Ashutosh Wadhwa
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View article: Comparing the genetic typing methods for effective surveillance and rabies control in Georgia
Comparing the genetic typing methods for effective surveillance and rabies control in Georgia Open
A full nucleoprotein gene sequencing of 68 isolates collected from passive rabies surveillance system in Georgia between 2015 and 2016 identified two distinct dog rabies phylogroups, GEO_V1 and GEO_V2, which both belonged to the cosmopolit…
View article: Performance of existing and novel surveillance case definitions for COVID-19 in household contacts of PCR-confirmed COVID-19
Performance of existing and novel surveillance case definitions for COVID-19 in household contacts of PCR-confirmed COVID-19 Open
Background Optimized symptom-based COVID-19 case definitions that guide public health surveillance and individual patient management in the community may assist pandemic control. Methods We assessed diagnostic performance of existing cases…
View article: Comparison of the SARS-CoV-2 spike protein ELISA and the Abbott Architect SARS-CoV-2 IgG nucleocapsid protein assays for detection of antibodies
Comparison of the SARS-CoV-2 spike protein ELISA and the Abbott Architect SARS-CoV-2 IgG nucleocapsid protein assays for detection of antibodies Open
Serologic assays developed for SARS-CoV-2 detect different antibody subtypes and are based on different target antigens. Comparison of the performance of a SARS-CoV-2 Spike-Protein ELISA and the nucleocapsid-based Abbott Architect TM SARS-…
View article: Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Surface water (and other types of water samples) v1
Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Surface water (and other types of water samples) v1 Open
The protocol describes method for qualitative detection (presence/ absence) of Salmonella Typhi and Salmonella Paratyphi A in surface water by enrichment culture followed by real-time PCR. The same protocol can be used for other types of w…
View article: Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Soil v1
Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Soil v1 Open
The protocol describes method for qualitative detection (presence/ absence) of Salmonella Typhi and Salmonella Paratyphi A in soil by enrichment culture followed by real-time PCR.
View article: Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Produce v1
Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Produce v1 Open
The protocol describes method for qualitative detection (presence/ absence) of Salmonella Typhi and Salmonella Paratyphi A on produce by enrichment culture followed by real-time PCR.
View article: Evaluation of Low-Cost Phage-Based Microbial Source Tracking Tools for Elucidating Human Fecal Contamination Pathways in Kolkata, India
Evaluation of Low-Cost Phage-Based Microbial Source Tracking Tools for Elucidating Human Fecal Contamination Pathways in Kolkata, India Open
Graphical Abstract Phages infecting Bacteroides fragilis strain GB-124 and non-source specific somatic coliphages (SOMCPH) were deployed for identification of fecal contamination pathways in Kolkata, India. Analyses of environmental sample…
View article: Azithromycin and Ciprofloxacin Treatment Outcomes During an Outbreak of Multidrug-Resistant <i>Shigella sonnei</i> Infections in a Retirement Community—Vermont, 2018
Azithromycin and Ciprofloxacin Treatment Outcomes During an Outbreak of Multidrug-Resistant <i>Shigella sonnei</i> Infections in a Retirement Community—Vermont, 2018 Open
Background In 2018, the Centers for Disease Control and Prevention and the Vermont Department of Health investigated an outbreak of multidrug-resistant Shigella sonnei infections in a retirement community that offered a continuum of care f…
View article: Persistent SARS-CoV-2 RNA Shedding Without Evidence of Infectiousness: A Cohort Study of Individuals With COVID-19
Persistent SARS-CoV-2 RNA Shedding Without Evidence of Infectiousness: A Cohort Study of Individuals With COVID-19 Open
Background To better understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding and infectivity, we estimated SARS-CoV-2 RNA shedding duration, described participant characteristics associated with the first negative …
View article: Identification of Presymptomatic and Asymptomatic Cases Using Cohort-Based Testing Approaches at a Large Correctional Facility—Chicago, Illinois, USA, May 2020
Identification of Presymptomatic and Asymptomatic Cases Using Cohort-Based Testing Approaches at a Large Correctional Facility—Chicago, Illinois, USA, May 2020 Open
Background Coronavirus disease 2019 (COVID-19) continues to cause significant morbidity and mortality worldwide. Correctional and detention facilities are at high risk of experiencing outbreaks. We aimed to evaluate cohort-based testing am…
View article: Improving Confirmatory Testing for the Antimicrobial Resistance Surveillance Network in Ethiopia
Improving Confirmatory Testing for the Antimicrobial Resistance Surveillance Network in Ethiopia Open
Background: In July 2017, the Ethiopian Public Health Institute (EPHI) launched an antimicrobial resistance (AMR) surveillance network at 4 sentinel laboratories. The National Clinical Bacteriology and Mycology Laboratory (NRL) at EPHI per…
View article: Epidemiological Correlates of Polymerase Chain Reaction Cycle Threshold Values in the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Epidemiological Correlates of Polymerase Chain Reaction Cycle Threshold Values in the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Open
Background Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has principally been performed through the use of real-time reverse-transcription polymerase chain reaction testing. Results of such tests can b…
View article: Household Transmission of Severe Acute Respiratory Syndrome Coronavirus-2 in the United States
Household Transmission of Severe Acute Respiratory Syndrome Coronavirus-2 in the United States Open
Background The evidence base for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is nascent. We sought to characterize SARS-CoV-2 transmission within US households and estimate the household secondary infection rate (SIR) to i…
View article: A Prospective Cohort Study in Nonhospitalized Household Contacts With Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Symptom Profiles and Symptom Change Over Time
A Prospective Cohort Study in Nonhospitalized Household Contacts With Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Symptom Profiles and Symptom Change Over Time Open
Background Improved understanding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spectrum of disease is essential for clinical and public health interventions. There are limited data on mild or asymptomatic infections, but…
View article: Designing a typhoid environmental surveillance study: A simulation model for optimum sampling site allocation
Designing a typhoid environmental surveillance study: A simulation model for optimum sampling site allocation Open
View article: Characterization of Ethanol Extracted Cell Wall Components of Mycobacterium avium Subsp. paratuberculosis
Characterization of Ethanol Extracted Cell Wall Components of Mycobacterium avium Subsp. paratuberculosis Open
Antigens extracted using ethanol (EtOH) and incorporated in the EtOH vortex ELISA (EVELISA) test have previously shown high specificity and sensitivity for detecting Mycobacterium avium subspecies paratuberculosis (Map) and M. bovis infect…
View article: 1603. Observation of Treatment Outcomes During an Outbreak of Multidrug-Resistant Shigella sonnei Infections in a Retirement Community—Vermont, 2018
1603. Observation of Treatment Outcomes During an Outbreak of Multidrug-Resistant Shigella sonnei Infections in a Retirement Community—Vermont, 2018 Open
Background In 2018, CDC and the Vermont Department of Health investigated an outbreak of multidrug-resistant Shigella sonnei infections in a retirement community. Most Shigella infections are self-limited, but antibiotics are indicated for…
View article: <i>Notes from the Field:</i> Outbreak of Multidrug-Resistant <i>Shigella sonnei</i> Infections in a Retirement Community — Vermont, October–November 2018
<i>Notes from the Field:</i> Outbreak of Multidrug-Resistant <i>Shigella sonnei</i> Infections in a Retirement Community — Vermont, October–November 2018 Open
View article: Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics
Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics Open
Rabies is a fatal zoonotic disease that requires fast, accurate diagnosis to prevent disease in an exposed individual. The current gold standard for post-mortem diagnosis of human and animal rabies is the direct fluorescent antibody (DFA) …
View article: LN34 real-time RT-PCR protocol v1
LN34 real-time RT-PCR protocol v1 Open
Purpose To describe the LN34 real-time RT-PCR assay procedure used for the qualitative detection of lyssavirus RNA in whole RNA extracted from brain tissue samples. The assay detects RNA from diverse lyssaviruses at varying concentrations …
View article: LN34 real-time RT-PCR protocol v2
LN34 real-time RT-PCR protocol v2 Open
Purpose To describe the LN34 real-time RT-PCR assay procedure used for the qualitative detection of lyssavirus RNA in whole RNA extracted from brain tissue samples. The assay detects RNA from diverse lyssaviruses at varying concentrations …
View article: A Pan-Lyssavirus Taqman Real-Time RT-PCR Assay for the Detection of Highly Variable Rabies virus and Other Lyssaviruses
A Pan-Lyssavirus Taqman Real-Time RT-PCR Assay for the Detection of Highly Variable Rabies virus and Other Lyssaviruses Open
Rabies, resulting from infection by Rabies virus (RABV) and related lyssaviruses, is one of the most deadly zoonotic diseases and is responsible for up to 70,000 estimated human deaths worldwide each year. Rapid and accurate laboratory dia…
View article: Human Rabies — Wyoming and Utah, 2015
Human Rabies — Wyoming and Utah, 2015 Open
In September 2015, a Wyoming woman was admitted to a local hospital with a 5-day history of progressive weakness, ataxia, dysarthria, and dysphagia. Because of respiratory failure, she was transferred to a referral hospital in Utah, where …
View article: Human Rabies — Missouri, 2014
Human Rabies — Missouri, 2014 Open
On September 18, 2014, the Missouri Department of Health and Senior Services (MDHSS) was notified of a suspected rabies case in a Missouri resident. The patient, a man aged 52 years, lived in a rural, deeply wooded area, and bat sightings …