Explanipedia

Analysis of Factors That Regulate HIV-1 Fusion in Reverse Open

Ayna Alfadhli, Robin Lid Barklis, Fikadu Tafesse, Eric Barklis · 2025

Based on observations that HIV-1 envelope (Env) proteins on the surfaces of cells have the capacity to fuse with neighboring cells or enveloped viruses that express CD4 receptors and CXCR4 co-receptors, we tested factors that affect the ca…

ANALYSIS OF FACTORS THAT REGULATE HIV-1 FUSION IN REVERSE Open

Ayna Alfadhli, Robin Lid Barklis, Fikadu Tafesse, Eric Barklis · 2025

Based on observations that HIV-1 envelope (Env) proteins on the surfaces of cells have the capacity to fuse with neighboring cells or enveloped viruses that express CD4 receptors and CXCR4 co-receptors, we tested factors that affect the ca…

A Nanobody Interaction with SARS-CoV-2 Spike Allows the Versatile Targeting of Lentivirus Vectors Open

Ayna Alfadhli, Timothy A. Bates, Robin Lid Barklis, CeAnn Romanaggi, Fikadu Tafesse , et al. · 2024

While investigating methods to target gene delivery vectors to specific cell types, we examined the potential of using a nanobody against the SARS-CoV-2 Spike protein receptor binding domain to direct lentivirus infection of Spike-expressi…

Second site reversion of HIV-1 envelope protein baseplate mutations maps to the matrix protein Open

Ayna Alfadhli, CeAnn Romanaggi, Robin Lid Barklis, Eric Barklis · 2024

How HIV-1 Env trimers assemble into virus particles remains incompletely understood. In restrictive cells, viral incorporation of Env is dependent on the Env CT and on the MA protein, which assembles lattices composed of hexamers of trimer…

Analysis of HIV-1 envelope cytoplasmic tail effects on viral replication Open

Ayna Alfadhli, CeAnn Romanaggi, Robin Lid Barklis, Eric Barklis · 2023

Capsid-specific nanobody effects on HIV-1 assembly and infectivity Open

Ayna Alfadhli, CeAnn Romanaggi, Robin Lid Barklis, Ilaria R Merutka, Timothy A. Bates , et al. · 2021

Ceramide synthase 2 deletion decreases the infectivity of HIV-1 Open

Eric Barklis, Ayna Alfadhli, Jennifer Kyle, Lisa Bramer, Kent Bloodsworth , et al. · 2021

The lipid composition of HIV-1 virions is enriched in sphingomyelin (SM), but the roles that SM or other sphingolipids (SLs) might play in the HIV-1 replication pathway have not been elucidated. In human cells, SL levels are regulated by c…

HIV-1 Matrix Trimerization-Impaired Mutants Are Rescued by Matrix Substitutions That Enhance Envelope Glycoprotein Incorporation Open

Philip R. Tedbury, Mariia Novikova, Ayna Alfadhli, Yuta Hikichi, Ioannis Kagiampakis , et al. · 2019

The immature Gag lattice is a critical structural feature of assembling HIV-1 particles, which is primarily important for virion formation and release. While Gag forms a hexameric lattice, driven primarily by the capsid domain, the MA doma…

Replication of HIV-1 envelope protein cytoplasmic domain variants in permissive and restrictive cells Open

August O. Staubus, Ayna Alfadhli, Robin Lid Barklis, Eric Barklis · 2019

Analysis of HIV-1 Matrix-Envelope Cytoplasmic Tail Interactions Open

Ayna Alfadhli, August O. Staubus, Philip R. Tedbury, Mariia Novikova, Eric O. Freed , et al. · 2019

The mechanism by which HIV-1 envelope (Env) protein trimers assemble into virus particles is poorly understood but involves an interaction between Env cytoplasmic tails (CTs) and the matrix (MA) domain of the structural precursor Gag (PrGa…

Organization of Farnesylated, Carboxymethylated KRAS4B on Membranes Open

Eric Barklis, Andrew G. Stephen, August O. Staubus, Robin Lid Barklis, Ayna Alfadhli · 2019

Lipid biosensor interactions with wild type and matrix deletion HIV-1 Gag proteins Open

Eric Barklis, August O. Staubus, Andrew R. Mack, Logan Harper, Robin Lid Barklis , et al. · 2018

Analysis of K-Ras Interactions by Biotin Ligase Tagging Open

Christopher Ritchie, Andrew R. Mack, Logan Harper, Ayna Alfadhli, Philip A. Stork , et al. · 2017

Biotin ligase tagging offers a rapid and convenient approach to the characterization of K-Ras interaction networks.

Analysis of quinolinequinone reactivity, cytotoxicity, and anti-HIV-1 properties Open

Ayna Alfadhli, Andrew R. Mack, Logan Harper, Sam Berk, Christopher Ritchie , et al. · 2016

Trimer Enhancement Mutation Effects on HIV-1 Matrix Protein Binding Activities Open

Ayna Alfadhli, Andrew R. Mack, Christopher Ritchie, Isabel Cylinder, Logan Harper , et al. · 2016

The HIV-1 matrix (MA) protein is the amino-terminal domain of the HIV-1 precursor Gag (Pr55Gag) protein. MA binds to membranes and RNAs, helps transport Pr55Gag proteins to virus assembly sites at the plasma membranes of infected cells, an…

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