Anne Diehl
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View article: Changes in Secondary Structure Upon Pr to Pfr Transition in Cyanobacterial Phytochrome Cph1 Detected by DNP NMR
Changes in Secondary Structure Upon Pr to Pfr Transition in Cyanobacterial Phytochrome Cph1 Detected by DNP NMR Open
Phytochromes perceive subtle changes in the light environment and convert them into biological signals by photoconversion between the red‐light absorbing (Pr) and the far‐red‐absorbing (Pfr) states. In the primitive bacteriophytochromes th…
View article: Soft X-ray tomography reveals variations in<i>B.subtilis</i>biofilm structure upon<i>tasA</i>deletion
Soft X-ray tomography reveals variations in<i>B.subtilis</i>biofilm structure upon<i>tasA</i>deletion Open
Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture r…
View article: Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects Open
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa whereas magic-angle-spinning (MAS) solid-state NMR traditionally targets very large, oligomeric proteins and complexes exceeding 500 kDa in mass, inclu…
View article: Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects Open
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa whereas magic-angle-spinning (MAS) solid-state NMR traditionally targets very large, oligomeric proteins and complexes exceeding 500 kDa in mass, inclu…
View article: TapA acts as specific chaperone in TasA filament formation by strand complementation
TapA acts as specific chaperone in TasA filament formation by strand complementation Open
Studying mechanisms of bacterial biofilm generation is of vital importance to understanding bacterial cell–cell communication, multicellular cohabitation principles, and the higher resilience of microorganisms in a biofilm against antibiot…
View article: A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry
A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry Open
Cross‐linking mass spectrometry (XL‐MS) is an attractive method for the proteome‐wide characterization of protein structures and interactions. Currently, the depth of in vivo XL‐MS studies is lagging behind the established applications to …
View article: A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry
A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry Open
Cross‐linking mass spectrometry (XL‐MS) is an attractive method for the proteome‐wide characterization of protein structures and interactions. Currently, the depth of in vivo XL‐MS studies is lagging behind the established applications to …
View article: Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling Open
Dishevelled (Dvl) proteins are important regulators of the Wnt signalling pathway, interacting through their PDZ domains with the Wnt receptor Frizzled. Blocking the Dvl PDZ–Frizzled interaction represents a potential approach for cancer t…
View article: Supplementary material to "Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling"
Supplementary material to "Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling" Open
Tables of contents 1. Structure-based alignment of the amino acid sequences of Dvl-1,2,3 PDZ ; PSD95-PDZ-1,2,3 ; Af-6 and Syn PDZ domains.(S.2) 2. 1H-15N HSQC spectra of Dvl-3 PDZ domain alone and in the presence of varying concentrations …
View article: Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling Open
Dishevelled (Dvl) proteins are important regulators of the Wnt signalling pathway, interacting through their PDZ domains with the Wnt receptor Frizzled. Blocking the Dvl PDZ/Frizzled interaction represents a potential approach for cancer t…
View article: Two step PCR method to exchange the resistance cassette of a vector
Two step PCR method to exchange the resistance cassette of a vector Open
For co-transformation of two plasmids, both have to possess different antibiotic selection markers. If that is not the case, normally the gene of interest (GOI) is subcloned into another vector. Here we introduce a fast and easy method to …
View article: pH‐Dependent Protonation of Surface Carboxylate Groups in PsbO Enables Local Buffering and Triggers Structural Changes
pH‐Dependent Protonation of Surface Carboxylate Groups in PsbO Enables Local Buffering and Triggers Structural Changes Open
Photosystem II (PSII) catalyzes the splitting of water, releasing protons and dioxygen. Its highly conserved subunit PsbO extends from the oxygen‐evolving center (OEC) into the thylakoid lumen and stabilizes the catalytic Mn 4 CaO 5 cluste…
View article: A molecular mechanism for salt stress-induced microtubule array formation in Arabidopsis
A molecular mechanism for salt stress-induced microtubule array formation in Arabidopsis Open
Microtubules are filamentous structures necessary for cell division, motility and morphology, with dynamics critically regulated by microtubule-associated proteins (MAPs). We outline the molecular mechanism by which the MAP, COMPANION OF C…
View article: The protofilament architecture of a de novo designed coiled coil-based amyloidogenic peptide
The protofilament architecture of a de novo designed coiled coil-based amyloidogenic peptide Open
Amyloid fibrils are polymers formed by proteins under specific conditions and in many cases they are related to pathogenesis, such as Parkinson's and Alzheimer's diseases. Their hallmark is the presence of a β-sheet structure. High resolut…
View article: Structural changes of TasA in biofilm formation of <i>Bacillus subtilis</i>
Structural changes of TasA in biofilm formation of <i>Bacillus subtilis</i> Open
Significance Understanding the formation and structure of protective bacterial biofilms will help to design and identify antimicrobial strategies. Our experiments with the secreted major biofilm protein TasA characterize on a molecular lev…
View article: Structural insight into protein-aided bacterial biofilm formation
Structural insight into protein-aided bacterial biofilm formation Open
Bacterial cells can form surface-associated sessile communities often described as biofilms.The development and establishment of this structured habitat is genetically programmed and consists commonly of exopoly¬saccharides (EPS), extracel…