Antonia Eicher
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View article: Truncating <i>RELA</i> variants drive autoinflammation and autoimmunity by impairing the negative feedback control of NF-kB
Truncating <i>RELA</i> variants drive autoinflammation and autoimmunity by impairing the negative feedback control of NF-kB Open
The NF-κB signaling pathway coordinates inflammation, cell survival, and proliferation, while restraining excessive cell death to maintain immune homeostasis. Truncating mutations in RELA , encoding the NF-κB subunit p65, have been linked …
View article: Sample-multiplexed FACS-preprocessing of PBMCs enables scalable scRNA-seq without compromising transcriptomic or cellular integrity
Sample-multiplexed FACS-preprocessing of PBMCs enables scalable scRNA-seq without compromising transcriptomic or cellular integrity Open
Efficient preprocessing of peripheral blood mononuclear cells (PBMCs) for single-cell RNA-Sequencing (scRNA-seq) is crucial to ensure high sample throughput while maintaining sample integrity. In particular, when enrichment of rare immune …
View article: Sample-Multiplexed, FACS-Preprocessing of PBMCs with Rare Cell Type enrichment Enables Scalable scRNA-seq workflow v1
Sample-Multiplexed, FACS-Preprocessing of PBMCs with Rare Cell Type enrichment Enables Scalable scRNA-seq workflow v1 Open
This protocol describes an optimized fluorescence-activated cell sorting (FACS)–based workflow for preprocessing peripheral blood mononuclear cells (PBMCs) prior to single-cell RNA sequencing (scRNA-Seq). It enables selective enrichment of…