Antonio E. Chambers
YOU?
Author Swipe
View article: Differential Gene Expression following DHX36/G4R1 Knockout Is Associated with G-Quadruplex Content and Cancer
Differential Gene Expression following DHX36/G4R1 Knockout Is Associated with G-Quadruplex Content and Cancer Open
G-quadruplexes (G4s) are secondary DNA and RNA structures stabilized by positive cations in a central channel formed by stacked tetrads of Hoogsteen base-paired guanines. G4s form from G-rich sequences across the genome, whose biased distr…
View article: The RNA helicase DHX36/G4R1 modulates<i>C9orf72</i>GGGGCC repeat-associated translation
The RNA helicase DHX36/G4R1 modulates<i>C9orf72</i>GGGGCC repeat-associated translation Open
GGGGCC (G 4 C 2 ) hexanucleotide repeat expansions (HRE) in C9orf72 are the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Repeat-associated non-AUG (RAN) translation of this expansion g…
View article: G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells
G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells Open
G-quadruplexes (G4s) are nucleic acid structures found enriched within gene regulatory sequences. G4s control fundamental cellular processes, including replication, transcription, and translation. Proto-oncogenes are enriched with G4 seque…
View article: An <em>In Vitro</em> Assay to Detect tRNA-Isopentenyl Transferase Activity
An <em>In Vitro</em> Assay to Detect tRNA-Isopentenyl Transferase Activity Open
N6-isopentenyladenosine RNA modifications are functionally diverse and highly conserved among prokaryotes and eukaryotes. One of the most highly conserved N6-isopentenyladenosine modifications occurs at the A37 position in a subset of tRNA…
View article: Restriction digest screening facilitates efficient detection of site-directed mutations introduced by CRISPR in <i>C. albicans UME6</i>
Restriction digest screening facilitates efficient detection of site-directed mutations introduced by CRISPR in <i>C. albicans UME6</i> Open
Introduction of point mutations to a gene of interest is a powerful tool when determining protein function. CRISPR-mediated genome editing allows for more efficient transfer of a desired mutation into a wide range of model organisms. Tradi…