Anna Ściuk
YOU?
Author Swipe
View article: Encapsulated Mn-Saturated Lactoferrin as a Safe Source of Manganese Ions for Restoring Probiotic Lactobacillus plantarum
Encapsulated Mn-Saturated Lactoferrin as a Safe Source of Manganese Ions for Restoring Probiotic Lactobacillus plantarum Open
The growth of Lactobacillus plantarum, a member of the Lactobacillus genus, which plays a crucial role in the bacterial microbiome of the gut, is significantly influenced by manganese ions. They can be safely delivered to the intestines by…
View article: Substrate Affinity Is Not Crucial for Therapeutic L-Asparaginases: Antileukemic Activity of Novel Bacterial Enzymes
Substrate Affinity Is Not Crucial for Therapeutic L-Asparaginases: Antileukemic Activity of Novel Bacterial Enzymes Open
L-asparaginases are used in the treatment of acute lymphoblastic leukemia. The aim of this work was to compare the antiproliferative potential and proapoptotic properties of novel L-asparaginases from different structural classes, viz. EcA…
View article: Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis
Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis Open
This report describes a comprehensive approach to local random mutagenesis of the E. coli Ntn-amidohydrolase EcAIII, and supplements the results published earlier for the randomization series RDM1. Here, random mutagenesis was applied in t…
View article: The effects of nature‐inspired amino acid substitutions on structural and biochemical properties of the <scp><i>E. coli</i> L</scp>‐asparaginase <scp>EcAIII</scp>
The effects of nature‐inspired amino acid substitutions on structural and biochemical properties of the <span><i>E. coli</i> L</span>‐asparaginase <span>EcAIII</span> Open
The Escherichia coli enzyme EcAIII catalyzes the hydrolysis of L‐Asn to L‐Asp and ammonia. Using a nature‐inspired mutagenesis approach, we designed and produced five new EcAIII variants (M200I, M200L, M200K, M200T, M200W). The modified pr…