Anna E. Eastman
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View article: Beyond polyA: scalable single-cell total RNA-seq unifies coding and non-coding transcriptomics
Beyond polyA: scalable single-cell total RNA-seq unifies coding and non-coding transcriptomics Open
Non-coding RNAs represent a widespread and diverse layer of post-transcriptional regulation across cell types and states, yet much of their diversity remains uncharted at single-cell resolution. This gap stems from the limitations of widel…
View article: Modeling Glioma Intratumoral Heterogeneity with Primary Human Neural Stem and Progenitor Cells
Modeling Glioma Intratumoral Heterogeneity with Primary Human Neural Stem and Progenitor Cells Open
Glioblastoma multiforme (GBM) is a deadly form of glioma notable for its significant intratumoral heterogeneity, which is believed to drive therapy resistance. GBM has been observed to mimic a neural stem cell hierarchy reminiscent of norm…
View article: Purification and characterization of human neural stem and progenitor cells
Purification and characterization of human neural stem and progenitor cells Open
The human brain undergoes rapid development at mid-gestation from a pool of neural stem and progenitor cells (NSPCs) that give rise to the neurons, oligodendrocytes, and astrocytes of the mature brain. Functional study of these cell types …
View article: EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis
EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis Open
The erythroid terminal differentiation program couples sequential cell divisions with progressive reductions in cell size. The erythropoietin receptor (EpoR) is essential for erythroblast survival, but its other functions are not well char…
View article: The Erythropoietin Receptor Stimulates Rapid Cycling and Formation of Larger Red Cells During Mouse and Human Erythropoiesis
The Erythropoietin Receptor Stimulates Rapid Cycling and Formation of Larger Red Cells During Mouse and Human Erythropoiesis Open
Erythroid terminal differentiation entails cell divisions that are coupled to progressive decreases in cell size. EpoR signaling is essential for the survival of erythroid precursors, but it is unclear whether it has other functions in the…
View article: Reprogramming progressive cells display low CAG promoter activity
Reprogramming progressive cells display low CAG promoter activity Open
There is wide variability in the propensity of somatic cells to reprogram into pluripotency in response to the Yamanaka factors. How to segregate these variabilities to enrich for cells of specific traits that reprogram efficiently remains…
View article: Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter
Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter Open
Cell proliferation changes concomitantly with fate transitions during reprogramming, differentiation, regeneration, and oncogenesis. Methods to resolve cell cycle length heterogeneity in real time are currently lacking. Here, we describe a…
View article: The palette of techniques for cell cycle analysis
The palette of techniques for cell cycle analysis Open
The cell division cycle is the generational period of cellular growth and propagation. Cell cycle progression needs to be highly regulated to preserve genomic fidelity while increasing cell number. In multicellular organisms, the cell cycl…
View article: YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells
YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells Open
Yes-associated protein (YAP) is known to promote the stemness of multiple stem cell types, including pluripotent stem cells, while also antagonizing pluripotency during early embryogenesis. How YAP accomplishes these distinct functions rem…
View article: Reprogramming progressive cells display low CAG promoter activity
Reprogramming progressive cells display low CAG promoter activity Open
There is wide variability in the propensity of somatic cells to reprogram into pluripotency in response to the Yamanaka factors. How to segregate these variability to enrich for cells of specific traits that reprogram efficiently remains c…
View article: Publisher Correction: MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors
Publisher Correction: MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors Open
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
View article: Cell cycle dynamics in the reprogramming of cellular identity
Cell cycle dynamics in the reprogramming of cellular identity Open
Reprogramming of cellular identity is fundamentally at odds with replication of the genome: cell fate reprogramming requires complex multidimensional epigenomic changes, whereas genome replication demands fidelity. In this review, we discu…
View article: Resolving cell cycle speed in one snapshot with a live-cell fluorescent reporter
Resolving cell cycle speed in one snapshot with a live-cell fluorescent reporter Open
Summary Cell proliferation changes concomitantly with fate transitions during reprogramming, differentiation, regeneration, and oncogenesis. Methods to resolve cell cycle length heterogeneity in real-time are currently lacking. Here, we de…
View article: MLL-AF9 Initiates Transformation From Fast-Proliferating Myeloid Progenitors
MLL-AF9 Initiates Transformation From Fast-Proliferating Myeloid Progenitors Open
Cancer is a hyper-proliferative clonal disease. Whether the proliferative state originates from the cell-of-origin or emerges later remains elusive. By tracking de novo transformation from normal hematopoietic progenitors expressing an acu…
View article: Non-cell-autonomous promotion of pluripotency induction mediated by YAP
Non-cell-autonomous promotion of pluripotency induction mediated by YAP Open
SUMMARY While Yes-associated protein (YAP) antagonizes pluripotency during early embryogenesis, it has also been shown to promote stemness of multiple stem cell types, including pluripotent stem cells. Whether cellular context underlies th…