Anke Busch
YOU?
Author Swipe
View article: Dissecting the RNA binding capacity of the multi-RRM protein Rrm4 essential for endosomal mRNA transport
Dissecting the RNA binding capacity of the multi-RRM protein Rrm4 essential for endosomal mRNA transport Open
RNA-binding proteins (RBPs) utilize multiple RNA-binding domains (RBDs) to engage with extensive mRNA networks. Understanding the intricate interplay of modular RBDs is essential for uncovering RBP function. Yet, how individual RBDs shape …
View article: Microbial iCLIP2: enhanced mapping of RNA–protein interaction by promoting protein and RNA stability
Microbial iCLIP2: enhanced mapping of RNA–protein interaction by promoting protein and RNA stability Open
The entire RNA life cycle, spanning from transcription to decay, is intricately regulated by RNA-binding proteins (RBPs). To understand their precise functions, it is crucial to identify direct targets, pinpoint their exact binding sites, …
View article: m6A sites in the coding region trigger translation-dependent mRNA decay
m6A sites in the coding region trigger translation-dependent mRNA decay Open
N6-Methyladenosine (m6A) is the predominant internal RNA modification in eukaryotic messenger RNAs (mRNAs) and plays a crucial role in mRNA stability. Here, using human cells, we reveal that m6A sites in the coding sequence (CDS) trigger C…
View article: Microbial iCLIP2: Enhanced mapping of RNA-Protein interaction by promoting protein and RNA stability
Microbial iCLIP2: Enhanced mapping of RNA-Protein interaction by promoting protein and RNA stability Open
The entire RNA lifecycle, spanning from transcription to decay, is intricately regulated by RNA-binding proteins (RBPs). To understand their precise functions, it is crucial to identify direct targets, pinpoint their exact binding sites, a…
View article: FUBP1 is a general splicing factor facilitating 3′ splice site recognition and splicing of long introns
FUBP1 is a general splicing factor facilitating 3′ splice site recognition and splicing of long introns Open
Splicing of pre-mRNAs critically contributes to gene regulation and proteome expansion in eukaryotes, but our understanding of the recognition and pairing of splice sites during spliceosome assembly lacks detail. Here, we identify the mult…
View article: RNA stability controlled by m6A methylation contributes to X-to-autosome dosage compensation in mammals
RNA stability controlled by m6A methylation contributes to X-to-autosome dosage compensation in mammals Open
In mammals, X-chromosomal genes are expressed from a single copy since males (XY) possess a single X chromosome, while females (XX) undergo X inactivation. To compensate for this reduction in dosage compared with two active copies of autos…
View article: TERRA increases at short telomeres in yeast survivors and regulates survivor associated senescence (SAS)
TERRA increases at short telomeres in yeast survivors and regulates survivor associated senescence (SAS) Open
Cancer cells achieve immortality by employing either homology-directed repair (HDR) or the telomerase enzyme to maintain telomeres. ALT (alternative lengthening of telomeres) refers to the subset of cancer cells that employ HDR. Many ALT f…
View article: TERRA increases at short telomeres in yeast survivors and regulates survivor associated senescence (SAS)
TERRA increases at short telomeres in yeast survivors and regulates survivor associated senescence (SAS) Open
Cancer cells achieve immortality by employing either homology-directed repair (HDR) or the telomerase enzyme to maintain telomeres. ALT (alternative lengthening of telomeres) refers to the subset of cancer cells that employ HDR. ALT mechan…
View article: High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling <i>CD19</i> splicing and CART-19 therapy resistance
High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling <i>CD19</i> splicing and CART-19 therapy resistance Open
During CART-19 immunotherapy for B-cell acute lymphoblastic leukaemia (B-ALL), many patients relapse due to loss of the cognate CD19 epitope. Since epitope loss can be caused by aberrant CD19 exon 2 processing, we herein investigate the re…
View article: Deep and accurate detection of m6A RNA modifications using miCLIP2 and m6Aboost machine learning
Deep and accurate detection of m6A RNA modifications using miCLIP2 and m6Aboost machine learning Open
N6-methyladenosine (m6A) is the most abundant internal RNA modification in eukaryotic mRNAs and influences many aspects of RNA processing. miCLIP (m6A individual-nucleotide resolution UV crosslinking and immunoprecipitation) is an antibody…
View article: The RNA-binding profile of the splicing factor SRSF6 in immortalized human pancreatic β-cells
The RNA-binding profile of the splicing factor SRSF6 in immortalized human pancreatic β-cells Open
In pancreatic β-cells, the expression of the splicing factor SRSF6 is regulated by GLIS3, a transcription factor encoded by a diabetes susceptibility gene. SRSF6 down-regulation promotes β-cell demise through splicing dysregulation of cent…
View article: Deep and accurate detection of m<sup>6</sup>A RNA modifications using miCLIP2 and m6Aboost machine learning
Deep and accurate detection of m<sup>6</sup>A RNA modifications using miCLIP2 and m6Aboost machine learning Open
N6-methyladenosine (m 6 A) is the most abundant internal RNA modification in eukaryotic mRNAs and influences many aspects of RNA processing. miCLIP (m 6 A individual-nucleotide resolution UV crosslinking and immunoprecipitation) is an anti…
View article: Checkpoint adaptation in recombination-deficient cells drives aneuploidy and resistance to genotoxic agents
Checkpoint adaptation in recombination-deficient cells drives aneuploidy and resistance to genotoxic agents Open
Human cancers frequently harbour mutations in DNA repair genes, rendering the use of DNA damaging agents as an effective therapeutic intervention. As therapy-resistant cells often arise, it is important to better understand the molecular p…
View article: An autoinhibitory intramolecular interaction proof-reads RNA recognition by the essential splicing factor U2AF2
An autoinhibitory intramolecular interaction proof-reads RNA recognition by the essential splicing factor U2AF2 Open
The recognition of cis -regulatory RNA motifs in human transcripts by RNA binding proteins (RBPs) is essential for gene regulation. The molecular features that determine RBP specificity are often poorly understood. Here, we combined NMR st…
View article: The m<sup>6</sup>A reader Ythdf restricts axonal growth in<i>Drosophila</i>through target selection modulation of the Fragile X mental retardation protein
The m<sup>6</sup>A reader Ythdf restricts axonal growth in<i>Drosophila</i>through target selection modulation of the Fragile X mental retardation protein Open
N6-methyladenosine (m 6 A) regulates a variety of physiological processes through modulation of RNA metabolism. The modification is particularly enriched in the nervous system of several species, and its dysregulation has been associated w…
View article: Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation
Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation Open
Makorins are evolutionary conserved proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila, maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained unso…
View article: Improved library preparation with the new iCLIP2 protocol
Improved library preparation with the new iCLIP2 protocol Open
Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) is a state-of-the-art technology to map the RNA interaction sites of an RNA-binding protein (RBP) across the transcriptome. Here, we present the new iCLIP2 pr…
View article: Pancreatic pericytes originate from the embryonic pancreatic mesenchyme
Pancreatic pericytes originate from the embryonic pancreatic mesenchyme Open
The embryonic origin of pericytes is heterogeneous, both between and within organs. While pericytes of coelomic organs were proposed to differentiate from the mesothelium, a single-layer squamous epithelium, the embryonic origin of pancrea…
View article: MOESM4 of The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation
MOESM4 of The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation Open
Additional file 4: Table S3. with MaxQuant analysis of MS data from the ubiquitin remnant profiling from MKRN1 KD2 in HEK293T cells. (XLSX 7940 kb)
View article: MOESM5 of The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation
MOESM5 of The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation Open
Additional file 5: Table S4. with MaxQuant analysis of MS data from the proteome analysis from MKRN1 KD2 in HEK293T cells. (XLSX 2270 kb)
View article: MOESM2 of The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation
MOESM2 of The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation Open
Additional file 2: Table S1. with MaxQuant analysis of MS data from the SILAC-AP for GFP-MKRN1wt, GFP-MKRN1PAM2mut, and GFP-MKRN1RINGmut. (XLSX 1409 kb)
View article: Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of<i>oskar</i>translation
Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of<i>oskar</i>translation Open
Makorins are evolutionary conserved proteins that contain C 3 H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained uns…