Anna Lisa Giuliani
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View article: Neuroinflammation in Alzheimer's disease: the role of the P2X7 receptor (P2X7R)
Neuroinflammation in Alzheimer's disease: the role of the P2X7 receptor (P2X7R) Open
Background Growing evidence underscores the central role of neuroinflammation in Alzheimer's Disease (AD). In inflamed brain accumulation of pro‐inflammatory molecules promote the activation of resident immune and accessory cells, i.e. mic…
View article: The purinergic signaling in Alzheimer's disease: possible use of a shed form of the P2X7 receptor (sP2X7R) as a biomarker of early dementia
The purinergic signaling in Alzheimer's disease: possible use of a shed form of the P2X7 receptor (sP2X7R) as a biomarker of early dementia Open
Background Neuroinflammation, one of the recognised factors in AD, is likely sustained by the presence of high amounts of extracellular ATP (eATP) in the inflammed brain. One main goal of our study was the measurement of eATP levels in the…
View article: Insight Into the Cellular Activities of Tranexamic Acid as an Option for Melasma Treatment
Insight Into the Cellular Activities of Tranexamic Acid as an Option for Melasma Treatment Open
Melasma and other hyperpigmentation disorders represent a significant challenge in dermatology. Tranexamic acid (TXA), traditionally known for its antifibrinolytic activity via inhibition of plasminogen activation, has recently emerged as …
View article: Editorial: Purinergic signaling in metabolic diseases and inflammation pharmacology
Editorial: Purinergic signaling in metabolic diseases and inflammation pharmacology Open
Numerous diseases, including obesity, diabetes, cardiovascular disorders, dementia-associated cognitive decline and sarcopenia are characterized by underlying inflammatory processes that contribute to disease progression. Purinergic signal…
View article: Overview of the role of purinergic signaling and insights into its role in cancer therapy
Overview of the role of purinergic signaling and insights into its role in cancer therapy Open
Innovation of cancer therapy has received a dramatic acceleration over the last fifteen years thanks to the introduction of the novel immune checkpoint inhibitors (ICI). On the other hand, the conspicuous scientific knowledge accumulated i…
View article: #2498 PKD2 recurrent haplotype in ADPKD patients
#2498 PKD2 recurrent haplotype in ADPKD patients Open
Background and Aims Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a systemic renal ciliopathy characterized by progressive renal cyst expansion and extrarenal manifestations following loss-of-function mutations. ADPKD is primaril…
View article: The P2X7 Receptor is a Master Regulator of Microparticle and Mitochondria Exchange in Mouse Microglia
The P2X7 Receptor is a Master Regulator of Microparticle and Mitochondria Exchange in Mouse Microglia Open
Microparticles (MPs) are secreted by all cells, where they play a key role in intercellular communication, differentiation, inflammation, and cell energy transfer. P2X7 receptor (P2X7R) activation by extracellular ATP (eATP) causes a large…
View article: The Coming of Age of the P2X7 Receptor in Diagnostic Medicine
The Coming of Age of the P2X7 Receptor in Diagnostic Medicine Open
The discovery of the P2X7 receptor (P2X7R, originally named P2Z) in immune cells, its cloning, and the identification of its role in a multiplicity of immune-mediated diseases raised great hopes for the development of novel and more potent…
View article: The shed P2X7 receptor is an index of adverse clinical outcome in COVID-19 patients
The shed P2X7 receptor is an index of adverse clinical outcome in COVID-19 patients Open
Introduction The pathophysiology of the Corona Virus Disease 2019 (COVID-19) is incompletely known. A robust inflammatory response caused by viral replication is a main cause of the acute lung and multiorgan injury observed in critical pat…
View article: P2X7-dependent exchange of extracellular microparticles and mitochondria by mouse microglia
P2X7-dependent exchange of extracellular microparticles and mitochondria by mouse microglia Open
Microparticles (MPs) are ubiquitously secreted by all cells and play a fundamental role in numerous biological processes such as cell-to-cell communication, cell differentiation, inflammation, and cell energy transfer. Ligation of the P2X7…
View article: Data from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Data from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
The P2X7 receptor is an ATP-gated ion channel known for its cytotoxic activity. However, recent evidence suggests a role for P2X7 in cell proliferation. Here, we found that P2X7 exhibits significant growth-promoting effects in vivo. Human …
View article: Figure S2 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
Figure S2 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
Figure S2. Core necrosis in B16 melanomas. Representative images of central necrosis of B16 melanoma masses in P2X7R-KO (A and B) or wt (C and D) mice. Specimens were fixed and stained with haematoxylin-eosin as described in Materials and …
View article: Figure S3 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
Figure S3 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
Figure S3. Effect of P2X7R deletion on VEGF release. A, in vitro VEGF release from wt or P2X7R-silenced B16 cells. B, intra-tumor VEGF content of B16-wt or P2X7R-silenced tumors grown in wt or P2X7R-KO mice. VEGF was measured by ELISA. A, …
View article: Data from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
Data from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
The ATP receptor P2X7 (P2X7R or P2RX7) has a key role in inflammation and immunity, but its possible roles in cancer are not firmly established. In the present study, we investigated the effect of host genetic deletion of P2X7R in the mous…
View article: SMovie S4 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
SMovie S4 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
SMovie S4. Migration of wt N13 microglial cells from both sides of a wounded monolayer after scratching with a plastic pipette tip.
View article: SMovie S4 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
SMovie S4 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
SMovie S4. Migration of wt N13 microglial cells from both sides of a wounded monolayer after scratching with a plastic pipette tip.
View article: Supplementary Figure 4 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 4 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 72KB, Fig. S4. Effect of P2X7 silencing on ACN neuroblastoma growth. Panel A, P2X7 expression in ACN neuroblastoma cells transfected with P2X7-specific or scrambled shRNA. Cell protein was extracted, run on SDS PAGE, blotted and …
View article: Figure S1 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
Figure S1 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
Figure S1. Characterization of P2X7R shRNA B16 cells. A, densitometry of P2X7R protein expression. B, representative Western blot. C, Fura-2 measurement of Benzoyl ATP (BzATP)-triggered intracellular Ca2+ increase. D, Averages intracellula…
View article: Supplementary Figure 4 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 4 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 72KB, Fig. S4. Effect of P2X7 silencing on ACN neuroblastoma growth. Panel A, P2X7 expression in ACN neuroblastoma cells transfected with P2X7-specific or scrambled shRNA. Cell protein was extracted, run on SDS PAGE, blotted and …
View article: Supplementary Figure 3 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 3 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 56KB, Fig. S3. Effect of P2X7 inhibition on B16 melanoma growth. Panel A, comparison of basal P2X7 expression in HEK293-mock, CT26wt and B16 tumor cell lines. Cell protein was extracted, run on SDS PAGE, blotted and stained with …
View article: Figure S3 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
Figure S3 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
Figure S3. Effect of P2X7R deletion on VEGF release. A, in vitro VEGF release from wt or P2X7R-silenced B16 cells. B, intra-tumor VEGF content of B16-wt or P2X7R-silenced tumors grown in wt or P2X7R-KO mice. VEGF was measured by ELISA. A, …
View article: Supplementary Figure 2 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 2 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 88KB, Fig. S2. Effect of P2X7 expression on the growth of CT26 colon carcinoma. Panel A, detection of mP2X7 by semiquantitative PCR and Western blot in CT26-wt cells and in three stably transfected clones (CT26-mP2X7c1, c2, and c…
View article: Data from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
Data from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
The ATP receptor P2X7 (P2X7R or P2RX7) has a key role in inflammation and immunity, but its possible roles in cancer are not firmly established. In the present study, we investigated the effect of host genetic deletion of P2X7R in the mous…
View article: Figure S1 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
Figure S1 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
Figure S1. Characterization of P2X7R shRNA B16 cells. A, densitometry of P2X7R protein expression. B, representative Western blot. C, Fura-2 measurement of Benzoyl ATP (BzATP)-triggered intracellular Ca2+ increase. D, Averages intracellula…
View article: Supplementary Figure 1 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 1 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 156KB, Fig. S1. Immuno staining with anti-P2X7 Ab of HEK293-mock (panel A) or HEK293-hP2X7 (panel B). Panels C-F, distinct morphological patterns of HEK293-mock and HEK293-hP2X7 cells in vivo (C and D) and in vitro (E and F). Pan…
View article: Supplementary Figure 2 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 2 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 88KB, Fig. S2. Effect of P2X7 expression on the growth of CT26 colon carcinoma. Panel A, detection of mP2X7 by semiquantitative PCR and Western blot in CT26-wt cells and in three stably transfected clones (CT26-mP2X7c1, c2, and c…
View article: Supplementary Figure 1 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 1 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 156KB, Fig. S1. Immuno staining with anti-P2X7 Ab of HEK293-mock (panel A) or HEK293-hP2X7 (panel B). Panels C-F, distinct morphological patterns of HEK293-mock and HEK293-hP2X7 cells in vivo (C and D) and in vitro (E and F). Pan…
View article: Supplementary Figure 3 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth
Supplementary Figure 3 from Expression of P2X7 Receptor Increases <i>In Vivo</i> Tumor Growth Open
PDF file, 56KB, Fig. S3. Effect of P2X7 inhibition on B16 melanoma growth. Panel A, comparison of basal P2X7 expression in HEK293-mock, CT26wt and B16 tumor cell lines. Cell protein was extracted, run on SDS PAGE, blotted and stained with …
View article: SMovie S5 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7
SMovie S5 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 Open
SMovie S5. N13R microglial cells are unable to migrate in response to wounding.