Assaf Alon
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View article: Structures of the σ2 receptor enable docking for bioactive ligand discovery
Structures of the σ2 receptor enable docking for bioactive ligand discovery Open
The σ2 receptor has attracted intense interest in cancer imaging1, psychiatric disease2, neuropathic pain3–5 and other areas of biology6,7. Here we determined the crystal structure of this receptor in complex with the clinical candidate ro…
View article: COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms
COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms Open
SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. SARS-CoV-2 infection is necessary but not sufficient for development of clinical COVID-19 disease. Currently, t…
View article: AlphaFold2 structures guide prospective ligand discovery
AlphaFold2 structures guide prospective ligand discovery Open
AlphaFold2 (AF2) models have had wide impact but mixed success in retrospective ligand recognition. We prospectively docked large libraries against unrefined AF2 models of the σ 2 and serotonin 2A (5-HT2A) receptors, testing hundreds of ne…
View article: AlphaFold2 structures template ligand discovery
AlphaFold2 structures template ligand discovery Open
AlphaFold2 (AF2) and RosettaFold have greatly expanded the number of structures available for structure-based ligand discovery, even though retrospective studies have cast doubt on their direct usefulness for that goal. Here, we tested unr…
View article: Antagonistic nanobodies reveal mechanism of GSDMD pore formation and unexpected therapeutic potential
Antagonistic nanobodies reveal mechanism of GSDMD pore formation and unexpected therapeutic potential Open
Activation of various inflammasomes converges on the cleavage of gasdermin D (GSDMD) by pro-inflammatory caspases, followed by oligomerization of the N-terminal domain (GSDMD NT ) and the assembly of pores penetrating target membranes. Yet…
View article: The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination
The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination Open
In response to starvation, endospore-forming bacteria differentiate into stress-resistant spores that can remain dormant for years yet rapidly germinate and resume growth in response to nutrients. The small molecule dipicolinic acid (DPA) …
View article: [RETRACTED] Pure Botanical CBD Gummies - Well Known At This Point? v1
[RETRACTED] Pure Botanical CBD Gummies - Well Known At This Point? v1 Open
[RETRACTED] Wheatgrass is often used by consumers who want totrigger weight loss and reduce inflammation.It is known for many health benefits
View article: [RETRACTED] Pure Botanical CBD Gummies - Well Known At This Point? v1
[RETRACTED] Pure Botanical CBD Gummies - Well Known At This Point? v1 Open
[RETRACTED] Wheatgrass is often used by consumers who want totrigger weight loss and reduce inflammation.It is known for many health benefits
View article: [RETRACTED] Lights Out CBD Gummies - Fake Hype Brand or High Quality? v1
[RETRACTED] Lights Out CBD Gummies - Fake Hype Brand or High Quality? v1 Open
[RETRACTED] it can be difficult to sift through all the information and pick out what’s truly useful.
View article: [RETRACTED] Lights Out CBD Gummies - Fake Hype Brand or High Quality? v1
[RETRACTED] Lights Out CBD Gummies - Fake Hype Brand or High Quality? v1 Open
[RETRACTED] it can be difficult to sift through all the information and pick out what’s truly useful.
View article: Dormant spores sense amino acids through the B subunits of their germination receptors
Dormant spores sense amino acids through the B subunits of their germination receptors Open
Bacteria from the orders Bacillales and Clostridiales differentiate into stress-resistant spores that can remain dormant for years, yet rapidly germinate upon nutrient sensing. How spores monitor nutrients is poorly understood but in most …
View article: The spliced leader RNA silencing (SLS) pathway in <i>Trypanosoma brucei</i> is induced by perturbations of endoplasmic reticulum, Golgi, or mitochondrial proteins factors and functional analysis of SLS inducing kinase, PK3
The spliced leader RNA silencing (SLS) pathway in <i>Trypanosoma brucei</i> is induced by perturbations of endoplasmic reticulum, Golgi, or mitochondrial proteins factors and functional analysis of SLS inducing kinase, PK3 Open
In the parasite Trypanosoma brucei , the causative agent of human African sleeping sickness, all mRNAs are trans -spliced to generate a common 5’ exon derived from the spliced leader RNA (SL RNA). Perturbations of protein translocation acr…
View article: Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2
Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 Open
Screening for drugs that don’t work In the battle against COVID-19, drugs discovered in repurposing screens are of particular interest because these could be rapidly implemented as treatments. However, Tummino et al . deliver a cautionary …
View article: Crystal structures of the σ<sub>2</sub>receptor template large-library docking for selective chemotypes active<i>in vivo</i>
Crystal structures of the σ<sub>2</sub>receptor template large-library docking for selective chemotypes active<i>in vivo</i> Open
The σ 2 receptor is a poorly understood transmembrane receptor that has attracted intense interest in many areas of biology including cancer imaging, Alzheimer’s disease, schizophrenia, and neuropathic pain. However, little is known regard…
View article: Phospholipidosis is a shared mechanism underlying the<i>in vitro</i>antiviral activity of many repurposed drugs against SARS-CoV-2
Phospholipidosis is a shared mechanism underlying the<i>in vitro</i>antiviral activity of many repurposed drugs against SARS-CoV-2 Open
Repurposing drugs as treatments for COVID-19 has drawn much attention. A common strategy has been to screen for established drugs, typically developed for other indications, that are antiviral in cells or organisms. Intriguingly, most of t…
View article: COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms
COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms Open
SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. SARS-CoV-2 infection is necessary but not sufficient for development of clinical COVID-19 disease. Currently, t…
View article: COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms
COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms Open
SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. Currently, there are no pre- or post-exposure prophylactic COVID-19 medical countermeasures. Clinical data sugg…
View article: COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms
COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms Open
SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. Currently, there are no pre- or post-exposure prophylactic COVID-19 medical countermeasures. Clinical data sugg…
View article: COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms
COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms Open
SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. Currently, there are no pre- or post-exposure prophylactic COVID-19 medical countermeasures. Clinical data sugg…
View article: <i>cis</i>‐Proline mutants of quiescin sulfhydryl oxidase 1 with altered redox properties undermine extracellular matrix integrity and cell adhesion in fibroblast cultures
<i>cis</i>‐Proline mutants of quiescin sulfhydryl oxidase 1 with altered redox properties undermine extracellular matrix integrity and cell adhesion in fibroblast cultures Open
The thioredoxin superfamily has expanded and diverged extensively throughout evolution such that distant members no longer show appreciable sequence homology. Nevertheless, redox‐active thioredoxin‐fold proteins functioning in diverse phys…
View article: Identification of the gene that codes for the σ<sub>2</sub>receptor
Identification of the gene that codes for the σ<sub>2</sub>receptor Open
Significance Of the many receptors that were pharmacologically described during the 20th century, almost all were cloned by the end of the 1990s. A key exception is the σ 2 receptor, a potential therapeutic target for diseases as diverse a…
View article: <i>AbDesign</i>: An algorithm for combinatorial backbone design guided by natural conformations and sequences
<i>AbDesign</i>: An algorithm for combinatorial backbone design guided by natural conformations and sequences Open
Computational design of protein function has made substantial progress, generating new enzymes, binders, inhibitors, and nanomaterials not previously seen in nature. However, the ability to design new protein backbones for function--essent…