Ann E. Blechl
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Small serine recombination systems ParA‐<i><span>MRS</span></i> and CinH‐<i><span>RS</span>2</i> perform precise excision of plastid <span>DNA</span> Open
Selectable marker genes ( SMG s) are necessary for selection of transgenic plants. However, once stable transformants have been identified, the marker gene is no longer needed. In this study, we demonstrate the use of the small serine reco…
Accurate measurement of transgene copy number in crop plants using droplet digital <span>PCR</span> Open
Summary Genetic transformation is a powerful means for the improvement of crop plants, but requires labor‐ and resource‐intensive methods. An efficient method for identifying single‐copy transgene insertion events from a population of inde…
PvPGIP2 Accumulation in Specific Floral Tissues But Not in the Endosperm Limits <i>Fusarium graminearum</i> Infection in Wheat Open
Fusarium head blight (FHB) caused by Fusarium graminearum is one of the most destructive fungal diseases of wheat worldwide. The pathogen infects the spike at flowering time and causes severe yield losses, deterioration of grain quality, a…
Advancing Crop Transformation in the Era of Genome Editing Open
Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technologica…