Aset Khakimzhan
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View article: Large-scale-integration and collective oscillations of 2D artificial cells
Large-scale-integration and collective oscillations of 2D artificial cells Open
The on-chip large-scale-integration of genetically programmed artificial cells capable of exhibiting collective expression patterns is important for fundamental research and biotechnology. Here, we report a 3D biochip with a 2D layout of 1…
View article: Red Light Mediated Photo-Conversion of Silicon Rhodamines to Oxygen Rhodamines for Single-Molecule Microscopy
Red Light Mediated Photo-Conversion of Silicon Rhodamines to Oxygen Rhodamines for Single-Molecule Microscopy Open
The rhodamine motif has been modified in myriad ways to produce probes with specific fluorescent and chemical properties optimal for a variety of microscopy experiments. Recently, far-red emitting silicon rhodamines have become popular in …
View article: Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins
Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins Open
Integral and interacting membrane proteins (IIMPs) constitute a vast family of biomolecules that perform essential functions in all forms of life. However, characterizing their interactions with lipid bilayers remains limited due to challe…
View article: On-chip large-scale-integration and 2D collective modes of genetically programmed artificial cells
On-chip large-scale-integration and 2D collective modes of genetically programmed artificial cells Open
The on-chip large-scale-integration of genetically programmed artificial cells capable of exhibiting collective modes is an important goal for fundamental research and technology. Here, we report assembly of a 2D layout of 1024 monolithic …
View article: Critical role of DNA unwinding and Cas9 conformational changes in modelling off-target effects
Critical role of DNA unwinding and Cas9 conformational changes in modelling off-target effects Open
CRISPR-Cas9 off-target effects interfere with the ability to accurately perform genetic edits. To predict off-target effects CRISPR-Cas9 researchers perform high throughput guide RNA mismatch and bulge experiments and then use the data to …
View article: The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform
The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform Open
The new generation of cell-free gene expression systems enables the prototyping and engineering of biological systems in vitro over a remarkable scope of applications and physical scales. As the utilization of DNA-directed in vitro protein…
View article: Proofreading mechanism of Class 2 CRISPR-Cas systems
Proofreading mechanism of Class 2 CRISPR-Cas systems Open
CRISPR systems experience off-target effects that interfere with the ability to accurately perform genetic edits. While empirical models predict off-target effects in specific platforms, there is a gap for a wide-ranging mechanistic model …