti</i> Open
Bumped kinase inhibitors (BKIs) have demonstrated safety and promising efficacy against various apicomplexan pathogens both in vitro and in vivo . However, in the closely related cyst-forming coccidians T. gondii , Neospora caninum and Bes…
View article: Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum
Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum Open
View article: <p class="MDPI13authornames" style="margin-bottom: 12.0pt;">Pleiotropic Effects on Tachyzoite and Host Cell Proteomes in Knock-Out Clones of the Open Reading Frames 297720 and 319730 Constitutively Expressed in <em>T. gondii</em> ShSp1 Tachyzoites
Pleiotropic Effects on Tachyzoite and Host Cell Proteomes in Knock-Out Clones of the Open Reading Frames 297720 and 319730 Constitutively Expressed in <em>T. gondii</em> ShSp1 Tachyzoites Open
Toxoplasma gondii, the causative agent of toxoplasmosis widespread in animals and humans, is an intracellular apicomplexan protozoan parasite infecting a variety of host cells. Gene editing using CRISPR-Cas9 has become a standard tool to i…
View article: Proteome changes during in vitro culture adaptation of Toxoplasma gondii archetypal II and III field isolates
Proteome changes during in vitro culture adaptation of Toxoplasma gondii archetypal II and III field isolates Open
Introduction Rapid in vitro culture adaptation of recently obtained Toxoplasma gondii isolates leading to deep changes in relevant phenotypic traits has been demonstrated earlier. Few reports exist on the molecular bases that govern this a…
View article: In vitro screening of the open-access Pandemic Response Box reveals ESI-09 as a compound with activity against Echinococcus multilocularis
In vitro screening of the open-access Pandemic Response Box reveals ESI-09 as a compound with activity against Echinococcus multilocularis Open
Alveolar echinococcosis (AE) is a life-threatening disease caused by the metacestode stage of the fox tapeworm Echinococcus multilocularis, primarily in the liver. Current drug treatments rely on benzimidazoles, which are not parasiticidal…
View article: Discovery and rescue of porcine bastroviruses associated with polioencephalomyelitis in domestic pigs
Discovery and rescue of porcine bastroviruses associated with polioencephalomyelitis in domestic pigs Open
Bastroviruses (BastV) are non-enveloped single-stranded positive-sense RNA viruses that have been discovered recently in feces samples of different animals and humans. The non-structural proteins of these viruses show similarities to those…
View article: In Vivo Safety and Efficacy of Thiosemicarbazones in Experimental Mice Infected with Toxoplasma gondii Oocysts
In Vivo Safety and Efficacy of Thiosemicarbazones in Experimental Mice Infected with Toxoplasma gondii Oocysts Open
Background: Toxoplasma gondii is a globally widespread parasite responsible for toxoplasmosis, a zoonotic disease with significant impact on both human and animal health. The current lack of safe and effective treatments underscores the ne…
View article: Anti-Cryptosporidium efficacy of BKI-1708, an inhibitor of Cryptosporidium calcium-dependent protein kinase 1
Anti-Cryptosporidium efficacy of BKI-1708, an inhibitor of Cryptosporidium calcium-dependent protein kinase 1 Open
Background Diarrheal pathogens, such as Cryptosporidium, impose a heavy burden of disease in resource-limited regions. Cryptosporidiosis often causes chronic infection in immunocompromised people and gastrointestinal injury in malnourished…
View article: Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland Open
Sarcocystis spp. and Toxoplasma gondii are heteroxenous protozoan parasites, which form cysts in muscle tissue of intermediate hosts (IH) and oocysts in the intestinal mucosa of definitive hosts (DH). Wild boars (Sus scrof…
View article: Divergent host–pathogen interactions in neurolisteriosis: cytosolic replication vs. phagosomal dormancy of Listeria monocytogenes in CNS macrophages
Divergent host–pathogen interactions in neurolisteriosis: cytosolic replication vs. phagosomal dormancy of Listeria monocytogenes in CNS macrophages Open
View article: Human Alveolar Echinococcosis—A Neglected Zoonotic Disease Requiring Urgent Attention
Human Alveolar Echinococcosis—A Neglected Zoonotic Disease Requiring Urgent Attention Open
Alveolar echinococcosis (AE) in humans is caused by the larval (metacestode) stage of Echinococcus multilocularis, commonly known as the ‘fox tapeworm’. This disease predominantly targets the liver and has an invasive growth pattern, allow…
View article: Intravacuolar persistence in neutrophils facilitates <i>Listeria monocytogenes</i> spread to co-cultured cells
Intravacuolar persistence in neutrophils facilitates <i>Listeria monocytogenes</i> spread to co-cultured cells Open
The bacterium Listeria monocytogenes ( Lm ) causes listeriosis in humans and ruminants. Acute lesions are predominantly infiltrated by polymorphonuclear neutrophils (PMNs), considered to be the efficient bactericidal arm of innate immunity…
View article: Preimaginal development of <i>Aedes aegypti</i> L. (Diptera: Culicidae) in brackish water gives rise to adult mosquitoes with thicker cuticles and greater insecticide resistance
Preimaginal development of <i>Aedes aegypti</i> L. (Diptera: Culicidae) in brackish water gives rise to adult mosquitoes with thicker cuticles and greater insecticide resistance Open
Aedes aegypti L. and Aedes albopictus Skuse mosquitoes (Diptera: Culicidae), the principal vectors of many human arboviral diseases, lay eggs and undergo preimaginal development in fresh water. They have recently been shown to also develop…
View article: Human alveolar echinococcosis–global, regional, and national annual incidence and prevalence rates
Human alveolar echinococcosis–global, regional, and national annual incidence and prevalence rates Open
The AE burden in humans is substantial and emphasises the need for enhanced public awareness, surveillance, prevention, and control, particularly in under-resourced communities to mitigate the health, economic, and psychological impact of …
View article: Correction: Morphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef
Correction: Morphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef Open
View article: Respiratory and intestinal zoonotic cryptosporidiosis in symptomatic domestic pigeons (<i>Columba livia domestica</i>) in Tabriz, Iran.
Respiratory and intestinal zoonotic cryptosporidiosis in symptomatic domestic pigeons (<i>Columba livia domestica</i>) in Tabriz, Iran. Open
Considering the high frequency of cryptosporidiosis reported here in symptomatic birds and that both identified Cryptosporidium species are zoonotic parasites, findings claim a public health risk assessment of this species of animals.
View article: Treatment with BKI-1748 after <i>Toxoplasma gondii</i> systemic dissemination in experimentally infected pregnant sheep improves fetal and lamb mortality and morbidity and prevents congenital infection
Treatment with BKI-1748 after <i>Toxoplasma gondii</i> systemic dissemination in experimentally infected pregnant sheep improves fetal and lamb mortality and morbidity and prevents congenital infection Open
Drug development for congenital toxoplasmosis is challenging since first-line therapy has a high rate of adverse effects and exhibits suboptimal efficacy. Bumped kinase inhibitors (BKIs), targeting protein kinases with small gatekeeper res…
View article: Morphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef
Morphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef Open
Background Parasites in the apicomplexan genus Sarcocystis infect cattle worldwide. Assessing the economic importance of each such parasite species requires proper diagnosis. Sarcocystis cruzi, a thin-walled species, infects virtually all …
View article: Novel zoonotic Enterocytozoon and Encephalitozoon genotypes in domestic pigeons (Columba livia domestica) in Iran: Public health implications
Novel zoonotic Enterocytozoon and Encephalitozoon genotypes in domestic pigeons (Columba livia domestica) in Iran: Public health implications Open
To determine the occurrence of microsporidiosis in domestic pigeons in Iran, privately-owned pigeons presenting weight loss and diarrhea were tested through molecular and histopathological methods. Multiplex-PCR findings showed 57%, 30%, a…
View article: Transient Adaptation of Toxoplasma gondii to Exposure by Thiosemicarbazone Drugs That Target Ribosomal Proteins Is Associated with the Upregulated Expression of Tachyzoite Transmembrane Proteins and Transporters
Transient Adaptation of Toxoplasma gondii to Exposure by Thiosemicarbazone Drugs That Target Ribosomal Proteins Is Associated with the Upregulated Expression of Tachyzoite Transmembrane Proteins and Transporters Open
Thiosemicarbazones and their metal complexes have been studied for their biological activities against bacteria, cancer cells and protozoa. Short-term in vitro treatment with one gold (III) complex (C3) and its salicyl-thiosemicarbazone li…
View article: Predatory Potential of Nymphal Odonates on Aedes aegypti Developing in Freshwater and Brackish Water Habitats
Predatory Potential of Nymphal Odonates on Aedes aegypti Developing in Freshwater and Brackish Water Habitats Open
Aedes aegypti, the primary vector of dengue, undergoes preimaginal development in brackish water (BW). However, dengue vector control exclusively targets freshwater (FW) habitats. The present study evaluated the predatory efficacy of nymph…
View article: Preimaginal development of<i>Aedes aegypti</i>in brackish water produces adult mosquitoes with thicker cuticles and greater insecticide resistance
Preimaginal development of<i>Aedes aegypti</i>in brackish water produces adult mosquitoes with thicker cuticles and greater insecticide resistance Open
Aedes aegypti and Aedes albopictus mosquitoes, the principal vectors of many human arboviral diseases, lay eggs and undergo preimaginal development in fresh water habitats. They have also recently been found to develop in brackish water in…
View article: Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools—Chances and Limitations: A Critical Review
Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools—Chances and Limitations: A Critical Review Open
Identification of drug targets and biochemical investigations on mechanisms of action are major issues in modern drug development. The present article is a critical review of the classical “one drug”—“one target” paradigm. In fact, novel m…
View article: TaqMan-quantitative PCR assays applied in Neospora caninum knock-outs generated through CRISPR-Cas9 allow to determine the copy numbers of integrated dihydrofolate reductase-thymidylate synthase drug selectable markers
TaqMan-quantitative PCR assays applied in Neospora caninum knock-outs generated through CRISPR-Cas9 allow to determine the copy numbers of integrated dihydrofolate reductase-thymidylate synthase drug selectable markers Open
As for many other organisms, CRISPR-Cas9 mediated genetic modification has gained increasing importance for the identification of vaccine candidates and drug targets in Neospora caninum , an apicomplexan parasite causing abortion in cattle…
View article: Efficacy of the bumped kinase inhibitor BKI-1708 against the cyst-forming apicomplexan parasites Toxoplasma gondii and Neospora caninum in vitro and in experimentally infected mice
Efficacy of the bumped kinase inhibitor BKI-1708 against the cyst-forming apicomplexan parasites Toxoplasma gondii and Neospora caninum in vitro and in experimentally infected mice Open
Toxoplasma gondii and Neospora caninum are major worldwide morbidity-causing pathogens. Bumped kinase inhibitors (BKIs) are a compound class that has been optimized to target the apicomplexan calcium-dependent protein kinase 1 (CDPK1) - an…
View article: Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools – Chances and Limitations. A Critical Review
Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools – Chances and Limitations. A Critical Review Open
Identification of drug targets and biochemical investigations on mechanisms of action is a are major issues in modern drug development. The present article reviews the classical “one drug” – “one target” parad…
View article: In vitro and in vivo activities of a trithiolato-diRuthenium complex conjugated with sulfadoxine against the apicomplexan parasite Toxoplasma gondii
In vitro and in vivo activities of a trithiolato-diRuthenium complex conjugated with sulfadoxine against the apicomplexan parasite Toxoplasma gondii Open
Organometallic compounds, including Ruthenium complexes, have been widely developed as anti-cancer chemotherapeutics, but have also attracted much interest as potential anti-parasitic drugs. Recently hybrid drugs composed of organometallic…
View article: Molecular Targets of the 5-Amido-Carboxamide Bumped Kinase Inhibitor BKI-1748 in Cryptosporidium parvum and HCT-8 Host Cells
Molecular Targets of the 5-Amido-Carboxamide Bumped Kinase Inhibitor BKI-1748 in Cryptosporidium parvum and HCT-8 Host Cells Open
Cryptosporidium parvum is an apicomplexan parasite causing persistent diarrhea in humans and animals. Issuing from target-based drug development, calcium-dependent protein kinase 1 inhibitors, collectively named bumped kinase inhibitors (B…