Arthur Hinterholzer
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View article: Unambiguous Identification of Glucose-Induced Glycation in mAbs and other Proteins by NMR Spectroscopy
Unambiguous Identification of Glucose-Induced Glycation in mAbs and other Proteins by NMR Spectroscopy Open
Objective Glycation is a non-enzymatic and spontaneous post-translational modification (PTM) generated by the reaction between reducing sugars and primary amine groups within proteins. Because glycation can alter the properties of proteins…
View article: Unambiguous identification of α-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy
Unambiguous identification of α-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy Open
The α-Gal epitope consisting of the terminal trisaccharide Galα1,3Galβ1,4GlcNAc exposed on cell or protein surfaces can cause severe immune reactions, such as hypersensitivity reactions, in humans. This epitope is also called the xenotrans…
View article: Backbone distortions in lactam‐bridged helical peptides
Backbone distortions in lactam‐bridged helical peptides Open
Side‐chain‐to‐side‐chain cyclization is frequently used to stabilize the α‐helical conformation of short peptides. In a previous study, we incorporated a lactam bridge between the side chains of Lys‐i and Asp‐i+4 in the nonapeptide 1Y , cy…
View article: A Conformationally Stable Acyclic β‐Hairpin Scaffold Tolerating the Incorporation of Poorly β‐Sheet‐Prone Amino Acids
A Conformationally Stable Acyclic β‐Hairpin Scaffold Tolerating the Incorporation of Poorly β‐Sheet‐Prone Amino Acids Open
The β‐hairpin is a structural element of native proteins, but it is also a useful artificial scaffold for finding lead compounds to convert into peptidomimetics or non‐peptide structures for drug discovery. Since linear peptides are synthe…
View article: Detecting aspartate isomerization and backbone cleavage after aspartate in intact proteins by NMR spectroscopy
Detecting aspartate isomerization and backbone cleavage after aspartate in intact proteins by NMR spectroscopy Open
The monitoring of non-enzymatic post-translational modifications (PTMs) in therapeutic proteins is important to ensure drug safety and efficacy. Together with methionine and asparagine, aspartic acid (Asp) is very sensitive to spontaneous …
View article: Identification and Quantification of Oxidation Products in Full-Length Biotherapeutic Antibodies by NMR Spectroscopy
Identification and Quantification of Oxidation Products in Full-Length Biotherapeutic Antibodies by NMR Spectroscopy Open
Therapeutic proteins are an indispensable class of drugs and often therapeutics of last resort. They are sensitive to oxidation, which is of critical concern, because it can affect drug safety and efficacy. Protein oxidation, with methioni…
View article: Fold stability during endolysosomal acidification is a key factor for allergenicity and immunogenicity of the major birch pollen allergen
Fold stability during endolysosomal acidification is a key factor for allergenicity and immunogenicity of the major birch pollen allergen Open
Our data indicate that differential pH-dependent fold stability along endosomal maturation is an essential protein-inherent determinant of allergenicity.