Annett Neuner
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View article: The interplay between Wnt and mTOR signaling modulates ciliogenesis in human retinal epithelial cells
The interplay between Wnt and mTOR signaling modulates ciliogenesis in human retinal epithelial cells Open
The primary cilium is a microtubule-based organelle essential for various cellular functions, particularly signal transduction. While the role of cilia in regulating signaling pathways has been extensively studied, the impact of signaling …
View article: Conserved function of the HAUS6 calponin homology domain in anchoring augmin for microtubule branching
Conserved function of the HAUS6 calponin homology domain in anchoring augmin for microtubule branching Open
View article: Multifunctional Roles of Brr6 and Brl1 in Nuclear Envelope Fusion During Nuclear Pore Complex Biogenesis
Multifunctional Roles of Brr6 and Brl1 in Nuclear Envelope Fusion During Nuclear Pore Complex Biogenesis Open
Summary Brl1 and Brr6 are essential, paralogous integral membrane proteins of the yeast nuclear envelope (NE) that transiently associate with nuclear pore complexes (NPCs) during their assembly to promote fusion of the inner (INM) and oute…
View article: Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC
Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC Open
The γ-tubulin ring complex (γ-TuRC) acts as a structural template for microtubule formation at centrosomes, associating with two main compartments: the pericentriolar material and the centriole lumen. In the pericentriolar material, the γ-…
View article: Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors
Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors Open
The γ-tubulin ring complex (γ-TuRC) is a structural template for controlled nucleation of microtubules from α/β-tubulin heterodimers. At the cytoplasmic side of the yeast spindle pole body, the CM1-containing receptor protein Spc72 promote…
View article: An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity
An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity Open
View article: γ-TuRC asymmetry induces local protofilament mismatch at the RanGTP-stimulated microtubule minus end
γ-TuRC asymmetry induces local protofilament mismatch at the RanGTP-stimulated microtubule minus end Open
View article: The central scaffold protein CEP350 coordinates centriole length, stability, and maturation
The central scaffold protein CEP350 coordinates centriole length, stability, and maturation Open
The centriole is the microtubule-based backbone that ensures integrity, function, and cell cycle–dependent duplication of centrosomes. Mostly unclear mechanisms control structural integrity of centrioles. Here, we show that the centrosome …
View article: The augmin complex architecture reveals structural insights into microtubule branching
The augmin complex architecture reveals structural insights into microtubule branching Open
In mitosis, the augmin complex binds to spindle microtubules to recruit the γ-tubulin ring complex (γ-TuRC), the principal microtubule nucleator, for the formation of branched microtubules. Our understanding of augmin-mediated microtubule …
View article: Modular assembly of the principal microtubule nucleator γ-TuRC
Modular assembly of the principal microtubule nucleator γ-TuRC Open
View article: Balancing the length of the distal tip by septins is key for stability and signalling function of primary cilia
Balancing the length of the distal tip by septins is key for stability and signalling function of primary cilia Open
View article: Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation
Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation Open
Nuclear pore complexes (NPCs) are channels within the nuclear envelope that mediate nucleocytoplasmic transport. NPCs form within the closed nuclear envelope during interphase or assemble concomitantly with nuclear envelope reformation in …
View article: A short perinuclear amphipathic α-helix in Apq12 promotes nuclear pore complex biogenesis
A short perinuclear amphipathic α-helix in Apq12 promotes nuclear pore complex biogenesis Open
The integral membrane protein Apq12 is an important nuclear envelope (NE)/endoplasmic reticulum (ER) modulator that cooperates with the nuclear pore complex (NPC) biogenesis factors Brl1 and Brr6. How Apq12 executes these functions is unkn…
View article: A short perinuclear amphipathic α-helix in Apq12 promotes nuclear pore complex biogenesis
A short perinuclear amphipathic α-helix in Apq12 promotes nuclear pore complex biogenesis Open
Summary The integral membrane protein Apq12 is an important nuclear envelope (NE)/ER modulator that cooperates with the nuclear pore complex (NPC) biogenesis factors Brl1 and Brr6. How Apq12 executes these functions is unknown. Here we ide…
View article: Balancing the length of distal tip is key for stability and signalling function of primary cilia
Balancing the length of distal tip is key for stability and signalling function of primary cilia Open
Primary cilia are antenna-like organelles required for signalling transduction. How cilia structure is mechanistically maintained at steady-state to promote signalling is largely unknown. Here, we define that mammalian primary cilia are fo…
View article: The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body
The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body Open
The spindle pole body (SPB) provides microtubule-organizing functions in yeast and duplicates exactly once per cell cycle. The first step in SPB duplication is the half-bridge to bridge conversion via the antiparallel dimerization of the c…
View article: Reconstitution of the recombinant human γ-tubulin ring complex
Reconstitution of the recombinant human γ-tubulin ring complex Open
Cryo-electron microscopy recently resolved the structure of the vertebrate γ-tubulin ring complex (γ-TuRC) purified from Xenopus laevis egg extract and human cells to near-atomic resolution. These studies clarified the arrangement and stoi…
View article: The cryo-EM structure of a γ-TuSC elucidates architecture and regulation of minimal microtubule nucleation systems
The cryo-EM structure of a γ-TuSC elucidates architecture and regulation of minimal microtubule nucleation systems Open
The nucleation of microtubules from αβ-tubulin subunits is mediated by γ-tubulin complexes, which vary in composition across organisms. Aiming to understand how de novo microtubule formation is achieved and regulated by a minimal microtubu…
View article: Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis
Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis Open
Distal appendages (DAs) of the mother centriole are essential for the initial steps of ciliogenesis in G1/G0 phase of the cell cycle. DAs are released from centrosomes in mitosis by an undefined mechanism. Here, we show that specific DAs l…
View article: CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion
CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion Open
View article: The human phosphatase <scp>CDC</scp> 14A modulates primary cilium length by regulating centrosomal actin nucleation
The human phosphatase <span>CDC</span> 14A modulates primary cilium length by regulating centrosomal actin nucleation Open
View article: The microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation
The microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation Open
Stu2/XMAP215/ZYG-9/Dis1/Alp14/Msps/ch-TOG family members in association with with γ-tubulin complexes nucleate microtubules, but we know little about the interplay of these nucleation factors. Here, we show that the budding yeast Stu2 in c…
View article: Author response: The microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation
Author response: The microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Stu2/XMAP215/ZYG-9/Dis1/Alp14/Msps/ch-TOG …
View article: LRRC45 contributes to early steps of axoneme extension
LRRC45 contributes to early steps of axoneme extension Open
Cilia perform essential signalling functions during development and tissue homeostasis. A key event in ciliogenesis occurs when the distal appendages of the mother centriole form a platform that docks ciliary vesicles and removes CP110-Cep…
View article: Brr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis
Brr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis Open
The paralogous Brr6 and Brl1 are conserved integral membrane proteins of the nuclear envelope (NE) with an unclear role in nuclear pore complex (NPC) biogenesis. Here, we analyzed double-degron mutants of Brr6/Brl1 to understand this funct…
View article: Analysis of LRRC45 indicates cooperative functions of distal appendages at early steps of ciliogenesis
Analysis of LRRC45 indicates cooperative functions of distal appendages at early steps of ciliogenesis Open
Cilia perform essential signalling functions during development and tissue homeostasis. Ciliary malfunction causes a variety of diseases, named ciliopathies. The key role that the mother centriole plays in cilia formation can be attributed…
View article: Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha
Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha Open
Cytoplasmic microtubules (cMT) control mitotic spindle positioning in many organisms, and are therefore pivotal for successful cell division. Despite its importance, the temporal control of cMT formation remains poorly understood. Here we …
View article: Author response: Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha
Author response: Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Cytoplasmic microtubules (cMT…
View article: Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes
Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes Open
The spindle pole body (SPB) of budding yeast duplicates once per cell cycle. In G1, the satellite, an SPB precursor, assembles next to the mother SPB (mSPB) on the cytoplasmic side of the nuclear envelope (NE). How the growing satellite su…
View article: MOZART1 and γ-tubulin complex receptors are both required to turn γ-TuSC into an active microtubule nucleation template
MOZART1 and γ-tubulin complex receptors are both required to turn γ-TuSC into an active microtubule nucleation template Open
MOZART1/Mzt1 is required for the localization of γ-tubulin complexes to microtubule (MT)–organizing centers from yeast to human cells. Nevertheless, the molecular function of MOZART1/Mzt1 is largely unknown. Taking advantage of the minimal…