Anup K. Singh
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View article: Harmonic Summation-Based Robust Pitch Estimation in Noisy and Reverberant Environments
Harmonic Summation-Based Robust Pitch Estimation in Noisy and Reverberant Environments Open
Accurate pitch estimation is essential for numerous speech processing applications, yet it remains challenging in high-distortion environments. This paper proposes a robust pitch estimation method that delivers robust pitch estimates in ch…
View article: High-Throughput Microfluidic Electroporation (HTME): A Scalable, 384-Well Platform for Multiplexed Cell Engineering
High-Throughput Microfluidic Electroporation (HTME): A Scalable, 384-Well Platform for Multiplexed Cell Engineering Open
Electroporation-mediated gene delivery is a cornerstone of synthetic biology, offering several advantages over other methods: higher efficiencies, broader applicability, and simpler sample preparation. Yet, electroporation protocols are of…
View article: Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida
Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida Open
Advances in genome engineering have improved our ability to perturb microbial metabolic networks, yet bioproduction campaigns often struggle with parsing complex metabolic datasets to efficiently enhance product titers. We address this cha…
View article: Enzymatic cleavage of model lignin dimers depends on pH, enzyme, and bond type
Enzymatic cleavage of model lignin dimers depends on pH, enzyme, and bond type Open
Lignin is composed of phenylpropanoid monomers linked by ether and carbon-carbon bonds to form a complex heterogeneous structure. Bond-specific studies of lignin-modifying enzymes (LMEs; e.g., laccases and peroxidases) are limited by the p…
View article: Detection of Para-Bombay Phenotype Through Saliva Secretor Studies: A Critical Diagnostic Approach
Detection of Para-Bombay Phenotype Through Saliva Secretor Studies: A Critical Diagnostic Approach Open
View article: BEST-STD: Bidirectional Mamba-Enhanced Speech Tokenization for Spoken Term Detection
BEST-STD: Bidirectional Mamba-Enhanced Speech Tokenization for Spoken Term Detection Open
Spoken term detection (STD) is often hindered by reliance on frame-level features and the computationally intensive DTW-based template matching, limiting its practicality. To address these challenges, we propose a novel approach that encod…
View article: Centrifugal Microfluidic Platform for Multiplexed Detection.
Centrifugal Microfluidic Platform for Multiplexed Detection. Open
View article: Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery
Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery Open
View article: Microfluidic platform for multiplexed detection in single cells and methods thereof
Microfluidic platform for multiplexed detection in single cells and methods thereof Open
The present invention relates to a microfluidic device and platform configured to conduct multiplexed analysis within the device. In particular, the device allows multiple targets to be detected on a single-cell level. Also provided are me…
View article: Detection of active and latent infections with microfluidic devices and systems thereof
Detection of active and latent infections with microfluidic devices and systems thereof Open
The present invention relates to methods of detecting one or more targets of interest in a sample. In one instance, the target can be correlated to an active infection (e.g., by a virus and/or a bacterium). Methods can include treating the…
View article: Dielectrophoretic systems without embedded electrodes
Dielectrophoretic systems without embedded electrodes Open
Method and apparatus for dielectrophoretic separation of particles in a fluid based using array of insulating structures arranged in a fluid flow channel. By utilizing an array of insulating structures, a spatially inhomogeneous electric f…
View article: Method for forming polymerized microfluidic devices
Method for forming polymerized microfluidic devices Open
Methods for making a microfluidic device according to embodiments of the present invention include defining.about.cavity. Polymer precursor solution is positioned in the cavity, and exposed to light to begin the polymerization process and …
View article: Microfluidic platform for synthetic biology applications
Microfluidic platform for synthetic biology applications Open
This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each …
View article: Microfluidic devices, systems, and methods for quantifying particles using centrifugal force
Microfluidic devices, systems, and methods for quantifying particles using centrifugal force Open
Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluid…
View article: Devices, systems, and methods for conducting sandwich assays using sedimentation
Devices, systems, and methods for conducting sandwich assays using sedimentation Open
Embodiments of the present invention are directed toward devices, systems, and method for conducting sandwich assays using sedimentation. In one example, a method includes generating complexes on a plurality of beads in a fluid sample, ind…
View article: Dialysis on microchips using thin porous polymer membranes
Dialysis on microchips using thin porous polymer membranes Open
Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thi…
View article: Dielectrokinetic chromatography and devices thereof
Dielectrokinetic chromatography and devices thereof Open
Disclosed herein are methods and devices for dielectrokinetic chromatography. As disclosed, the devices comprise microchannels having at least one perturber which produces a non-uniformity in a field spanning the width of the microchannel.…
View article: Fluid delivery manifolds and microfluidic systems
Fluid delivery manifolds and microfluidic systems Open
Embodiments of fluid distribution manifolds, cartridges, and microfluidic systems are described herein. Fluid distribution manifolds may include an insert member and a manifold base and may define a substantially closed channel within the …
View article: Digital microfluidic systems with electrode bus and methods for droplet manipulation
Digital microfluidic systems with electrode bus and methods for droplet manipulation Open
The present disclosure relates to digital microfluidic systems having an electrode bus controlled by a single actuation input, and methods for droplet manipulation using the electrode bus. Particularly, aspects are directed to a digital mi…
View article: Devices, systems, and methods for microscale isoelectric fractionation
Devices, systems, and methods for microscale isoelectric fractionation Open
Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microflui…
View article: Method for dialysis on microchips using thin porous polymer membrane
Method for dialysis on microchips using thin porous polymer membrane Open
Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thi…
View article: Dielectrokinetic chromatography devices
Dielectrokinetic chromatography devices Open
Disclosed herein are methods and devices for dielectrokinetic chromatography. As disclosed, the devices comprise microchannels having at least one perturber which produces a non-uniformity in a field spanning the width of the microchannel.…
View article: Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis
Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis Open
Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electro…
View article: Microchannel gel electrophoretic separation systems and methods for preparing and using
Microchannel gel electrophoretic separation systems and methods for preparing and using Open
A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel e…
View article: Dialysis membrane for separation on microchips
Dialysis membrane for separation on microchips Open
Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thi…
View article: Digital microfluidic systems and methods for droplet manipulation
Digital microfluidic systems and methods for droplet manipulation Open
The present disclosure relates to digital microfluidic systems. Particularly, aspects are directed to a digital microfluidic system that includes a droplet chip having a substrate, a plurality of electrodes and corresponding plurality of c…
View article: Microfluidic devices and methods including porous polymer monoliths
Microfluidic devices and methods including porous polymer monoliths Open
Microfluidic devices and methods including porous polymer monoliths are described. Polymerization techniques may be used to generate porous polymer monoliths having pores defined by a liquid component of a fluid mixture. The fluid mixture …
View article: Perspectives for self-driving labs in synthetic biology
Perspectives for self-driving labs in synthetic biology Open
Self-driving labs (SDLs) combine fully automated experiments with artificial intelligence (AI) that decides the next set of experiments. Taken to their ultimate expression, SDLs could usher a new paradigm of scientific research, where the …
View article: Development of Engineered Candida Tropicalis Strain for Efficient Corncob-Based Xylitol-Ethanol Biorefinery
Development of Engineered Candida Tropicalis Strain for Efficient Corncob-Based Xylitol-Ethanol Biorefinery Open
View article: Attention-based audio embeddings for query-by-example
Attention-based audio embeddings for query-by-example Open
An ideal audio retrieval system efficiently and robustly recognizes a short query snippet from an extensive database. However, the performance of well-known audio fingerprinting systems falls short at high signal distortion levels. This pa…