Anna Placci
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View article: Hollow fiber flow field-flow fractionation enables high-quality Extracellular Vesicle isolation from minimal plasma samples in polycythemia vera liquid biopsy
Hollow fiber flow field-flow fractionation enables high-quality Extracellular Vesicle isolation from minimal plasma samples in polycythemia vera liquid biopsy Open
HF5 integrates EV isolation and characterization, enhancing efficiency, and preserving sample integrity and functionality. Its minimal sample requirement and reproducibility make it particularly suitable for clinical and translational rese…
View article: A direct, real-time, size-resolved analytical strategy to follow drug loading and release from biocompatible gold nanoparticles
A direct, real-time, size-resolved analytical strategy to follow drug loading and release from biocompatible gold nanoparticles Open
The tailored strategy employed in our investigation provided detailed, real-time, in situ analysis, making it a powerful tool for designing and optimizing drug delivery systems, providing insight into both loading and release mechanisms, a…
View article: Multitechnique characterization of eco-corona formation on airborne nanoplastics
Multitechnique characterization of eco-corona formation on airborne nanoplastics Open
A profilomic approach used AF4, UV/Vis and Raman to investigate pollen–NanoPET hybrid bio-nano structures and eco-coronas. This green, multidimensional analytical workflow gave insight on nanoplastics behaviour in complex biological matric…
View article: Emerging Microfluidic Tools for Simultaneous Exosomes and Cargo Biosensing in Liquid Biopsy: New Integrated Miniaturized FFF-Assisted Approach for Colon Cancer Diagnosis
Emerging Microfluidic Tools for Simultaneous Exosomes and Cargo Biosensing in Liquid Biopsy: New Integrated Miniaturized FFF-Assisted Approach for Colon Cancer Diagnosis Open
The early-stage diagnosis of cancer is a crucial clinical need. The inadequacies of surgery tissue biopsy have prompted a transition to a less invasive profiling of molecular biomarkers from biofluids, known as liquid biopsy. Exosomes are …
View article: Field-Flow Fractionation in Molecular Biology and Biotechnology
Field-Flow Fractionation in Molecular Biology and Biotechnology Open
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension. These techniques cover an extremely wide dynamic range and are able to sep…
View article: The achievement of boar sperm<i>in vitro</i>capacitation is related to an increase of disrupted disulphide bonds and intracellular reactive oxygen species levels
The achievement of boar sperm<i>in vitro</i>capacitation is related to an increase of disrupted disulphide bonds and intracellular reactive oxygen species levels Open
Summary The aim of this work was to determine the relationship of intracellular reactive oxygen species (ROS) and the disulphide bonds established between sperm proteins with the achievement of capacitation in boar spermatozoa. With this p…
View article: Melatonin affects the motility and adhesiveness of in vitro capacitated boar spermatozoa via a mechanism that does not depend on intracellular <scp>ROS</scp> levels
Melatonin affects the motility and adhesiveness of in vitro capacitated boar spermatozoa via a mechanism that does not depend on intracellular <span>ROS</span> levels Open
Summary This work sought to address the effects of melatonin during in vitro capacitation ( IVC ) and progesterone‐induced acrosome exocytosis ( IVAE ) in boar spermatozoa. With this purpose, two different experiments were set. In the firs…