Benoit G. Bruneau
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View article: A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects
A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects Open
Failure of septation of the interventricular septum (IVS) is the most common congenital heart defect, but mechanisms for patterning the IVS are largely unknown. Here we show that a Tbx5 + /Mef2cAHF + progenitor lineage forms a compartment …
View article: MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesis
MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesis Open
The gene regulatory networks (GRNs) that control early heart formation are beginning to be understood, but lineage-specific GRNs remain largely undefined. We investigated networks controlled by the vital transcription factor MEF2C using a …
View article: Reduced TBX5 dosage undermines developmental control of atrial cardiomyocyte identity in a model of human atrial disease
Reduced TBX5 dosage undermines developmental control of atrial cardiomyocyte identity in a model of human atrial disease Open
While atrial septal defects (ASDs) and atrial fibrillation (AF) present differently, there is evidence that they share some genetic basis. Here, we used directed differentiation of human induced pluripotent stem cells into atrial or ventri…
View article: The Interplay of Ontogeny and Phylogeny at the Transcriptome Level of the Tetrapod Heart
The Interplay of Ontogeny and Phylogeny at the Transcriptome Level of the Tetrapod Heart Open
The tetrapod heart is characterized by three chambers in amphibians and non-avian reptiles, as opposed to four in birds, crocodilians and mammals. We explored this diversity via the most phylogenetically comprehensive comparison of heart t…
View article: The Interplay of Ontogeny and Phylogeny at the Transcriptome Level of the Tetrapod Heart
The Interplay of Ontogeny and Phylogeny at the Transcriptome Level of the Tetrapod Heart Open
The tetrapod heart is characterized by three chambers in amphibians and non‐avian reptiles, as opposed to four in birds, crocodilians and mammals. We explored this diversity via the most phylogenetically comprehensive comparison of heart t…
View article: Modeling <i>SMAD2</i> Mutations in Induced Pluripotent Stem Cells Provides Insights Into Cardiovascular Disease Pathogenesis
Modeling <i>SMAD2</i> Mutations in Induced Pluripotent Stem Cells Provides Insights Into Cardiovascular Disease Pathogenesis Open
Background SMAD2 is a coregulator that binds a variety of transcription factors in human development. Heterozygous SMAD2 loss‐of‐function and missense variants are identified in patients with congenital heart disease (CHD) or arterial aneu…
View article: Dose-dependent sensitivity of human 3D chromatin to a heart disease-linked transcription factor
Dose-dependent sensitivity of human 3D chromatin to a heart disease-linked transcription factor Open
Dosage-sensitive transcription factors (TFs) underlie altered gene regulation in human developmental disorders, and cell-type specific gene regulation is linked to the reorganization of 3D chromatin during cellular differentiation. Here, w…
View article: 4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos
4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos Open
As light sheet fluorescence microscopy (LSFM) becomes widely available, reconstruction of time-lapse imaging will further our understanding of complex biological processes at cellular resolution. Here, we present a comprehensive workflow f…
View article: MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesis
MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesis Open
The gene regulatory networks (GRNs) that control early heart formation are beginning to be understood, but lineage-specific GRNs remain largely undefined. We investigated networks controlled by the vital transcription factor MEF2C, with a …
View article: CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development
CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development Open
Loss of Cdx2 in vivo leads to stunted development of the allantois, an extraembryonic mesoderm-derived structure critical for nutrient delivery and waste removal in the early embryo. Here, we investigate how CDX2 dose-dependently influence…
View article: First person – Emily Bulger
First person – Emily Bulger Open
First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping researchers promote themselves alongside their papers. Emily Bulger is first author on ‘ CDX2 dose-dependently influe…
View article: A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects
A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects Open
Failure of septation of the interventricular septum (IVS) is the most common congenital heart defect (CHD), but mechanisms for patterning the IVS are largely unknown. Here, we show that a Tbx5 + /Mef2cAHF + progenitor lineage forms a compa…
View article: CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development
CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development Open
Proper regulation of gene dosage is critical for the development of the early embryo and the extraembryonic tissues that support it. Specifically, loss of Cdx2 in vivo leads to stunted development of the allantois, an extraembryonic mesode…
View article: TBXT dose sensitivity and the decoupling of nascent mesoderm specification from EMT progression in 2D human gastruloids
TBXT dose sensitivity and the decoupling of nascent mesoderm specification from EMT progression in 2D human gastruloids Open
In the nascent mesoderm, levels of Brachyury (TBXT) expression must be precisely regulated to ensure cells exit the primitive streak and pattern the anterior-posterior axis, but how this varying dosage informs morphogenesis is not well und…
View article: A <i>Mesp1</i> -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
A <i>Mesp1</i> -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors Open
Transcriptional networks governing cardiac precursor cell (CPC) specification are incompletely understood owing, in part, to limitations in distinguishing CPCs from non-cardiac mesoderm in early gastrulation. We leveraged detection of earl…
View article: Sanger sequencing data to verify mouse Dppa locus deletion
Sanger sequencing data to verify mouse Dppa locus deletion Open
Identifying cis Elements for Spatiotemporal Control of Mammalian DNA Replication.Sima J, Chakraborty A, Dileep V, Michalski M, Klein KN, Holcomb NP, Turner JL, Paulsen MT, Rivera-Mulia JC, Trevilla-Garcia C, Bartlett DA, Zhao PA, Washburn …
View article: Sanger sequencing data to verify mouse Rex1 locus deletion
Sanger sequencing data to verify mouse Rex1 locus deletion Open
Identifying cis Elements for Spatiotemporal Control of Mammalian DNA Replication.Sima J, Chakraborty A, Dileep V, Michalski M, Klein KN, Holcomb NP, Turner JL, Paulsen MT, Rivera-Mulia JC, Trevilla-Garcia C, Bartlett DA, Zhao PA, Washburn …
View article: A <i>Mesp1</i>-dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
A <i>Mesp1</i>-dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors Open
Transcriptional networks governing cardiac precursor cell (CPC) specification are incompletely understood due in part to limitations in distinguishing CPCs from non-cardiac mesoderm in early gastrulation. We leveraged detection of early ca…
View article: Decision letter: An atrial fibrillation-associated regulatory region modulates cardiac Tbx5 levels and arrhythmia susceptibility
Decision letter: An atrial fibrillation-associated regulatory region modulates cardiac Tbx5 levels and arrhythmia susceptibility Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Heart development and …
View article: Transcription Factor GATA4 Regulates Cell Type–Specific Splicing Through Direct Interaction With RNA in Human Induced Pluripotent Stem Cell–Derived Cardiac Progenitors
Transcription Factor GATA4 Regulates Cell Type–Specific Splicing Through Direct Interaction With RNA in Human Induced Pluripotent Stem Cell–Derived Cardiac Progenitors Open
Background: GATA4 (GATA-binding protein 4), a zinc finger–containing, DNA-binding transcription factor, is essential for normal cardiac development and homeostasis in mice and humans, and mutations in this gene have been reported in human …
View article: A spatiotemporal gradient of mesoderm assembly governs cell fate and morphogenesis of the early mammalian heart
A spatiotemporal gradient of mesoderm assembly governs cell fate and morphogenesis of the early mammalian heart Open
Using four-dimensional whole-embryo light sheet imaging with improved and accessible computational tools, we longitudinally reconstruct early murine cardiac development at single-cell resolution. Nascent mesoderm progenitors form opposing …