James A. Williams
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View article: Sensitivity and specificity of the Cobas Liat CT/NG/MG nucleic acid test in a clinical laboratory setting and point-of-care location
Sensitivity and specificity of the Cobas Liat CT/NG/MG nucleic acid test in a clinical laboratory setting and point-of-care location Open
Sexually transmitted infections, e.g., Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Mycoplasma genitalium (MG), present with similar signs/symptoms and are often treated empirically due to delays in receiving diagnostic test…
View article: Noninvasive Monitoring of Humoral Immune Responses in Men With Acute <i>Chlamydia trachomatis</i> Urethral Infection Using First-Catch Urine
Noninvasive Monitoring of Humoral Immune Responses in Men With Acute <i>Chlamydia trachomatis</i> Urethral Infection Using First-Catch Urine Open
We investigated the relationship between Chlamydia trachomatis load and anti-chlamydial antibody using first-catch urine from 108 men with nongonococcal urethritis. Men who reported prior chlamydia had elevated anti-chlamydial IgA and lowe…
View article: Structures of the Varicella Zoster Virus Glycoprotein E and Epitope Mapping of Vaccine-Elicited Antibodies
Structures of the Varicella Zoster Virus Glycoprotein E and Epitope Mapping of Vaccine-Elicited Antibodies Open
Background: Varicella zoster virus (VZV) is the causative agent for chickenpox and herpes zoster (HZ, shingles). HZ is a debilitating disease affecting elderly and immunocompromised populations. Glycoprotein E (gE) is indispensable for vir…
View article: Structural and computational design of a SARS-CoV-2 spike antigen with improved expression and immunogenicity
Structural and computational design of a SARS-CoV-2 spike antigen with improved expression and immunogenicity Open
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern challenge the efficacy of approved vaccines, emphasizing the need for updated spike antigens. Here, we use an evolutionary-based design aimed at boosting prot…
View article: Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors
Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors Open
View article: Sexual behavior shapes male genitourinary microbiome composition
Sexual behavior shapes male genitourinary microbiome composition Open
View article: Delivery of non-viral naked DNA vectors to liver in small weaned pigs by hydrodynamic retrograde intrabiliary injection
Delivery of non-viral naked DNA vectors to liver in small weaned pigs by hydrodynamic retrograde intrabiliary injection Open
View article: Sexual Behavior Shapes the Adult Male Genitourinary Microbiome
Sexual Behavior Shapes the Adult Male Genitourinary Microbiome Open
View article: Evaluation of Clinical, Gram Stain, and Microbiological Cure Outcomes in Men Receiving Azithromycin for Acute Nongonococcal Urethritis: Discordant Cures Are Associated With Mycoplasma genitalium Infection.
Evaluation of Clinical, Gram Stain, and Microbiological Cure Outcomes in Men Receiving Azithromycin for Acute Nongonococcal Urethritis: Discordant Cures Are Associated With Mycoplasma genitalium Infection. Open
In azithromycin-treated NGU, clinical cure predicts microbiological cure for all STIs, except MG. Nongonococcal urethritis management should include MG testing and confirmation of microbiological cure in azithromycin-treated MG-NGU when MR…
View article: P267 Discordant cures are associated with Mycoplasma genitalium infection in men treated with azithromycin for nongonococcal urethritis
P267 Discordant cures are associated with Mycoplasma genitalium infection in men treated with azithromycin for nongonococcal urethritis Open
Background In men with nongonococcal urethritis (NGU), clinicians and patients rely on the resolution of urethritis signs and symptoms to determine the need for additional testing/treatment and when to resume sexual activity, respectively.…
View article: Author response: Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies
Author response: Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract A prerequisite for the design…
View article: Analytical and Clinical Sample Performance Characteristics of the Onclarity Assay for the Detection of Human Papillomavirus
Analytical and Clinical Sample Performance Characteristics of the Onclarity Assay for the Detection of Human Papillomavirus Open
The objective of this study was to determine the result reproducibility and performance of the BD Onclarity human papillomavirus (HPV) assay (Onclarity) on the BD Viper LT platform using both contrived and clinical specimens. Reproducibili…
View article: Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies
Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies Open
A prerequisite for the design of an HIV vaccine that elicits protective antibodies is understanding the developmental pathways that result in desirable antibody features. The development of antibodies that mediate antibody-dependent cellul…
View article: Angiogenic and immunomodulatory biomarkers in axitinib-treated patients with advanced renal cell carcinoma
Angiogenic and immunomodulatory biomarkers in axitinib-treated patients with advanced renal cell carcinoma Open
Aim: Immunomodulatory mechanisms contributing to angiogenic inhibition in renal tumors are not well characterized. We report associations between efficacy and tumor-associated immune cells and mRNA/miRNA expression in patients from …
View article: Novel Non-integrating DNA Nano-S/MAR Vectors Restore Gene Function in Isogenic Patient-Derived Pancreatic Tumor Models
Novel Non-integrating DNA Nano-S/MAR Vectors Restore Gene Function in Isogenic Patient-Derived Pancreatic Tumor Models Open
We describe herein non-integrating minimally sized nano-S/MAR DNA vectors, which can be used to genetically modify dividing cells in place of integrating vectors. They represent a unique genetic tool, which avoids vector-mediated damage. P…
View article: Nanoplasmid Vectors Co-expressing Innate Immune Agonists Enhance DNA Vaccines for Venezuelan Equine Encephalitis Virus and Ebola Virus
Nanoplasmid Vectors Co-expressing Innate Immune Agonists Enhance DNA Vaccines for Venezuelan Equine Encephalitis Virus and Ebola Virus Open
View article: No Pathogen-Specific Sign or Symptom Predicts the Etiology of Monomicrobial Nongonococcal Urethritis in Men
No Pathogen-Specific Sign or Symptom Predicts the Etiology of Monomicrobial Nongonococcal Urethritis in Men Open
Identifying pathogen-specific signs or symptoms of nongonococcal urethritis could improve syndromic management accuracy. We evaluated nongonococcal urethritis signs and symptoms in 220 men with single-pathogen infections ( Chlamydia tracho…
View article: Aetiology and prevalence of mixed-infections and mono-infections in non-gonococcal urethritis in men: a case-control study
Aetiology and prevalence of mixed-infections and mono-infections in non-gonococcal urethritis in men: a case-control study Open
Objectives Chlamydia trachomatis (CT) and Mycoplasma genitalium (MG) cause the majority of non-gonococcal urethritis (NGU). The role of Ureaplasma urealyticum (UU) in NGU is unclear. Prior case–control studies that examined the association…
View article: P795 Prevalence and etiology of post-azithromycin persistent non-gonococcal urethritis (NGU) symptoms in men
P795 Prevalence and etiology of post-azithromycin persistent non-gonococcal urethritis (NGU) symptoms in men Open
Background Persistent NGU occurs when symptoms persist after empiric NGU treatment and has been associated with Mycoplasma genitalium (MG) infection. The prevalence and etiology of persistent NGU in men remains largely unknown. Meth…
View article: P493 Determination of<i>Chlamydia trachomatis</i>organism load in men with Non-Gonococcal Urethritis (NGU)
P493 Determination of<i>Chlamydia trachomatis</i>organism load in men with Non-Gonococcal Urethritis (NGU) Open
Background The ability to quantify the organism load of Chlamydia trachomatis (CT) using a commercial assay could expand insights from epidemiological studies. This approach can be applied to routine diagnostic testing, and multiple…
View article: P794 Signs and symptoms associated with single-pathogen nongonococcal urethritis in men
P794 Signs and symptoms associated with single-pathogen nongonococcal urethritis in men Open
Background Syndromic management remains the standard nongonococcal urethritis (NGU) treatment approach. Whether pathogen-specific signs/symptoms inform treatment decisions remains unclear. We identified men with single- and mixed-pathogen …
View article: Kappa chain maturation helps drive rapid development of an infant HIV-1 broadly neutralizing antibody lineage
Kappa chain maturation helps drive rapid development of an infant HIV-1 broadly neutralizing antibody lineage Open
View article: Detection of Rectal Chlamydia trachomatis in Heterosexual Men Who Report Cunnilingus
Detection of Rectal Chlamydia trachomatis in Heterosexual Men Who Report Cunnilingus Open
Background Rectal infection with Chlamydia trachomatis (CT) is frequent in women who deny receptive anal sex and is thought to arise from autoinoculation of the rectum from vaginal secretions. An alternate hypothesis is that oral sex inocu…
View article: 1491. <i>Mycoplasma genitalium</i>: A Concordance Study in Heterosexual Partnerships at Risk for Chlamydial Infection
1491. <i>Mycoplasma genitalium</i>: A Concordance Study in Heterosexual Partnerships at Risk for Chlamydial Infection Open
BACKGROUND: Mycoplasma genitalium (MG) causes symptomatic nonchlamydial, nongonococcal urethritis in men, and cervicitis, pelvic inflammatory disease and infertility in women. We aimed to determine: prevalence and concordance of MG infecti…
View article: Rapid development of an infant-derived HIV-1 broadly neutralizing antibody lineage
Rapid development of an infant-derived HIV-1 broadly neutralizing antibody lineage Open
Summary HIV-infected infants develop broadly neutralizing plasma responses with more rapid kinetics than adults, suggesting the ontogeny of infant responses could better inform a path to achievable vaccine targets. We developed computation…
View article: Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire
Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire Open
View article: Regional Differences in Efficacy, Safety, and Biomarkers for Second-Line Axitinib in Patients with Advanced Hepatocellular Carcinoma: From a Randomized Phase II Study
Regional Differences in Efficacy, Safety, and Biomarkers for Second-Line Axitinib in Patients with Advanced Hepatocellular Carcinoma: From a Randomized Phase II Study Open
Background: An unmet need exists for treatment of patients with advanced hepatocellular carcinoma (HCC) who progress on or are intolerant to sorafenib. A global randomized phase II trial (ClinicalTrial.gov No. NCT01210495) of axitinib, a v…
View article: Protective effect of a polyvalent influenza DNA vaccine in pigs
Protective effect of a polyvalent influenza DNA vaccine in pigs Open
View article: P1.26 Evaluation of two diagnostic systems for the detection of<i>chlamydia trachomatis</i>(CT) and<i>neisseria gonorrhoeae</i>(NG) using male urethral swabs
P1.26 Evaluation of two diagnostic systems for the detection of<i>chlamydia trachomatis</i>(CT) and<i>neisseria gonorrhoeae</i>(NG) using male urethral swabs Open
Introduction Urine is the specimen of choice for CT and NG molecular testing from males and many of the next generation testing platforms are not FDA approved for use with urethral swabs. Urethral swabs remain valuable in research settings…
View article: P3.120 Prevalence of<i>ureaplasma urealyticum</i>in urine of men attending a sexually transmitted disease clinic
P3.120 Prevalence of<i>ureaplasma urealyticum</i>in urine of men attending a sexually transmitted disease clinic Open
Introduction Ureaplasma urealyticum (UU) is probably one of the causes of non-gonococcal, non-chlamydial urethritis in men and post-partum endometritis in women. The epidemiology of UU is currently unclear because culture isolation is diff…