Anna E. Romano
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View article: Mesoscale eddies shape <i>Prochlorococcus</i> community structure and dynamics in the oligotrophic open ocean
Mesoscale eddies shape <i>Prochlorococcus</i> community structure and dynamics in the oligotrophic open ocean Open
Mesoscale eddies, horizontally rotating currents sometimes referred to as “ocean weather,” influence open ocean macronutrient distributions, primary production, and microbial community structure. Such eddies impact ecosystems like the Nort…
View article: The ratio between SARS-CoV-2 RNA viral load and culturable viral titer differs depending on stage of infection
The ratio between SARS-CoV-2 RNA viral load and culturable viral titer differs depending on stage of infection Open
Analysis of incident, longitudinal RNA viral loads in saliva and nasal swabs and culturable viral titers in nasal swabs collected twice-daily by a tricenarian male infected with SARS-CoV-2 revealed the ratio between viral load and viral ti…
View article: Daily SARS-CoV-2 Nasal Antigen Tests Miss Infected and Presumably Infectious People Due to Viral Load Differences among Specimen Types
Daily SARS-CoV-2 Nasal Antigen Tests Miss Infected and Presumably Infectious People Due to Viral Load Differences among Specimen Types Open
We reveal three findings from a longitudinal study of daily nasal antigen rapid diagnostic test (Ag-RDT) evaluated against SARS-CoV-2 viral load quantification in three specimen types (saliva, nasal swab, and throat swab) in participants e…
View article: Extreme differences in SARS-CoV-2 viral loads among respiratory specimen types during presumed pre-infectious and infectious periods
Extreme differences in SARS-CoV-2 viral loads among respiratory specimen types during presumed pre-infectious and infectious periods Open
SARS-CoV-2 viral-load measurements from a single-specimen type are used to establish diagnostic strategies, interpret clinical-trial results for vaccines and therapeutics, model viral transmission, and understand virus–host interactions. H…
View article: Laboratory Evaluation Links Some False-Positive COVID-19 Antigen Test Results Observed in a Field Study to a Specific Lot of Test Strips
Laboratory Evaluation Links Some False-Positive COVID-19 Antigen Test Results Observed in a Field Study to a Specific Lot of Test Strips Open
During a household-transmission field study using COVID-19 antigen rapid diagnostic tests (Ag-RDT), a common test strip lot was identified among 3 participants with false-positive results. In blinded laboratory evaluation, this lot exhibit…
View article: Morning SARS-CoV-2 Testing Yields Better Detection of Infection Due to Higher Viral Loads in Saliva and Nasal Swabs upon Waking
Morning SARS-CoV-2 Testing Yields Better Detection of Infection Due to Higher Viral Loads in Saliva and Nasal Swabs upon Waking Open
Our findings suggest that collecting saliva and nasal swab specimens in the morning immediately after waking yields higher SARS-CoV-2 viral loads than collection later in the day. The higher viral loads from morning specimen collection are…
View article: Extreme differences in SARS-CoV-2 viral loads among respiratory specimen types during presumed pre-infectious and infectious periods
Extreme differences in SARS-CoV-2 viral loads among respiratory specimen types during presumed pre-infectious and infectious periods Open
SARS-CoV-2 viral load measurements from a single specimen type are used to establish diagnostic strategies, interpret clinical-trial results for vaccines and therapeutics, model viral transmission, and understand virus-host interactions. H…
View article: Why Daily SARS-CoV-2 Nasal Rapid Antigen Testing Poorly Detects Infected and Infectious Individuals
Why Daily SARS-CoV-2 Nasal Rapid Antigen Testing Poorly Detects Infected and Infectious Individuals Open
Background In a recent household-transmission study of SARS-CoV-2, we found extreme differences in SARS-CoV-2 viral loads among paired saliva, anterior-nares swab (ANS) and oropharyngeal swab specimens collected from the same timepoint. We…
View article: Quantitative whole-tissue 3D imaging reveals bacteria in close association with mouse jejunum mucosa
Quantitative whole-tissue 3D imaging reveals bacteria in close association with mouse jejunum mucosa Open
Background The small intestine (SI) is the primary site of nutrient absorption, so its large surface area lacks the thick protective mucus that is characteristic of the large intestine. Because the SI epithelium is relatively exposed, any …
View article: Microbial Sources of Exocellular DNA in the Ocean
Microbial Sources of Exocellular DNA in the Ocean Open
With advances in metagenomic sequencing, the microbial composition of diverse environmental systems has been investigated, providing new perspectives on potential ecological dynamics and dimensions for experimental investigations. Here, we…
View article: Morning SARS-CoV-2 testing yields better detection of infection due to higher viral loads in saliva and nasal swabs upon waking
Morning SARS-CoV-2 testing yields better detection of infection due to higher viral loads in saliva and nasal swabs upon waking Open
Background The analytical sensitivities of SARS-CoV-2 diagnostic tests span 6 orders of magnitude. Optimizing sample-collection methods to achieve the most reliable detection for a given sensitivity would increase the effectiveness of test…
View article: Evidence of Genomic Diversification in a Natural Symbiotic Population Within Its Host
Evidence of Genomic Diversification in a Natural Symbiotic Population Within Its Host Open
Planktonic cells of the luminous marine bacterium Vibrio fischeri establish themselves in the light-emitting organ of each generation of newly hatched Euprymna scolopes bobtail squid. A symbiont population is maintained within the 6 separa…
View article: Quantitative SARS-CoV-2 Viral-Load Curves in Paired Saliva Samples and Nasal Swabs Inform Appropriate Respiratory Sampling Site and Analytical Test Sensitivity Required for Earliest Viral Detection
Quantitative SARS-CoV-2 Viral-Load Curves in Paired Saliva Samples and Nasal Swabs Inform Appropriate Respiratory Sampling Site and Analytical Test Sensitivity Required for Earliest Viral Detection Open
Early detection of SARS-CoV-2 infection is critical to reduce asymptomatic and presymptomatic transmission, curb the spread of variants, and maximize treatment efficacy. Low-analytical-sensitivity nasal-swab testing is commonly used for su…
View article: Microbial sources of exocellular DNA in the ocean
Microbial sources of exocellular DNA in the ocean Open
Dissolved DNA (D-DNA) is operationally defined as DNA that passes through a 0.1 micrometer filter. It is composed of vesicles, viruses, and exocellular free DNA (F-DNA) and is ubiquitous in all aquatic systems, although the sources, sinks,…
View article: Quantitative SARS-CoV-2 viral-load curves in paired saliva and nasal swabs inform appropriate respiratory sampling site and analytical test sensitivity required for earliest viral detection
Quantitative SARS-CoV-2 viral-load curves in paired saliva and nasal swabs inform appropriate respiratory sampling site and analytical test sensitivity required for earliest viral detection Open
Early detection of SARS-CoV-2 infection is critical to reduce asymptomatic and pre-symptomatic transmission, curb the spread of variants by travelers, and maximize treatment efficacy. Low-sensitivity nasal-swab testing (antigen and some nu…
View article: Additional file 3 of Quantitative sequencing clarifies the role of disruptor taxa, oral microbiota, and strict anaerobes in the human small-intestine microbiome
Additional file 3 of Quantitative sequencing clarifies the role of disruptor taxa, oral microbiota, and strict anaerobes in the human small-intestine microbiome Open
Additional file 2.
View article: SARS-CoV-2 Viral Load in Saliva Rises Gradually and to Moderate Levels in Some Humans
SARS-CoV-2 Viral Load in Saliva Rises Gradually and to Moderate Levels in Some Humans Open
Transmission of SARS-CoV-2 in community settings often occurs before symptom onset, therefore testing strategies that can reliably detect people in the early phase of infection are urgently needed. Early detection of SARS-CoV-2 infection i…
View article: Diagnosis and management of the drug hypersensitivity reactions in Coronavirus disease 19
Diagnosis and management of the drug hypersensitivity reactions in Coronavirus disease 19 Open
Coronavirus disease 2019 (COVID-19), a respiratory tract infection caused by a novel human coronavirus, the severe acute respiratory syndrome coronavirus 2, leads to a wide spectrum of clinical manifestations ranging from asymptomatic case…
View article: Double-stranded DNA virioplankton dynamics and reproductive strategies in the oligotrophic open ocean water column
Double-stranded DNA virioplankton dynamics and reproductive strategies in the oligotrophic open ocean water column Open
Microbial communities are critical to ecosystem dynamics and biogeochemical cycling in the open oceans. Viruses are essential elements of these communities, influencing the productivity, diversity, and evolution of cellular hosts. To furth…
View article: Diel Oscillation of Microbial Gene Transcripts Declines With Depth in Oligotrophic Ocean Waters
Diel Oscillation of Microbial Gene Transcripts Declines With Depth in Oligotrophic Ocean Waters Open
Diel oscillations in primary and secondary production, growth, metabolic activity, and gene expression commonly occur in marine microbial communities in ocean surface waters. Diel periodicity of gene transcription has been demonstrated in …
View article: Autonomous Tracking and Sampling of the Deep Chlorophyll Maximum Layer in an Open-Ocean Eddy by a Long-Range Autonomous Underwater Vehicle
Autonomous Tracking and Sampling of the Deep Chlorophyll Maximum Layer in an Open-Ocean Eddy by a Long-Range Autonomous Underwater Vehicle Open
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Zhang, Y., Kieft, B., Hobson, B. W., Ryan, J. P., Barone, B., Preston, C. M., Roman, B.…
View article: Diel cycling and long-term persistence of viruses in the ocean’s euphotic zone
Diel cycling and long-term persistence of viruses in the ocean’s euphotic zone Open
Significance Marine microbial communities exert a large influence on ocean ecosystem processes, and viruses in these communities play key roles in controlling microbial abundances, nutrient cycling, and productivity. We show here that domi…