Audrey Le Bas
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View article: Single-molecule localisation microscopy approaches reveal envelope glycoprotein clusters in single-enveloped viruses: a potential functional role?
Single-molecule localisation microscopy approaches reveal envelope glycoprotein clusters in single-enveloped viruses: a potential functional role? Open
Understanding how viruses enter and fuse with host cells is crucial for developing effective antiviral therapies. The process of viral entry and fusion involves a series of complex steps that allow the virus to breach the host cell membran…
View article: Molecular basis for the phosphorylation of bacterial tyrosine kinase Wzc
Molecular basis for the phosphorylation of bacterial tyrosine kinase Wzc Open
The regulation of polymerisation and translocation of biomolecules is fundamental. Wzc, an integral cytoplasmic membrane tyrosine autokinase protein serves as the master regulator of the biosynthesis and export of many bacterial capsular p…
View article: Structure of the Outer Membrane Transporter FemA and Its Role in the Uptake of Ferric Dihydro-Aeruginoic Acid and Ferric Aeruginoic Acid in <i>Pseudomonas aeruginosa</i>
Structure of the Outer Membrane Transporter FemA and Its Role in the Uptake of Ferric Dihydro-Aeruginoic Acid and Ferric Aeruginoic Acid in <i>Pseudomonas aeruginosa</i> Open
Iron is essential for bacterial growth, and Pseudomonas aeruginosa synthesizes the siderophores pyochelin (PCH) and pyoverdine to acquire it. PCH contains a thiazolidine ring that aids in iron chelation but is prone to hydrolysis, l…
View article: Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide
Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide Open
The enterobacterial common antigen (ECA) is conserved in Gram-negative bacteria of the Enterobacterales order although its function is debated. ECA biogenesis depends on the Wzx/Wzy-dependent strategy whereby the newly synthesized lipid-li…
View article: Structural and functional characterization of nanobodies that neutralize Omicron variants of SARS-CoV-2
Structural and functional characterization of nanobodies that neutralize Omicron variants of SARS-CoV-2 Open
The Omicron strains of SARS-CoV-2 pose a significant challenge to the development of effective antibody-based treatments as immune evasion has compromised most available immune therapeutics. Therefore, in the ‘arms race’ with the virus, th…
View article: The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex
The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex Open
The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the Enterobacteriaceae . ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of the Wzy membrane prot…
View article: Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes
Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes Open
Camelid single-domain antibodies, also known as nanobodies, can be readily isolated from naïve libraries for specific targets but often bind too weakly to their targets to be immediately useful. Laboratory-based genetic engineering methods…
View article: Pathogen-sugar interactions revealed by universal saturation transfer analysis
Pathogen-sugar interactions revealed by universal saturation transfer analysis Open
Many pathogens exploit host cell-surface glycans. However, precise analyses of glycan ligands binding with heavily modified pathogen proteins can be confounded by overlapping sugar signals and/or compounded with known experimental constrai…
View article: Correlation between the binding affinity and the conformational entropy of nanobodies targeting the SARSCoV- 2 spike protein
Correlation between the binding affinity and the conformational entropy of nanobodies targeting the SARSCoV- 2 spike protein Open
Camelid single-domain antibodies, also known as nanobodies, can be readily isolated from na.ve libraries for specific targets. However, they often bind too weakly to their targets to be immediately useful. Laboratory-based genetic engineer…
View article: Pathogen-sugar interactions revealed by universal saturation transfer analysis
Pathogen-sugar interactions revealed by universal saturation transfer analysis Open
Supporting data for the "Pathogen-sugar interactions revealed by universal saturation transfer analysis" manuscript.
View article: Pathogen-sugar interactions revealed by universal saturation transfer analysis
Pathogen-sugar interactions revealed by universal saturation transfer analysis Open
Supporting data for the "Pathogen-sugar interactions revealed by universal saturation transfer analysis" manuscript.
View article: Production and Crystallization of Nanobodies in Complex with the Receptor Binding Domain of the SARS-CoV-2 Spike Protein
Production and Crystallization of Nanobodies in Complex with the Receptor Binding Domain of the SARS-CoV-2 Spike Protein Open
The receptor binding domain (RBD) of the spike protein of SARS-CoV-2 binds angiotensin converting enzyme-2 (ACE-2) on the surface of epithelial cells, leading to fusion, and entry of the virus into the cell. This interaction can be blocked…
View article: A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19
A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19 Open
SARS-CoV-2 remains a global threat to human health particularly as escape mutants emerge. There is an unmet need for effective treatments against COVID-19 for which neutralizing single domain antibodies (nanobodies) have significant potent…
View article: A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19
A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19 Open
SARS-CoV-2 remains a global threat to human health particularly as escape mutants emerge. There is an unmet need for effective treatments against COVID-19 for which neutralizing single domain antibodies (nanobodies) have significant potent…
View article: Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 Open
View article: Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 Open
View article: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 Open
View article: Structural characterisation of a nanobody derived from a naïve library that neutralises SARS-CoV-2
Structural characterisation of a nanobody derived from a naïve library that neutralises SARS-CoV-2 Open
The SARS-CoV-2 virus is more transmissible than previous coronaviruses and causes a more serious illness than seasonal flu. The SARS-CoV-2 receptor binding domain (RBD) of the Spike protein binds to the human angiotensin-converting enzyme …
View article: Periplasmic depolymerase provides insight into ABC transporter-dependent secretion of bacterial capsular polysaccharides
Periplasmic depolymerase provides insight into ABC transporter-dependent secretion of bacterial capsular polysaccharides Open
Significance Capsules are critical virulence determinants for bacterial pathogens. They are composed of capsular polysaccharides (CPSs) with diverse structures, whose assembly on the cell surface is often powered by a conserved ABC transpo…