Askar A. Kleefeldt
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View article: <i>Escherichia coli</i> with a 57-codon genetic code
<i>Escherichia coli</i> with a 57-codon genetic code Open
The near-universal genetic code uses 64 codons to encode the 20 canonical amino acids and protein synthesis. Here, we designed and generated Escherichia coli with a 4-megabase synthetic genome in which we replaced known occurrences of six …
View article: Genetic Code-Locking Confers Stable Virus Resistance to a Recoded Organism
Genetic Code-Locking Confers Stable Virus Resistance to a Recoded Organism Open
The genetic code defines the correspondence between codons in genes and amino acids in proteins. Reassignment of sense codons to different amino acids can create cells with refactored genetic codes that are distinct from the canonical gene…
View article: <i>Escherichia coli</i> with a 57-codon genetic code
<i>Escherichia coli</i> with a 57-codon genetic code Open
The near-universal genetic code of living organisms uses 64 codons to encode the 20 canonical amino acids in protein synthesis. Here we design and generate a variant of Escherichia coli with a 4 Mb synthetic genome in which we replace ever…
View article: Long‐read direct RNA sequencing of the mitochondrial transcriptome of <i>Saccharomyces cerevisiae</i> reveals condition‐dependent intron abundance
Long‐read direct RNA sequencing of the mitochondrial transcriptome of <i>Saccharomyces cerevisiae</i> reveals condition‐dependent intron abundance Open
Mitochondria fulfil many essential roles and have their own genome, which is expressed as polycistronic transcripts that undergo co‐ or posttranscriptional processing and splicing. Due to the inherent complexity and limited technical acces…
View article: Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly
Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly Open
View article: Long-read direct RNA sequencing of the mitochondrial transcriptome of<i>Saccharomyces cerevisiae</i>reveals condition-dependent intron turnover
Long-read direct RNA sequencing of the mitochondrial transcriptome of<i>Saccharomyces cerevisiae</i>reveals condition-dependent intron turnover Open
Mitochondria fulfil many essential roles and have their own genome, which is expressed as polycistronic transcripts that undergo co- or post-transcriptional processing and splicing. Due to inherent complexity and limited technical accessib…
View article: The functional landscape of the human phosphoproteome
The functional landscape of the human phosphoproteome Open
View article: The functional landscape of the human phosphoproteome
The functional landscape of the human phosphoproteome Open
Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. While tens of thousands of phosphorylation sites have been identified in human cells to date, the extent and fun…