Andrew J. Newman
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View article: Linear embeddings of random complexes
Linear embeddings of random complexes Open
For $X \sim X(n; 1, n^{-α_1}, n^{-α_2}, ...)$ in the multiparameter random simplicial complex model we establish necessary and sufficient strict inequalities on the $α_i$'s to linearly embed the complex into $\mathbb{R}^{2d}$.
View article: psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation Open
RNA helicases remodel the spliceosome to enable pre-mRNA splicing, but their binding and mechanism of action remain poorly understood. To define helicase-RNA contacts in specific spliceosomal states, we develop purified spliceosome iCLIP (…
View article: PsiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
PsiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation Open
Eight RNA helicases remodel the spliceosome to effect pre-mRNA splicing but their mechanism of action remains poorly understood. We have developed “purified spliceosome iCLIP” (psiCLIP) to define helicase-RNA contacts in specific spliceoso…
View article: Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast
Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast Open
Noncoding introns are removed from nuclear precursor messenger RNA (pre-mRNA) in a two-step phosphoryl transfer reaction by the spliceosome, a dynamic multimegadalton enzyme. Cryo-electron microscopy (cryo-EM) structures of the Saccharomyc…
View article: A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation
A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation Open
A human P spliceosome structure Splicing of some pre–messenger RNAs could be regulated by cell type–specific splicing factors. Fica et al. describe the cryo–electron microscopy structure of the human postcatalytic (P) spliceosome. Surprisi…
View article: Dynamic protein–RNA interactions in mediating splicing catalysis
Dynamic protein–RNA interactions in mediating splicing catalysis Open
The spliceosome is assembled via sequential interactions of pre-mRNA with five small nuclear RNAs and many proteins. Recent determination of cryo-EM structures for several spliceosomal complexes has provided deep insights into interactions…
View article: Molecular Mechanism and Evolution of Nuclear Pre-mRNA and Group II Intron Splicing: Insights from Cryo-Electron Microscopy Structures
Molecular Mechanism and Evolution of Nuclear Pre-mRNA and Group II Intron Splicing: Insights from Cryo-Electron Microscopy Structures Open
Nuclear pre-mRNA splicing and group II intron self-splicing both proceed by two-step transesterification reactions via a lariat intron intermediate. Recently determined cryo-electron microscopy (cryo-EM) structures of catalytically active …
View article: Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection
Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection Open
Understanding splicing from the 3′ end The spliceosome removes introns from eukaryotic mRNA precursors and yields mature transcripts by joining exons. Despite decades of functional studies and recent progress in understanding the spliceoso…
View article: Post-catalytic spliceosome structure reveals mechanism of 3'-splice site selection
Post-catalytic spliceosome structure reveals mechanism of 3'-splice site selection Open
Introns are removed from eukaryotic mRNA precursors by the spliceosome in two transesterification reactions – branching and exon ligation. Following branching, the 5'-exon remains paired to U5 snRNA loop 1, but the mechanism of 3'-splice s…
View article: Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain
Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain Open
Spliceosomal proteins Hsh49p and Cus1p are components of SF3b, which together with SF3a, Msl1p/Lea1p, Sm proteins, and U2 snRNA, form U2 snRNP, which plays a crucial role in pre-mRNA splicing. Hsh49p, comprising two RRMs, forms a heterodim…
View article: The regulation of L-selectin activity by proteolysis
The regulation of L-selectin activity by proteolysis Open
L-selectin (CD62L) is a type I transmembrane protein expressed by lymphocytes which directs their migration from the bloodstream into lymph nodes and infected tissues.
\nStimulation of the T cell receptor (TCR) activates the enzyme A Disin…