Anthony O. Gramolini
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View article: Systems biology approaches investigating mitochondrial dysfunction in cyanotic heart disease: a systematic review
Systems biology approaches investigating mitochondrial dysfunction in cyanotic heart disease: a systematic review Open
View article: Sarco(endo)plasmic reticulum membrane protein REEP5 regulates mitochondrial dynamics in the mouse heart
Sarco(endo)plasmic reticulum membrane protein REEP5 regulates mitochondrial dynamics in the mouse heart Open
View article: Aggresome Formation in PLNR14Δ Cardiomyopathy: the Role of Fkbp8
Aggresome Formation in PLNR14Δ Cardiomyopathy: the Role of Fkbp8 Open
View article: Proteomic profiling of human heart failure reveals specific and shared molecular responses across etiologies
Proteomic profiling of human heart failure reveals specific and shared molecular responses across etiologies Open
View article: Investigating the role of key sarco(endo)plasmic reticulum shaping proteins on cardiomyocyte structure and development
Investigating the role of key sarco(endo)plasmic reticulum shaping proteins on cardiomyocyte structure and development Open
View article: Characterizing the mechanisms and implications of αT-catenin phospho-regulation at the cardiomyocyte intercalated disc
Characterizing the mechanisms and implications of αT-catenin phospho-regulation at the cardiomyocyte intercalated disc Open
View article: Integral membrane-shaping proteins drive sarco(endo)plasmic reticulum formation and maturation during cardiomyocyte development
Integral membrane-shaping proteins drive sarco(endo)plasmic reticulum formation and maturation during cardiomyocyte development Open
View article: Glyoxal is a superior fixative to formaldehyde in promoting antigenicity and structural integrity in murine cardiac tissues
Glyoxal is a superior fixative to formaldehyde in promoting antigenicity and structural integrity in murine cardiac tissues Open
Our findings underscore the utility of glyoxal as a superior alternative to PFA in cardiac biochemistry research, offering improvements in the preservation of tissue morphology, antigen detection, and protein complex conservation in murine…
View article: Heart failure decompensation with cardiogenic shock exhibits distinct sequential inflammatory profiles
Heart failure decompensation with cardiogenic shock exhibits distinct sequential inflammatory profiles Open
Aims The inflammatory profile of cardiogenic shock (CS) after myocardial infarction affects outcomes; however, little is known about the impact of inflammatory changes in CS caused by acute decompensated heart failure (ADHF‐CS). We measure…
View article: Co-Assembly of Oligo-Urethane Nanoparticles with Defined Lipid Additives to Tailor RNA Delivery into Cells
Co-Assembly of Oligo-Urethane Nanoparticles with Defined Lipid Additives to Tailor RNA Delivery into Cells Open
View article: Dilated cardiomyopathy variant R14del increases phospholamban pentamer stability, blunting dynamic regulation of calcium
Dilated cardiomyopathy variant R14del increases phospholamban pentamer stability, blunting dynamic regulation of calcium Open
View article: SARS-CoV-2 pathogenesis in an angiotensin II–induced heart-on-a-chip disease model and extracellular vesicle screening
SARS-CoV-2 pathogenesis in an angiotensin II–induced heart-on-a-chip disease model and extracellular vesicle screening Open
Adverse cardiac outcomes in COVID-19 patients, particularly those with preexisting cardiac disease, motivate the development of human cell-based organ-on-a-chip models to recapitulate cardiac injury and dysfunction and for screening of car…
View article: Primitive macrophages induce sarcomeric maturation and functional enhancement of developing human cardiac microtissues via efferocytic pathways
Primitive macrophages induce sarcomeric maturation and functional enhancement of developing human cardiac microtissues via efferocytic pathways Open
View article: Noninvasive Quantification of Contractile Dynamics in Cardiac Cells, Spheroids, and Organs-on-a-Chip Using High-Frequency Ultrasound
Noninvasive Quantification of Contractile Dynamics in Cardiac Cells, Spheroids, and Organs-on-a-Chip Using High-Frequency Ultrasound Open
Cell-based models that mimic in vivo heart physiology are poised to make significant advances in cardiac disease modeling and drug discovery. In these systems, cardiomyocyte (CM) contractility is an important functional metric, but current…
View article: Multi‐omic analyses and network biology in cardiovascular disease
Multi‐omic analyses and network biology in cardiovascular disease Open
Heart disease remains a leading cause of death in North America and worldwide. Despite advances in therapies, the chronic nature of cardiovascular diseases ultimately results in frequent hospitalizations and steady rates of mortality. Syst…
View article: Dilated cardiomyopathy variant R14del increases phospholamban pentamer stability, blunting dynamic regulation of cardiac calcium handling
Dilated cardiomyopathy variant R14del increases phospholamban pentamer stability, blunting dynamic regulation of cardiac calcium handling Open
The sarco(endo)plasmic reticulum Ca 2+ ATPase (SERCA) is a membrane transporter that creates and maintains intracellular Ca 2+ stores. In the heart, SERCA is regulated by an inhibitory interaction with the monomeric form of the transmembra…
View article: Proteomics and phosphoproteomics of failing human left ventricle identifies dilated cardiomyopathy-associated phosphorylation of CTNNA3
Proteomics and phosphoproteomics of failing human left ventricle identifies dilated cardiomyopathy-associated phosphorylation of CTNNA3 Open
The prognosis and treatment outcomes of heart failure (HF) patients rely heavily on disease etiology, yet the majority of underlying signaling mechanisms are complex and not fully elucidated. Phosphorylation is a major point of protein reg…
View article: Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure
Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure Open
Inflammation promotes adverse ventricular remodeling, a common antecedent of heart failure. Here, we set out to determine how inflammatory cells affect cardiomyocytes in the remodeling heart. Pathogenic cardiac macrophages induced an IFN r…
View article: An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease
An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease Open
Here, we describe a protocol for purifying functional clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) from Staphylococcus aureus within 24 h and over 90% purity. SaCas9 purification begins wit…
View article: Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure
Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure Open
Heart failure (HF) is a rising global cardiovascular epidemic driven by aging and chronic inflammation. As elderly populations continue to increase, precision treatments for age-related cardiac decline are urgently needed. Here we report t…
View article: Tmem65 is critical for the structure and function of the intercalated discs in mouse hearts
Tmem65 is critical for the structure and function of the intercalated discs in mouse hearts Open
View article: Advanced physiological maturation of iPSC-derived human cardiomyocytes using an algorithm-directed optimization of defined media components
Advanced physiological maturation of iPSC-derived human cardiomyocytes using an algorithm-directed optimization of defined media components Open
Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) hold tremendous promise for in vitro modeling to assess native myocardial function and disease mechanisms as well as testing drug safety and efficacy. However, current iPSC- C…
View article: Vascular tissue engineering from human adipose tissue: fundamental phenotype of its resident microvascular endothelial cells and stromal/stem cells
Vascular tissue engineering from human adipose tissue: fundamental phenotype of its resident microvascular endothelial cells and stromal/stem cells Open
View article: Immunomagnetic Isolation and Enrichment of Microvascular Endothelial Cells from Human Adipose Tissue
Immunomagnetic Isolation and Enrichment of Microvascular Endothelial Cells from Human Adipose Tissue Open
Human adipose tissue-resident microvascular endothelial cells are not only garnering attention for their emergent role in the pathogenesis of obesity-related metabolic disorders, but are also of considerable interest for vascular tissue en…
View article: Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat
Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat Open
Endothelial cells are among the fundamental building blocks for vascular tissue engineering. However, a clinically viable source of endothelium has continued to elude the field. Here, we demonstrate the feasibility of sourcing autologous e…
View article: Deletion of type VIII collagen reduces blood pressure, increases carotid artery functional distensibility and promotes elastin deposition
Deletion of type VIII collagen reduces blood pressure, increases carotid artery functional distensibility and promotes elastin deposition Open
View article: Phosphoproteomic analysis of chondrocytes after short-term exposure to inorganic polyphosphate
Phosphoproteomic analysis of chondrocytes after short-term exposure to inorganic polyphosphate Open
Osteoarthritis is a debilitating disease of the joint that affects over 230 million people worldwide. Currently there are no treatments that slow the progression of this disease. For these reasons, new biological treatment options are curr…
View article: An Efficient and Cost-effective Purification Methodology for SaCas9 Nuclease
An Efficient and Cost-effective Purification Methodology for SaCas9 Nuclease Open
With an ever-increasing demand for laboratory-grade Cas9 proteins by many groups advancing the use of CRISPR technology, a more efficient and scalable process for generating the proteins, coupled with rapid purification methods is in urgen…
View article: Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium
Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium Open
In the current study we examined several proteomic- and RNA-Seq-based datasets of cardiac-enriched, cell-surface and membrane-associated proteins in human fetal and mouse neonatal ventricular cardiomyocytes. By integrating available microa…
View article: Machine learning vs. conventional statistical models for predicting heart failure readmission and mortality
Machine learning vs. conventional statistical models for predicting heart failure readmission and mortality Open
Aims This study aimed to review the performance of machine learning (ML) methods compared with conventional statistical models (CSMs) for predicting readmission and mortality in patients with heart failure (HF) and to present an approach t…