Anne Louise Revenfeld
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View article: Targeted gene editing and near-universal cDNA insertion of CYBA and CYBB as a treatment for chronic granulomatous disease
Targeted gene editing and near-universal cDNA insertion of CYBA and CYBB as a treatment for chronic granulomatous disease Open
View article: Gene editing in hematopoietic stem cells by co-delivery of Cas9/sgRNA ribonucleoprotein and templates for homology-directed repair in ‘all-in-one’ lentivirus-derived nanoparticles
Gene editing in hematopoietic stem cells by co-delivery of Cas9/sgRNA ribonucleoprotein and templates for homology-directed repair in ‘all-in-one’ lentivirus-derived nanoparticles Open
Repair of double-strand DNA breaks generated by site-directed endonucleases, like Cas9, is the hallmark of gene editing based on homology-directed repair (HDR). HDR uses an exogenous DNA template to restore the cleaved DNA sequence and can…
View article: Urine-derived stem cells serve as a robust platform for generating native or engineered extracellular vesicles
Urine-derived stem cells serve as a robust platform for generating native or engineered extracellular vesicles Open
View article: Urine-derived stem cells; A robust platform for generating native or engineered autologous Extracellular Vesicles
Urine-derived stem cells; A robust platform for generating native or engineered autologous Extracellular Vesicles Open
Background Mesenchymal stem cell (MSC) therapy holds great potential as a treatment form, yet efficacy and safety concerns with cell therapy persist. The beneficial effects of MSCs are often attributed to their secretome, including extrace…
View article: Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants
Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants Open
View article: Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation
Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation Open
Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demons…
View article: Presence of <scp>HLA</scp>‐<scp>DR</scp> Molecules and <i><scp>HLA</scp>‐<scp>DRB</scp>1 </i><scp>mRNA</scp> in Circulating <scp>CD</scp>4<sup>+</sup> T Cells
Presence of <span>HLA</span>‐<span>DR</span> Molecules and <i><span>HLA</span>‐<span>DRB</span>1 </i><span>mRNA</span> in Circulating <span>CD</span>4<sup>+</sup> T Cells Open
The human major histocompatibility complex class II isotype HLA ‐ DR is currently used as an activation marker for T cells. However, whether an endogenous protein expression or a molecular acquisition accounts for the presence of HLA ‐ DR …
View article: Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication
Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication Open
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View article: Oxygen-Related Differences in Cellular and Vesicular Phenotypes Observed for Ovarian Cell Cancer Lines
Oxygen-Related Differences in Cellular and Vesicular Phenotypes Observed for Ovarian Cell Cancer Lines Open
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View article: Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication
Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication Open
Appropriate and well-documented in vitro cell-culturing systems are necessary to study the activity and biological function of extracellular vesicles (EVs). The aim of this study was to describe an experimental system, in which dyna…
View article: Phenotyping of leukocytes and leukocyte-derived extracellular vesicles
Phenotyping of leukocytes and leukocyte-derived extracellular vesicles Open
Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great…
View article: Contact-dependent and -independent co-cultures of allogeneic human immune cells yield heterogeneous vesicle phenotype but conserved cellular phenotype
Contact-dependent and -independent co-cultures of allogeneic human immune cells yield heterogeneous vesicle phenotype but conserved cellular phenotype Open
View article: Oxygen-Related Differences in Cellular and Vesicular Phenotypes Observed for Ovarian Cell Cancer Lines
Oxygen-Related Differences in Cellular and Vesicular Phenotypes Observed for Ovarian Cell Cancer Lines Open
Extracellular vesicles (EVs) are one of several tools that cells use to communicate with each other. This communication is facilitated by a number of surface-associated proteins and the cargo of the vesicles. For several cancer types, the …
View article: Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles
Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles Open
Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great…
View article: Extensive analysis of four ovarian cancer cell lines and their production of extracellular vesicles during normoxic and hypoxic conditions
Extensive analysis of four ovarian cancer cell lines and their production of extracellular vesicles during normoxic and hypoxic conditions Open
View article: Antibody-Based Assays for Phenotyping of Extracellular Vesicles
Antibody-Based Assays for Phenotyping of Extracellular Vesicles Open
Extracellular vesicles (EVs) are a heterogeneous population of membrane-enclosed vesicles. EVs are recognized as important players in cell-to-cell communication and are described to be involved in numerous biological and pathological proce…