Explanipedia

Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex Open

Dimitri Juillot, Cyrille Billaudeau, Isabelle Mortier‐Barrière, Aurélien Barbotin, Armand Lablaine , et al. · 2025

Membrane protein ComM transiently inhibits cell division during the development of the competence state in the pathogenic bacterium Streptococcus pneumoniae , but the underlying molecular mechanisms remain unclear. Here we show that, in co…

Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria Open

Filipe Carvalho, Alexis Carreaux, Anna Sartori-Rupp, Stéphane Tachon, Anastasia D. Gazi , et al. · 2024

Quantification of membrane fluidity in bacteria using TIR-FCS Open

Aurélien Barbotin, Cyrille Billaudeau, Erdinç Sezgin, Rut Carballido‐López · 2024

Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex Open

Dimitri Juillot, Cyrille Billaudeau, Aurélien Barbotin, Armand Lablaine, Isabelle Mortier‐Barrière , et al. · 2024

Bacterial cells are known to produce inhibitors of cell division in response to stress responses and developmental programs. Knowledge of the underlying molecular mechanisms remains however largely limited. In this study, we investigated t…

Diving into bacterial dormancy: emergence of osmotically stable wall-less forms in an aquatic environment Open

Filipe Carvalho, Alexis Carreaux, Anna Sartori-Rupp, Stéphane Tachon, Anastasia D. Gazi , et al. · 2023

Bacteria can respond to environmental stresses by entering a dormant state, called viable but non-culturable (VBNC) state, in which they no longer grow in routine culture media. VBNC pathogens pose thus a significant risk for human and ani…

Quantification of membrane fluidity in bacteria using TIR-FCS Open

Aurélien Barbotin, Cyrille Billaudeau, Erdinç Sezgin, Rut Carballido‐López · 2023

Cell membrane fluidity is an important phenotypic feature that regulates the diffusion, function and folding of transmembrane and membrane-associated proteins. It is particularly interesting to study it in bacteria as variations in membran…

Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5 Open

Silvia Galiani, Katharina Reglinski, Pablo Carravilla, Aurélien Barbotin, Iztok Urbančič , et al. · 2022

Cellular functions rely on proper actions of organelles such as peroxisomes. These organelles rely on the import of proteins from the cytosol. The peroxisomal import receptor PEX5 takes up target proteins in the cytosol and transports them…

Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5 Open

Silvia Galiani, Katharina Reglinski, Pablo Carravilla, Aurélien Barbotin, Iztok Urbančič , et al. · 2021

Measuring diffusion dynamics in living cells is essential for the understanding of molecular interactions. While various techniques have been used to explore such characteristics in the plasma membrane, this is less developed for measureme…

Quantitative Methodologies to Dissect Immune Cell Mechanobiology Open

Veronika Pfannenstill, Aurélien Barbotin, Huw Colin‐York, Marco Fritzsche · 2021

Mechanobiology seeks to understand how cells integrate their biomechanics into their function and behavior. Unravelling the mechanisms underlying these mechanobiological processes is particularly important for immune cells in the context o…

Practical Implementation of Adaptive Optical Microscopes Open

Karen M. Hampson, Mantas Žurauskas, Aurélien Barbotin, Martin J. Booth · 2020

This tutorial presents guidelines that will help in the design and implementation of adaptive optics systems for microscopes.

Background Reduction in STED-FCS Using a Bivortex Phase Mask Open

Aurélien Barbotin, Iztok Urbančič, Silvia Galiani, Christian Eggeling, Martin J. Booth · 2020

Fluorescence correlation spectroscopy (FCS) is a valuable tool to study the molecular dynamics in living cells. When used together with a super-resolution stimulated emission depletion (STED) microscope, STED-FCS can measure diffusion proc…

Fast and accurate aberration estimation from 3D bead images using convolutional neural networks Open

Debayan Saha, Uwe Schmidt, Qinrong Zhang, Aurélien Barbotin, Qi Hu , et al. · 2020

Estimating optical aberrations from volumetric intensity images is a key step in sensorless adaptive optics for microscopy. Here we describe a method (PHASENET) for fast and accurate aberration measurement from experimentally acquired 3D b…

Comparison of Multiscale Imaging Methods for Brain Research Open

Jessica Tröger, Christian Hoischen, Birgit Perner, Shamci Monajembashi, Aurélien Barbotin , et al. · 2020

A major challenge in neuroscience is how to study structural alterations in the brain. Even small changes in synaptic composition could have severe outcomes for body functions. Many neuropathological diseases are attributable to disorganiz…

Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy Open

Jacopo Antonello, Aurélien Barbotin, Ee Zhuan Chong, Jens Rittscher, Martin J. Booth · 2020

Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its …

z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics Open

Aurélien Barbotin, Iztok Urbančič, Silvia Galiani, Christian Eggeling, Martin J. Booth , et al. · 2020

Super-resolution stimulated emission depletion (STED) microcopy provides optical resolution beyond the diffraction limit. The resolution can be increased laterally (xy) or axially (z). Two-dimensional STED has been extensively used to eluc…

Alignment of an SLM-based STED depletion beam Open

Aurélien Barbotin · 2020

This tutorial explains the specifics of how to set up and align the depletion beam of a STED microscope that includes a spatial light modulator (SLM).

Background reduction in STED-FCS using coherent-hybrid STED Open

Aurélien Barbotin, Iztok Urbančič, Silvia Galiani, Christian Eggeling, Martin J. Booth · 2020

Fluorescence correlation spectroscopy (FCS) is a valuable tool to study the molecular dynamics of living cells. When used together with a super-resolution stimulated emission depletion (STED) microscope, STED-FCS can measure diffusion proc…

Supplementary document for Practical sensorless aberration estimation for 3D microscopy with deep learning - 4780517.pdf Open

Martin Weigert, Uwe Schmidt, Qinrong Zhang, Aurélien Barbotin, Qi Hu , et al. · 2020

Supplemental Notes, Tables, and Figures

Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy Open

Jacopo Antonello, Aurélien Barbotin, Ee Zhuan Chong, Jens Rittscher, Martin J. Booth · 2020

z-STED imaging and spectroscopy to investigate nanoscale membrane structure and dynamics Open

Aurélien Barbotin, Iztok Urbančič, Silvia Galiani, Christian Eggeling, Martin J. Booth , et al. · 2019

Super-resolution STED microcopy provides optical resolution beyond the diffraction limit. The resolution can be increased laterally (xy/2D) or axially (z/3D). 2D STED has been extensively used to elucidate the nanoscale membrane structure …

Adaptive optics allows STED-FCS measurements in the cytoplasm of living cells Open

Aurélien Barbotin, Silvia Galiani, Iztok Urbančič, Christian Eggeling, Martin J. Booth · 2019

Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular…

Adaptive optics allows 3D STED-FCS measurements in the cytoplasm of living cells Open

Aurélien Barbotin, Silvia Galiani, Iztok Urbančič, Christian Eggeling, Martin J. Booth · 2019

Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular…

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