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View article: From sequence to scaffold: Computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks
From sequence to scaffold: Computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks Open
Self-assembling protein nanoparticles are being increasingly utilized in the design of next-generation vaccines due to their ability to induce antibody responses of superior magnitude, breadth, and durability. Computational protein design …
View article: From sequence to scaffold: computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks
From sequence to scaffold: computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks Open
Self-assembling protein nanoparticles are being increasingly utilized in the design of next-generation vaccines due to their ability to induce antibody responses of superior magnitude, breadth, and durability. Computational protein design …
View article: Stabilization of H5 highly pathogenic avian influenza hemagglutinin improves vaccine-elicited neutralizing antibody responses
Stabilization of H5 highly pathogenic avian influenza hemagglutinin improves vaccine-elicited neutralizing antibody responses Open
Transmission of highly pathogenic avian influenza from H5 clade 2.3.4.4b has expanded in recent years to infect large populations of birds and mammals, heightening the risk of a human pandemic. Influenza viruses adapted to transmission in …
View article: Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV
Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV Open
View article: Hierarchical design of pseudosymmetric protein nanocages
Hierarchical design of pseudosymmetric protein nanocages Open
View article: Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV
Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV Open
Middle East respiratory syndrome coronavirus (MERS-CoV) is a betacoronavirus that causes severe respiratory illness in humans. There are no licensed vaccines against MERS-CoV and only a few candidates in phase I clinical trials. Here, we d…
View article: Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance
Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance Open
H5 influenza is considered a potential pandemic threat. Recently, H5 viruses belonging to clade 2.3.4.4b have caused large outbreaks in avian and multiple nonhuman mammalian species. Previous studies have identified molecular phenotypes of…
View article: Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance
Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance Open
H5 influenza is a potential pandemic threat. Previous studies have identified molecular phenotypes of the viral hemagglutinin (HA) protein that contribute to pandemic risk, including cell entry, receptor preference, HA stability, and reduc…
View article: Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV
Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV Open
Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic betacoronavirus that causes severe and often lethal respiratory illness in humans. The MERS-CoV spike (S) protein is the viral fusogen and the target of neutralizing ant…
View article: Antigen spacing on protein nanoparticles influences antibody responses to vaccination
Antigen spacing on protein nanoparticles influences antibody responses to vaccination Open
View article: Combinatorial immune refocusing within the influenza hemagglutinin RBD improves cross-neutralizing antibody responses
Combinatorial immune refocusing within the influenza hemagglutinin RBD improves cross-neutralizing antibody responses Open
View article: Combinatorial immune refocusing within the influenza hemagglutinin head elicits cross-neutralizing antibody responses
Combinatorial immune refocusing within the influenza hemagglutinin head elicits cross-neutralizing antibody responses Open
SUMMARY The head domain of influenza hemagglutinin (HA) elicits potently neutralizing yet mostly strain-specific antibodies during infection and vaccination. Here we evaluated a series of immunogens that combined several immunofocusing tec…
View article: Antigen spacing on protein nanoparticles influences antibody responses to vaccination
Antigen spacing on protein nanoparticles influences antibody responses to vaccination Open
SUMMARY Immunogen design approaches aim to control the specificity and quality of antibody responses to enable the creation of next-generation vaccines with improved potency and breadth. However, our understanding of the relationship betwe…
View article: Top-down design of protein nanomaterials with reinforcement learning
Top-down design of protein nanomaterials with reinforcement learning Open
The multisubunit protein assemblies that play critical roles in biology are the result of evolutionary selection for function of the entire assembly, and hence the subunits in structures such as icosahedral viral capsids often fit together…
View article: Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoprotein
Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoprotein Open
View article: Polymerization in the actin ATPase clan regulates hexokinase activity in yeast
Polymerization in the actin ATPase clan regulates hexokinase activity in yeast Open
Polymerization regulates hexokinase activity The yeast hexokinase Glk1 is an actin-fold protein that forms polymers in response to binding its substrates and products. Stoddard et al. now show that Glk1 polymers are structurally distinct f…
View article: Independent evolution of polymerization in the Actin ATPase clan regulates hexokinase activity
Independent evolution of polymerization in the Actin ATPase clan regulates hexokinase activity Open
The actin protein fold is found in cytoskeletal polymers, chaperones, and various metabolic enzymes. Many actin-fold proteins, like the carbohydrate kinases, do not polymerize. We find that Glk1, a Saccharomyces cerevisiae glucokinase, for…
View article: The Glycolytic Enzyme Phosphofructokinase-1 Assembles into Filaments
The Glycolytic Enzyme Phosphofructokinase-1 Assembles into Filaments Open
View article: Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids
Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids Open
View article: Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids
Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids Open
Single particle cryo-electron microscopy (cryoEM) is becoming widely adopted as a tool for structural characterization of biomolecules at near-atomic resolution. Vitrification of the sample to obtain a dense distribution of particles withi…
View article: GPCR-G Protein-β-Arrestin Super-Complex Mediates Sustained G Protein Signaling
GPCR-G Protein-β-Arrestin Super-Complex Mediates Sustained G Protein Signaling Open
View article: ERdj5 Reductase Cooperates with Protein Disulfide Isomerase To Promote Simian Virus 40 Endoplasmic Reticulum Membrane Translocation
ERdj5 Reductase Cooperates with Protein Disulfide Isomerase To Promote Simian Virus 40 Endoplasmic Reticulum Membrane Translocation Open
The nonenveloped polyomavirus (PyV) simian virus 40 (SV40) traffics from the cell surface to the endoplasmic reticulum (ER), where it penetrates the ER membrane to reach the cytosol before mobilizing into the nucleus to cause infection. Pr…