Explanipedia

Deletion of KLHDC3, an E3 ubiquitin ligase complex substrate receptor, leads to obesity in mice Open

Paula Armina V. Buco, Ashfaqul Hoque, Wilson Castillo‐Tandazo, Alistair M. Chalk, Monique Smeets , et al. · 2025

Protein ubiquitination is a critical post-translational modification that regulates protein stability and cellular homeostasis. KLHDC3 is a substrate recognition receptor within the recently identified C-terminal degron-mediated DesCEND ub…

Comparison of Classical P81 and SVI-P Cation-Exchange Papers in Radiometric Protein Kinase Assays Open

Muhammad Khabib, Lifang Zhang, Ashley J. Ovens, Abdulhameed Al‐Ghabkari, John W. Scott , et al. · 2025

Radiometric kinase assays have been widely used due to their high sensitivity and dynamic range. The assay measures the transfer of 32Pi from [γ-32P]-ATP to specific substrates, typically synthetic peptides. The 32P-phosphorylated peptide …

Enhanced Expression of Mitochondrial Magmas Protein in Ovarian Carcinomas: Magmas Inhibition Facilitates Antitumour Effects, Signifying a Novel Approach for Ovarian Cancer Treatment Open

Ali Raza, Ashfaqul Hoque, Rodney B. Luwor, Ruth M. Escalona, Jason Kelly , et al. · 2025

Mitochondrial-associated granulocyte macrophage colony-stimulating factor (Magmas) is a unique protein located in the inner membrane of mitochondria, with an active role in scavenging reactive oxygen species (ROS) in cellular systems. Ovar…

AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates Open

William J. Smiles, Ashley J. Ovens, Dingyi Yu, Naomi X.Y. Ling, Andrea C. Poblete Goycoolea , et al. · 2025

N-Terminomic Changes in Neurons During Excitotoxicity Reveal Proteolytic Events Associated With Synaptic Dysfunctions and Potential Targets for Neuroprotection Open

S. Sadia Ameen, Nane Griem‐Krey, Antoine Dufour, Mohammed Iqbal Hossain, Ashfaqul Hoque , et al. · 2023

Excitotoxicity, a neuronal death process in neurological disorders such as stroke, is initiated by the overstimulation of ionotropic glutamate receptors. Although dysregulation of proteolytic signaling networks is critical for excitotoxici…

Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis Open

Katyayanee Neopane, Natalie Kozlov, Florentina Negoita, Lisa Murray‐Segal, Robert Brink , et al. · 2022

AMP-activated protein kinase (AMPK) is a master regulator of cellular energy homeostasis and a therapeutic target for metabolic diseases. Co/post-translational N-myristoylation of glycine-2 (Gly2) of the AMPK β subunit has been suggested t…

N-Terminomic Changes of Neurons During Excitotoxicity Reveal Proteolytic Events Associated with Synaptic Dysfunctions and Inform Potential Targets for Neuroprotection Open

S. Sadia Ameen, Nane Griem‐Krey, Antoine Dufour, Mohammed Iqbal Hossain, Ashfaqul Hoque , et al. · 2022

Excitotoxicity is a neuronal death process initiated by over-stimulation of ionotropic glutamate receptors. Although dysregulation of proteolytic signaling networks is critical for excitotoxicity, the identity of affected proteins and mech…

An AMPKα2-specific phospho-switch controls lysosomal targeting for activation Open

Kaitlin R. Morrison, William J. Smiles, Naomi X.Y. Ling, Ashfaqul Hoque, Gabrielle Shea , et al. · 2022

AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) are metabolic kinases that co-ordinate nutrient supply with cell growth. AMPK negatively regulates mTORC1, and mTORC1 reciprocally phosphorylates S3…

Novel torin1-sensitive phosphorylation sites on the metabolic regulator AMPK revealed by label-free mass spectrometry Open

William J. Smiles, Ashley J. Ovens, Dingyi Yu, Naomi X.Y. Ling, Kaitlin R. Morrison , et al. · 2021

AMPK and mTORC1 are nutrient-sensitive protein kinases that form a fundamental negative feedback loop that governs cell growth and proliferation. AMPK is an αβγ heterotrimer that is directly phosphorylated by mTORC1 on α2 S345 to suppress …

CaMKK2 is inactivated by cAMP-PKA signaling and 14-3-3 adaptor proteins Open

Christopher G. Langendorf, Matthew T. O’Brien, Kevin R. W. Ngoei, Luke M. McAloon, Urmi Dhagat , et al. · 2020

Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms Open

Stephen L. Pinkosky, John W. Scott, Eric M. Desjardins, Brennan K. Smith, Emily A. Day , et al. · 2020

An Atlas of Phosphorylation and Proteolytic Processing Events During Excitotoxic Neuronal Death Reveals New Therapeutic Opportunities Open

S. Sadia Ameen, Antoine Dufour, Mohammed Iqbal Hossain, Ashfaqul Hoque, Sharelle A. Sturgeon , et al. · 2020

Summary Excitotoxicity, a neuronal death process in neurological disorders, is initiated by over-stimulation of neuronal ionotropic glutamate receptors. The over-stimulated receptors dysregulate proteases, protein kinases and phosphatases,…

Functional analysis of an R311C variant of Ca<sup>2+</sup>‐calmodulin‐dependent protein kinase kinase‐2 (CaMKK2) found as a de novo mutation in a patient with bipolar disorder Open

Naomi X.Y. Ling, Christopher G. Langendorf, Ashfaqul Hoque, Sandra Galić, Kim Loh , et al. · 2020

Objectives Loss‐of‐function mutations in the gene encoding the calcium‐calmodulin (Ca 2+ ‐CaM)‐dependent protein kinase kinase‐2 (CaMKK2) enzyme are linked to bipolar disorder. Recently, a de novo arginine to cysteine (R311C) mutation in C…

The eEF2 kinase-induced STAT3 inactivation inhibits lung cancer cell proliferation by phosphorylation of PKM2 Open

Min Xiao, Jianling Xie, Yu Wu, Genzhu Wang, Xin Qi , et al. · 2020

mTORC1 directly inhibits AMPK to promote cell proliferation under nutrient stress Open

Naomi X.Y. Ling, Adrian Kaczmarek, Ashfaqul Hoque, Elizabeth A. Colby Davie, Kevin R. W. Ngoei , et al. · 2020

Additional file 1 of The eEF2 kinase-induced STAT3 inactivation inhibits lung cancer cell proliferation by phosphorylation of PKM2 Open

Min Xiao, Jianling Xie, Yu Wu, Genzhu Wang, Xin Qi , et al. · 2020

Additional file 1: Figure S1. The phosphorylation level and activity of eEF2K in A549 when cultured with glucose-free or serum-free mediun. Figure S2. eEF2K inhibition by A484954 alleviates the effects of gefitinib. Figure S3. eEF2K positi…

Construction and reconstruction of brain circuits: normal and pathological axon guidance Open

Sergi Roig‐Puiggros, Robin Vigouroux, Danielle Beckman, Nadia I. Bocai, Brian Chiou , et al. · 2019

Perception of our environment entirely depends on the close interaction between the central and peripheral nervous system. In order to communicate each other, both systems must develop in parallel and in coordination. During development, a…

Quantitative proteomic analyses of dynamic signalling events in cortical neurons undergoing excitotoxic cell death Open

Ashfaqul Hoque, Nicholas A. Williamson, S. Sadia Ameen, Giuseppe D. Ciccotosto, Mohammed Iqbal Hossain , et al. · 2019

AMP-activated protein kinase selectively inhibited by the type II inhibitor SBI-0206965 Open

Toby A. Dite, Christopher G. Langendorf, Ashfaqul Hoque, Sandra Galić, Richard J. Rebello , et al. · 2018

Inhibition of the metabolic regulator AMP-activated protein kinase (AMPK) is increasingly being investigated for its therapeutic potential in diseases where AMPK hyperactivity results in poor prognoses, as in established cancers and neurod…

Structural Determinants for Small-Molecule Activation of Skeletal Muscle AMPK α2β2γ1 by the Glucose Importagog SC4 Open

Kevin R. W. Ngoei, Christopher G. Langendorf, Naomi X.Y. Ling, Ashfaqul Hoque, Swapna Varghese , et al. · 2018

Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells Open

Ashfaqul Hoque, Priyadharshini Sivakumaran, Simon T. Bond, Naomi X.Y. Ling, Anne M. Kong , et al. · 2018

The autophagy initiator ULK1 sensitizes AMPK to allosteric drugs Open

Toby A. Dite, Naomi X.Y. Ling, John W. Scott, Ashfaqul Hoque, Sandra Galić , et al. · 2017

A proteomic characterization shows differences in the milk fat globule membrane of buffalo and bovine milk Open

Hanh T.H. Nguyen, Lydia Ong, Ashfaqul Hoque, Sandra E. Kentish, Nicholas A. Williamson , et al. · 2017

Impact of Genetic Variation on Human CaMKK2 Regulation by Ca2+-Calmodulin and Multisite Phosphorylation Open

Matthew T. O’Brien, Jonathan S. Oakhill, Naomi X.Y. Ling, Christopher G. Langendorf, Ashfaqul Hoque , et al. · 2017

Exploring the signalling mechanism of excitotoxic neuronal injury by molecular and quantitative proteomic approaches Open

Ashfaqul Hoque · 2016

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