Antony Lechner
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View article: Structural insight into the novel <i>Thermus thermophilus</i> SPOUT methyltransferase RlmR catalysing Um2552 formation in the 23S rRNA A-loop: a case of convergent evolution
Structural insight into the novel <i>Thermus thermophilus</i> SPOUT methyltransferase RlmR catalysing Um2552 formation in the 23S rRNA A-loop: a case of convergent evolution Open
The A-loop of the 23S ribosomal RNA is a critical region of the ribosome involved in stabilizing the CCA-end of A-site-bound transfer RNA. Within this loop, nucleotide U2552 is frequently 2′-O-methylated (Um2552) in various organisms belon…
View article: Temperature-Dependent tRNA Modifications in Bacillales
Temperature-Dependent tRNA Modifications in Bacillales Open
Transfer RNA (tRNA) modifications are essential for the temperature adaptation of thermophilic and psychrophilic organisms as they control the rigidity and flexibility of transcripts. To further understand how specific tRNA modifications a…
View article: Temperature-dependent tRNA Modifications in Bacillales
Temperature-dependent tRNA Modifications in Bacillales Open
Transfer RNA (tRNA) modifications are essential for the temperature adaptation of thermophilic and psychrophilic organisms, as they control the rigidity and flexibility of transcripts. To further understand how specific tRNA modifications …
View article: The<i>Bacillus subtilis ywbD</i>gene encodes RlmQ, the 23S rRNA methyltransferase forming m<sup>7</sup>G2574 in the A-site of the peptidyl transferase center
The<i>Bacillus subtilis ywbD</i>gene encodes RlmQ, the 23S rRNA methyltransferase forming m<sup>7</sup>G2574 in the A-site of the peptidyl transferase center Open
Ribosomal RNA contains many posttranscriptionally modified nucleosides, particularly in the functional parts of the ribosome. The distribution of these modifications varies from one organism to another. In Bacillus subtilis , the model org…
View article: MODOMICS: a database of RNA modifications and related information. 2023 update
MODOMICS: a database of RNA modifications and related information. 2023 update Open
The MODOMICS database was updated with recent data and now includes new data types related to RNA modifications. Changes to the database include an expanded modification catalog, encompassing both natural and synthetic residues identified …
View article: Structure of the human 80S ribosome at 1.9 Å resolution – the molecular role of chemical modifications and ions in RNA
Structure of the human 80S ribosome at 1.9 Å resolution – the molecular role of chemical modifications and ions in RNA Open
Summary The ribosomal RNA of the human protein synthesis machinery comprises numerous chemical modifications that are introduced during ribosome biogenesis. We present the 1.9 Å resolution cryo-EM structure of the 80S human ribosome resolv…
View article: Identification of U<sup>p</sup>47 in three thermophilic archaea, one mesophilic archaeon, and one hyperthermophilic bacterium
Identification of U<sup>p</sup>47 in three thermophilic archaea, one mesophilic archaeon, and one hyperthermophilic bacterium Open
Analysis of the profile of the tRNA modifications in several Archaea allowed us to observe a novel modified uridine in the V-loop of several tRNAs from two species: Pyrococcus furiosus and Sulfolobus acidocaldarius . Recently, Ohira and co…
View article: Capillary Electrophoresis-Mass Spectrometry at Trial by Metabo-Ring: Effective Electrophoretic Mobility for Reproducible and Robust Compound Annotation
Capillary Electrophoresis-Mass Spectrometry at Trial by Metabo-Ring: Effective Electrophoretic Mobility for Reproducible and Robust Compound Annotation Open
Capillary zone electrophoresis-mass spectrometry (CE-MS) is a mature analytical tool for the efficient profiling of (highly) polar and ionizable compounds. However, the use of CE-MS in comparison to other separation techniques remains unde…
View article: Characterization of Post-Transcriptional RNA Modifications by Sheathless Capillary Electrophoresis–High Resolution Mass Spectrometry
Characterization of Post-Transcriptional RNA Modifications by Sheathless Capillary Electrophoresis–High Resolution Mass Spectrometry Open
Over the past decade there has been a growing interest in RNA modification analysis. High performance liquid chromatography-tandem mass spectrometry coupling (HPLC-MS/MS) is classically used to characterize post-transcriptional modificatio…
View article: Intact monoclonal antibodies separation and analysis by sheathless capillary electrophoresis-mass spectrometry
Intact monoclonal antibodies separation and analysis by sheathless capillary electrophoresis-mass spectrometry Open
Capillary electrophoresis-mass spectrometry coupling is a growing technique in biopharmaceutics characterization. Assessment of monoclonal antibodies is well known at middle-up and bottom-up levels to obtain information about the sequence,…