Angeliki Moutsiopoulou
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View article: Development and Validation of a High‐Throughput Screening Assay for the Legionella ADP‐Ribosyl Transferase SdeA
Development and Validation of a High‐Throughput Screening Assay for the Legionella ADP‐Ribosyl Transferase SdeA Open
Ubiquitination of proteins is one of the most crucial post‐translational modifications in eukaryotic cells, typically involving conjugation of ubiquitin to a lysine residue in a substrate using a three‐enzyme cascade that relies on ATP as …
View article: Covalent Probes To Capture <i>Legionella pneumophila</i> Dup Effector Enzymes
Covalent Probes To Capture <i>Legionella pneumophila</i> Dup Effector Enzymes Open
Upon infection of host cells, Legionella pneumophila releases a multitude of effector enzymes into the cell's cytoplasm that hijack a plethora of cellular activities, including the host ubiquitination pathways. Effectors belonging t…
View article: Development of covalent probes to capture<i>Legionella pneumophila</i>effector enzymes
Development of covalent probes to capture<i>Legionella pneumophila</i>effector enzymes Open
Upon infection of host cells, Legionella pneumophila releases a multitude of effector enzymes into the cells cytoplasm that hijack a plethora of cellular activities, including the hosts ubiquitination pathways. Effectors belonging to the S…
View article: Neutron-encoded diubiquitins to profile linkage selectivity of deubiquitinating enzymes
Neutron-encoded diubiquitins to profile linkage selectivity of deubiquitinating enzymes Open
Deubiquitinating enzymes are key regulators in the ubiquitin system and an emerging class of drug targets. These proteases disassemble polyubiquitin chains and many deubiquitinases show selectivity for specific polyubiquitin linkages. Howe…
View article: Arginine ADP-Ribosylation: Chemical Synthesis of Post-Translationally Modified Ubiquitin Proteins
Arginine ADP-Ribosylation: Chemical Synthesis of Post-Translationally Modified Ubiquitin Proteins Open
We describe the development and optimization of a methodology to prepare peptides and proteins modified on the arginine residue with an adenosine-di-phosphate-ribosyl (ADPr) group. Our method comprises reacting an ornithine containing poly…
View article: Bioluminescent Protein–Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System
Bioluminescent Protein–Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System Open
Although bioluminescent molecular beacons designed around resonance quenchers have shown higher signal-to-noise ratios and increased sensitivity compared with fluorescent beacon systems, bioluminescence quenching is still comparatively ine…
View article: Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics
Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics Open
The ability to monitor types, concentrations, and activities of different biomolecules is essential to obtain information about the molecular processes within cells. Successful monitoring requires a sensitive and selective tool that can re…
View article: The Effect of NSAID Pretreatment on Aqueous Humor Prostaglandin E<sub>2</sub> Concentration in Eyes Undergoing Femtosecond Laser-Assisted Capsulotomy
The Effect of NSAID Pretreatment on Aqueous Humor Prostaglandin E<sub>2</sub> Concentration in Eyes Undergoing Femtosecond Laser-Assisted Capsulotomy Open
Purpose . To assess aqueous humor concentration of prostaglandin E 2 (PGE 2 ) after capsulotomy creation using a femtosecond laser (FLAC) in patients pretreated with short-term topical ketorolac versus patients without pretreatment. Method…
View article: Design of Gaussia luciferase-based bioluminescent stem-loop probe for sensitive detection of HIV-1 nucleic acids
Design of Gaussia luciferase-based bioluminescent stem-loop probe for sensitive detection of HIV-1 nucleic acids Open
Sensitive detection of HIV-1 nucleic acids using Gaussia luciferase based bioluminescent stem-loop assay.
View article: Bioorthogonal Protein Conjugation: Application to the Development of a Highly Sensitive Bioluminescent Immunoassay for the Detection of Interferon-γ
Bioorthogonal Protein Conjugation: Application to the Development of a Highly Sensitive Bioluminescent Immunoassay for the Detection of Interferon-γ Open
Bioorthogonal conjugation eliminates the shortcomings of classical conjugation methods. The conjugation of antibodies to reporter proteins, such as bioluminescent protein, can be controlled with orthogonal conjugation methods. Here we repo…
View article: Bioorthogonal Protein Conjugation: Application for the Development of a Highly Sensitive Bioluminescent Immunoassay for the Detection of Interferon‐γ
Bioorthogonal Protein Conjugation: Application for the Development of a Highly Sensitive Bioluminescent Immunoassay for the Detection of Interferon‐γ Open
Interferon‐γ (IFN‐γ) is a 144‐amino acid long N‐terminal methionylated recombinant protein and is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally came to attention due to its anti…
View article: Expression of a soluble truncated Vargula luciferase in Escherichia coli
Expression of a soluble truncated Vargula luciferase in Escherichia coli Open
View article: Truncated Variants of Gaussia Luciferase with Tyrosine Linker for Site-Specific Bioconjugate Applications
Truncated Variants of Gaussia Luciferase with Tyrosine Linker for Site-Specific Bioconjugate Applications Open
Gaussia luciferase (Gluc)—with its many favorable traits such as small size, bright emission and exceptional stability—has become a prominent reporter protein for a wide range of bioluminescence-based detection applications. The ten intern…