Michelle A. Emrick
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View article: Quantification of glucagon and oxyntomodulin by protein precipitation-immunoaffinity enrichment-LC-MS/MS
Quantification of glucagon and oxyntomodulin by protein precipitation-immunoaffinity enrichment-LC-MS/MS Open
We have validated a method with a highly detailed standard operating procedure. We have characterized calibration materials to help maintain accuracy and achieve between-day and between-laboratory harmonization. The assay will be beneficia…
View article: Interlaboratory Comparison of Antibody-Free LC-MS/MS Measurements of C-peptide and Insulin
Interlaboratory Comparison of Antibody-Free LC-MS/MS Measurements of C-peptide and Insulin Open
Background The enhanced precision and selectivity of liquid chromatography–tandem mass spectrometry (LC-MS/MS) makes it an attractive alternative to certain clinical immunoassays. Easily transferrable work flows could help facilitate harmo…
View article: High doses of exogenous glucagon stimulate insulin secretion and reduce insulin clearance in healthy humans
High doses of exogenous glucagon stimulate insulin secretion and reduce insulin clearance in healthy humans Open
Abstract (186 words)Glucagon is generally defined as a counter-regulatory hormone with a primary role to raise blood glucose concentrations by increasing endogenous glucose production (EGP) in response to hypoglycemia. However, glucagon ha…
View article: High Doses of Exogenous Glucagon Stimulate Insulin Secretion and Reduce Insulin Clearance in Healthy Humans
High Doses of Exogenous Glucagon Stimulate Insulin Secretion and Reduce Insulin Clearance in Healthy Humans Open
Glucagon is generally defined as a counterregulatory hormone with a primary role to raise blood glucose concentrations by increasing endogenous glucose production (EGP) in response to hypoglycemia. However, glucagon has long been known to …
View article: High doses of exogenous glucagon stimulate insulin secretion and reduce insulin clearance in healthy humans
High doses of exogenous glucagon stimulate insulin secretion and reduce insulin clearance in healthy humans Open
Abstract (186 words)Glucagon is generally defined as a counter-regulatory hormone with a primary role to raise blood glucose concentrations by increasing endogenous glucose production (EGP) in response to hypoglycemia. However, glucagon ha…
View article: Metrologically Traceable Quantification of 3 Apolipoprotein E Isoforms in Cerebrospinal Fluid
Metrologically Traceable Quantification of 3 Apolipoprotein E Isoforms in Cerebrospinal Fluid Open
Background APOE genotype is associated with Alzheimer disease. Thus, the concentration of apolipoprotein E (apoE) isoforms in cerebrospinal fluid (CSF) could be altered in dementia. However, conflicting results have been obtained in differ…
View article: A distributable LC-MS/MS method for the measurement of serum thyroglobulin
A distributable LC-MS/MS method for the measurement of serum thyroglobulin Open
Sensitive mass spectrometry-based thyroglobulin measurement can be achieved using a monoclonal antibody during peptide immunoaffinity purification and the addition of mobile phase DMSO. Laboratories interested in deploying this assay can u…
View article: A Targeted Liquid Chromatography–Tandem Mass Spectrometry Method for Simultaneous Quantification of Peptides from the Carboxyl-terminal Region of Type III Procollagen, Biomarkers of Collagen Turnover
A Targeted Liquid Chromatography–Tandem Mass Spectrometry Method for Simultaneous Quantification of Peptides from the Carboxyl-terminal Region of Type III Procollagen, Biomarkers of Collagen Turnover Open
Background The development of analytical approaches to help reduce the risk of growth hormone (GH) doping is important to fair competition and the health of athletes. However, the reliable detection of GH use remains challenging. The ident…
View article: Multiplexed quantification of insulin and C-peptide by LC-MS/MS without the use of antibodies
Multiplexed quantification of insulin and C-peptide by LC-MS/MS without the use of antibodies Open
A novel LC-MS/MS method for the simultaneous analysis of insulin and C-peptide using Glu-C digestion was developed and evaluated. A detailed standard operating procedure is provided to help facilitate implementation in other laboratories.
View article: Tandem Mass Spectrometry–Based Amyloid Typing Using Manual Microdissection and Open-Source Data Processing
Tandem Mass Spectrometry–Based Amyloid Typing Using Manual Microdissection and Open-Source Data Processing Open
Objectives Standard implementations of amyloid typing by liquid chromatography–tandem mass spectrometry use capabilities unavailable to most clinical laboratories. To improve accessibility of this testing, we explored easier approaches to …