Andries Zijlstra
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View article: Secreted exosomes induce filopodia formation
Secreted exosomes induce filopodia formation Open
Filopodia are dynamic adhesive cytoskeletal structures that are critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. Filopodia are also critical for neuronal synapse formation. While dyna…
View article: Extracellular vesicle heterogeneity through the lens of multiomics
Extracellular vesicle heterogeneity through the lens of multiomics Open
Extracellular vesicles (EVs) are heterogeneous in size, biogenesis, content, and function. Aggressive cancer cells release a distinct, poorly characterized, and particularly large EV subtype, namely large oncosomes (LOs). This study employ…
View article: Secreted exosomes induce filopodia formation
Secreted exosomes induce filopodia formation Open
Filopodia are dynamic adhesive cytoskeletal structures that are critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. Filopodia are also critical for neuronal synapse formation. While dyna…
View article: Secreted exosomes induce filopodia formation
Secreted exosomes induce filopodia formation Open
Filopodia are dynamic adhesive cytoskeletal structures that are critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. Filopodia are also critical for neuronal synapse formation. While dyna…
View article: Extracellular Vesicles heterogeneity through the lens of multiomics
Extracellular Vesicles heterogeneity through the lens of multiomics Open
Extracellular vesicles (EVs) are heterogenous in size, biogenesis, cargo and function. Beside small EVs, aggressive tumor cells release a population of particularly large EVs, namely large oncosomes (LO). This study provides the first reso…
View article: Multiparametric Single-Vesicle Flow Cytometry Resolves Extracellular Vesicle Heterogeneity and Reveals Selective Regulation of Biogenesis and Cargo Distribution
Multiparametric Single-Vesicle Flow Cytometry Resolves Extracellular Vesicle Heterogeneity and Reveals Selective Regulation of Biogenesis and Cargo Distribution Open
Mammalian cells release a heterogeneous array of extracellular vesicles (EVs) that contribute to intercellular communication by means of the cargo that they carry. To resolve EV heterogeneity and determine if cargo is partitioned into sele…
View article: Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches Open
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have ge…
View article: P1246: CELL TYPE IDENTIFICATION USING MULTIPLEX IMMUNOFLUORESCENCE (MIF) GUIDED MACHINE LEARNING IN DLBCL
P1246: CELL TYPE IDENTIFICATION USING MULTIPLEX IMMUNOFLUORESCENCE (MIF) GUIDED MACHINE LEARNING IN DLBCL Open
Topic: 20. Lymphoma Biology & Translational Research Background: Diffuse large B-cell lymphoma (DLBCL), the most common form of Non-Hodgkin’s Lymphoma, is very aggressive and heterogeneous. Up to 40% of patients develop relapsed/refractory…
View article: Automated tumor immunophenotyping predicts clinical benefit from anti-PD-L1 immunotherapy
Automated tumor immunophenotyping predicts clinical benefit from anti-PD-L1 immunotherapy Open
Background Cancer immunotherapy has transformed the clinical approach to patients with malignancies as profound benefits can be seen in a subset of patients. To identify this subset, biomarker analyses increasingly focus on phenotypic and …
View article: Supplemental Figures S1 and S2 from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms
Supplemental Figures S1 and S2 from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms Open
Analysis all available cancer types in TCGA data sets. Correlation analysis of METABRIC data between stages or aggressiveness of cancer vs. ECM proteins expression.
View article: Data from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Data from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Abnormalities in nuclear shape are a well-known feature of cancer, but their contribution to malignant progression remains poorly understood. Here, we show that depletion of the cytoskeletal regulator, Diaphanous-related formin 3 (DIAPH3),…
View article: Movie S1 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Movie S1 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
3D image of nuclear membrane blebbing and emerin mislocalization in BT-549 DIAPH3-depleted cells. DNA (Hoechst, blue), emerin (FITC, green) and membrane (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate, Dil, red) stainin…
View article: Supplementary Figures and Table from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Supplementary Figures and Table from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Emerin deregulation links nuclear shape instability to metastatic potential
View article: Supplementary Figure legends from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Supplementary Figure legends from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Emerin deregulation links nuclear shape instability to metastatic potential
View article: Movie S3 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Movie S3 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
DU145 DIAPH3-depleted cells shed EV. Time-lapse analysis of DU145 DIAPH3-depleted cells treated with EGF and IL6 (exacerbates amoeboid features (13)) and stained with CellMask Orange. The cells were recorded over a period of 135s.
View article: Movie S2 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Movie S2 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Nuclear membrane blebbing in amoeboid cells. Time-lapse analysis of DU145 DIAPH3-depleted cells treated with EGF and IL6 (exacerbates amoeboid features (13)) and stained with lipid fluorescent dye CellMask Orange.
View article: Supplementary Figure legends from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Supplementary Figure legends from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Emerin deregulation links nuclear shape instability to metastatic potential
View article: Data from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms
Data from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms Open
TGFβ plays a crucial role in the tumor microenvironment by regulating cell–cell and cell–stroma interactions. We previously demonstrated that TGFβ signaling on myeloid cells regulates expression of CD73, a key enzyme for production of aden…
View article: Supplemental Figures S1 and S2 from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms
Supplemental Figures S1 and S2 from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms Open
Analysis all available cancer types in TCGA data sets. Correlation analysis of METABRIC data between stages or aggressiveness of cancer vs. ECM proteins expression.
View article: Data from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Data from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Abnormalities in nuclear shape are a well-known feature of cancer, but their contribution to malignant progression remains poorly understood. Here, we show that depletion of the cytoskeletal regulator, Diaphanous-related formin 3 (DIAPH3),…
View article: Data from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms
Data from Myeloid Cell–Derived TGFβ Signaling Regulates ECM Deposition in Mammary Carcinoma via Adenosine-Dependent Mechanisms Open
TGFβ plays a crucial role in the tumor microenvironment by regulating cell–cell and cell–stroma interactions. We previously demonstrated that TGFβ signaling on myeloid cells regulates expression of CD73, a key enzyme for production of aden…
View article: Movie S3 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Movie S3 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
DU145 DIAPH3-depleted cells shed EV. Time-lapse analysis of DU145 DIAPH3-depleted cells treated with EGF and IL6 (exacerbates amoeboid features (13)) and stained with CellMask Orange. The cells were recorded over a period of 135s.
View article: Supplementary Figures and Table from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Supplementary Figures and Table from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Emerin deregulation links nuclear shape instability to metastatic potential
View article: Movie S1 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Movie S1 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
3D image of nuclear membrane blebbing and emerin mislocalization in BT-549 DIAPH3-depleted cells. DNA (Hoechst, blue), emerin (FITC, green) and membrane (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate, Dil, red) stainin…
View article: Movie S2 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential
Movie S2 from Emerin Deregulation Links Nuclear Shape Instability to Metastatic Potential Open
Nuclear membrane blebbing in amoeboid cells. Time-lapse analysis of DU145 DIAPH3-depleted cells treated with EGF and IL6 (exacerbates amoeboid features (13)) and stained with lipid fluorescent dye CellMask Orange.
View article: Supplementary Figure 3 from Elevated ALCAM Shedding in Colorectal Cancer Correlates with Poor Patient Outcome
Supplementary Figure 3 from Elevated ALCAM Shedding in Colorectal Cancer Correlates with Poor Patient Outcome Open
Supplementary Figure 3 PDF file 71K,Supplemental Figure 3: Lack of cytoplasmic ALCAM corresponds with poor patient outcome
View article: Supplementary Figures 1 through 9 from ALCAM/CD166 Is a TGF-β–Responsive Marker and Functional Regulator of Prostate Cancer Metastasis to Bone
Supplementary Figures 1 through 9 from ALCAM/CD166 Is a TGF-β–Responsive Marker and Functional Regulator of Prostate Cancer Metastasis to Bone Open
PDF - 1790K, ALCAM expression is upregulated in response to the loss of ECadherin (S1); TGFbeta-induced shedding (S2); ALCAM serum half-life in mice (S3); ALCAM shedding by prostate and breast cancer cells in vitro and in vivo (S4); Compou…