Olivia Steele‐Mortimer
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View article: HilD-regulated chemotaxis proteins contribute to <i>Salmonella</i> Typhimurium colonization in the gut
HilD-regulated chemotaxis proteins contribute to <i>Salmonella</i> Typhimurium colonization in the gut Open
In the enteric pathogen Salmonella Typhimurium, invasion and motility are coordinated by HilD, a master regulator that activates expression of genes encoding the type III secretion system 1 and some motility genes, including the chemotaxis…
View article: IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium
IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium Open
Mucosal delivery of IL-27 has been shown to have a therapeutic benefit in murine models of inflammatory bowel disease (IBD). The IL-27 effect was associated with phosphorylated STAT1 (pSTAT1), a product of IL27 receptor signaling, in bowel…
View article: Correction: Type I IFNs facilitate innate immune control of the opportunistic bacteria Burkholderia cenocepacia in the macrophage cytosol
Correction: Type I IFNs facilitate innate immune control of the opportunistic bacteria Burkholderia cenocepacia in the macrophage cytosol Open
[This corrects the article DOI: 10.1371/journal.ppat.1009395.].
View article: Global mapping of Salmonella enterica-host protein-protein interactions during infection
Global mapping of Salmonella enterica-host protein-protein interactions during infection Open
Intracellular bacterial pathogens inject effector proteins to hijack host cellular processes and promote their survival and proliferation. To systematically map effector-host protein-protein interactions (PPIs) during infection, we generat…
View article: Type I IFNs facilitate innate immune control of the opportunistic bacteria Burkholderia cenocepacia in the macrophage cytosol
Type I IFNs facilitate innate immune control of the opportunistic bacteria Burkholderia cenocepacia in the macrophage cytosol Open
The mammalian immune system is constantly challenged by signals from both pathogenic and non-pathogenic microbes. Many of these non-pathogenic microbes have pathogenic potential if the immune system is compromised. The importance of type I…
View article: Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC
Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC Open
In the enteric pathogen Salmonella enterica serovar Typhimurium, invasion and motility are coordinated by the master regulator HilD, which induces expression of the type III secretion system 1 (T3SS1) and motility genes. Methyl-accepting c…
View article: Global mapping of<i>Salmonella enterica</i>-host protein-protein interactions during infection
Global mapping of<i>Salmonella enterica</i>-host protein-protein interactions during infection Open
Summary Intracellular bacterial pathogens inject effector proteins into host cells to hijack diverse cellular processes and promote their survival and proliferation. To systematically map effector-host protein-protein interactions (PPIs) d…
View article: Foodborne infection of mice with Salmonella Typhimurium
Foodborne infection of mice with Salmonella Typhimurium Open
The bacterial pathogen Salmonella enterica serovar Typhimurium is one of the most common causes of foodborne disease in humans and is also an important model system for bacterial pathogenesis. Oral inoculation of C57Bl/6 mice, which are ge…
View article: A role for the <i>Salmonella</i> Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells
A role for the <i>Salmonella</i> Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells Open
Summary Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that invades the intestinal epithelium. Following invasion of epithelial cells, Salmonella survives and replicates within two distinct intracellular ni…
View article: Highly reproducible murine model of oral<i>Salmonella</i>infection via inoculated food
Highly reproducible murine model of oral<i>Salmonella</i>infection via inoculated food Open
Oral infection of mice with Salmonella Typhimurium is an important model system. In particular C57Bl/6 mice, which are susceptible to Salmonella , are used to study both systemic and gastrointestinal pathogenesis. Pretreatment with strepto…
View article: The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium
The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium Open
THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the…
View article: Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils Open
A long-standing notion is that IAV inhibits normal neutrophil function and thereby predisposes individuals to secondary bacterial infections. Here we report that seasonal H1N1 IAV primes human neutrophils for enhanced killing of Staphyloco…
View article: Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions
Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions Open
Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters …
View article: A second wave of Salmonella T3SS1 activity prolongs the lifespan of infected epithelial cells
A second wave of Salmonella T3SS1 activity prolongs the lifespan of infected epithelial cells Open
Type III secretion system 1 (T3SS1) is used by the enteropathogen Salmonella enterica serovar Typhimurium to establish infection in the gut. Effector proteins translocated by this system across the plasma membrane facilitate invasion of in…
View article: Genetic Determinants of Salmonella enterica Serovar Typhimurium Proliferation in the Cytosol of Epithelial Cells
Genetic Determinants of Salmonella enterica Serovar Typhimurium Proliferation in the Cytosol of Epithelial Cells Open
Intestinal epithelial cells provide an important colonization niche for Salmonella enterica serovar Typhimurium during gastrointestinal infections. In infected epithelial cells, a subpopulation of S . Typhimurium bacteria damage their inte…
View article: Inherent Variability of Growth Media Impacts the Ability of Salmonella Typhimurium to Interact with Host Cells
Inherent Variability of Growth Media Impacts the Ability of Salmonella Typhimurium to Interact with Host Cells Open
Efficient invasion of non-phagocytic cells, such as intestinal epithelial cells, by Salmonella Typhimurium is dependent on the Salmonella Pathogenicity Island 1 (SPI-1)-encoded Type Three Secretion System. The environmental cues involved i…
View article: Replication of Salmonella enterica Serovar Typhimurium in Human Monocyte-Derived Macrophages
Replication of Salmonella enterica Serovar Typhimurium in Human Monocyte-Derived Macrophages Open
Salmonella enterica serovar Typhimurium is a common cause of food-borne gastrointestinal illness, but additionally it causes potentially fatal bacteremia in some immunocompromised patients. In mice, systemic spread and replication of the b…