Aswin Mangerich
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View article: Fluoride intake during pregnancy: calculation of realistic exposure scenarios for individual risk assessment
Fluoride intake during pregnancy: calculation of realistic exposure scenarios for individual risk assessment Open
Using the methodological approach of systematic review several regulatory bodies and associations have performed an assessment of existing evidence on the adverse effects of fluoride. The most recent review by EFSA published in 2025 recomm…
View article: The long way from raw data to NAM-based information: Overview on data layers and processing steps
The long way from raw data to NAM-based information: Overview on data layers and processing steps Open
Toxicological test methods generate raw data and provide instructions on how to use these to determine a final outcome such as a classification of test compounds as hits or non-hits. The data processing pipeline provided in the test method…
View article: Monitoring nucleolar-nucleoplasmic protein shuttling in living cells by high-content microscopy and automated image analysis
Monitoring nucleolar-nucleoplasmic protein shuttling in living cells by high-content microscopy and automated image analysis Open
The nucleolus has core functions in ribosome biosynthesis, but also acts as a regulatory hub in a plethora of non-canonical processes, including cellular stress. Upon DNA damage, several DNA repair factors shuttle between the nucleolus and…
View article: Life-Cycle-Dependent Toxicities of Mono- and Bifunctional Alkylating Agents in the 3R-Compliant Model Organism C. elegans
Life-Cycle-Dependent Toxicities of Mono- and Bifunctional Alkylating Agents in the 3R-Compliant Model Organism C. elegans Open
Caenorhabditis elegans (C. elegans) is gaining recognition and importance as an organismic model for toxicity testing in line with the 3Rs principle (replace, reduce, refine). In this study, we explored the use of C. elegans to examine the…
View article: NAD+ Acts as a Protective Factor in Cellular Stress Response to DNA Alkylating Agents
NAD+ Acts as a Protective Factor in Cellular Stress Response to DNA Alkylating Agents Open
Sulfur mustard (SM) and its derivatives are potent genotoxic agents, which have been shown to trigger the activation of poly (ADP-ribose) polymerases (PARPs) and the depletion of their substrate, nicotinamide adenine dinucleotide (NAD+). N…
View article: Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development
Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development Open
Poly(ADP-ribose) polymerase-1 (PARP1) binds DNA lesions to catalyse poly(ADP-ribosyl)ation (PARylation) using NAD+ as a substrate. PARP1 plays multiple roles in cellular activities, including DNA repair, transcription, cell death, and chro…
View article: Influence of chain length and branching on poly(ADP-ribose)–protein interactions
Influence of chain length and branching on poly(ADP-ribose)–protein interactions Open
Hundreds of proteins interact with poly(ADP-ribose) (PAR) via multiple PAR interaction motifs, thereby regulating their physico-chemical properties, sub-cellular localizations, enzymatic activities, or protein stability. Here, we present a…
View article: Comparative analysis of chlorambucil-induced DNA lesion formation and repair in a spectrum of different human cell systems
Comparative analysis of chlorambucil-induced DNA lesion formation and repair in a spectrum of different human cell systems Open
Chlorambucil (CLB) belongs to the class of nitrogen mustards (NMs), which are highly reactive bifunctional alkylating agents and were the first chemotherapeutic agents developed. They form DNA interstrand crosslinks (ICLs), which cause a b…
View article: The C-Terminal Domain of Y-Box Binding Protein 1 Exhibits Structure-Specific Binding to Poly(ADP-Ribose), Which Regulates PARP1 Activity
The C-Terminal Domain of Y-Box Binding Protein 1 Exhibits Structure-Specific Binding to Poly(ADP-Ribose), Which Regulates PARP1 Activity Open
Y-box-binding protein 1 (YB-1) is a multifunctional protein involved in the regulation of gene expression. Recent studies showed that in addition to its role in the RNA and DNA metabolism, YB-1 is involved in the regulation of PARP1 activi…
View article: ADP‐ribosyltransferases, an update on function and nomenclature
ADP‐ribosyltransferases, an update on function and nomenclature Open
ADP‐ribosylation, a modification of proteins, nucleic acids, and metabolites, confers broad functions, including roles in stress responses elicited, for example, by DNA damage and viral infection and is involved in intra‐ and extracellular…
View article: Why structure and chain length matter: on the biological significance underlying the structural heterogeneity of poly(ADP-ribose)
Why structure and chain length matter: on the biological significance underlying the structural heterogeneity of poly(ADP-ribose) Open
Poly(ADP-ribosyl)ation (PARylation) is a multifaceted post-translational modification, carried out by poly(ADP-ribosyl)transferases (poly-ARTs, PARPs), which play essential roles in (patho-) physiology, as well as cancer therapy. Using NAD…
View article: A Multi-Endpoint Approach to Base Excision Repair Incision Activity Augmented by PARylation and DNA Damage Levels in Mice: Impact of Sex and Age
A Multi-Endpoint Approach to Base Excision Repair Incision Activity Augmented by PARylation and DNA Damage Levels in Mice: Impact of Sex and Age Open
Investigation of processes that contribute to the maintenance of genomic stability is one crucial factor in the attempt to understand mechanisms that facilitate ageing. The DNA damage response (DDR) and DNA repair mechanisms are crucial to…
View article: The Nucleolus and PARP1 in Cancer Biology
The Nucleolus and PARP1 in Cancer Biology Open
The nucleolus has been known for a long time to fulfill crucial functions in ribosome biogenesis, of which cancer cells can become addicted to in order to produce sufficient amounts of proteins for cell proliferation. Recently, the nucleol…
View article: PARP1 catalytic variants reveal branching and chain length-specific functions of poly(ADP-ribose) in cellular physiology and stress response
PARP1 catalytic variants reveal branching and chain length-specific functions of poly(ADP-ribose) in cellular physiology and stress response Open
Poly(ADP-ribosyl)ation regulates numerous cellular processes like genome maintenance and cell death, thus providing protective functions but also contributing to several pathological conditions. Poly(ADP-ribose) (PAR) molecules exhibit a r…
View article: Long-term simulation of lead concentrations in agricultural soils in relation to human adverse health effects
Long-term simulation of lead concentrations in agricultural soils in relation to human adverse health effects Open
Lead (Pb) exposure of consumers and the environment has been reduced over the past decades. Despite all measures taken, immission of Pb onto agricultural soils still occurs, with fertilizer application, lead shot from hunting activities, a…
View article: Real-time monitoring of PARP1-dependent PARylation by ATR-FTIR spectroscopy
Real-time monitoring of PARP1-dependent PARylation by ATR-FTIR spectroscopy Open
Poly-ADP-ribosylation (PARylation) is a fully reversible post-translational modification with key roles in cellular physiology. Due to the multi-domain structure of poly(ADP-ribose) polymerase-1 (PARP1) and the highly dynamic nature of the…
View article: Critical evaluation of human health risks due to hydraulic fracturing in natural gas and petroleum production
Critical evaluation of human health risks due to hydraulic fracturing in natural gas and petroleum production Open
The use of hydraulic fracturing (HF) to extract oil and natural gas has increased, along with intensive discussions on the associated risks to human health. Three technical processes should be differentiated when evaluating human health ri…
View article: Restriction of AID activity and somatic hypermutation by PARP-1
Restriction of AID activity and somatic hypermutation by PARP-1 Open
Affinity maturation of the humoral immune response depends on somatic hypermutation (SHM) of immunoglobulin (Ig) genes, which is initiated by targeted lesion introduction by activation-induced deaminase (AID), followed by error-prone DNA r…