Bryan R. Cullen
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View article: Efficacious genome editing in infant mice with glycogen storage disease type Ia
Efficacious genome editing in infant mice with glycogen storage disease type Ia Open
Glycogen storage disease type Ia (GSD Ia) is caused by a deficiency of glucose-6-phosphatase (G6Pase) in the liver leading to lethal hypoglycemia. Gene therapy with adeno-associated virus (AAV) vectors encoding G6Pase fails to stably treat…
View article: Differential sensitivity of murine leukemia virus to APOBEC3-mediated inhibition is governed by virion exclusion
Differential sensitivity of murine leukemia virus to APOBEC3-mediated inhibition is governed by virion exclusion Open
While members of the APOBEC3 family of human intrinsic resistance factors are able to restrict the replication of Vif-deflcient forms of human immunodeficiency virus type 1 (HIV-1), they are unable to block replication of wild-type HIV-1 d…
View article: Foamy virus Bet proteins function as novel inhibitors of the APOBEC3 family of innate antiretroviral defense factors
Foamy virus Bet proteins function as novel inhibitors of the APOBEC3 family of innate antiretroviral defense factors Open
Foamy viruses are a family of complex retroviruses that establish common, productive infections in a wide range of nonhuman primates. In contrast, humans appear nonpermissive for foamy virus replication, although zoonotic infections do occ…
View article: Inhibition of a yeast LTR retrotransposon by human APOBEC3 cytidine deaminases
Inhibition of a yeast LTR retrotransposon by human APOBEC3 cytidine deaminases Open
The mammalian APOBEC3 family of cytidine deaminases includes several members that possess potent antiretroviral activity. Human APOBEC3F and APOBEC3G are specifically incorporated into human immunodeficiency virus type 1 (HIV-1) progeny vi…
View article: Cloning and analysis of microRNAs encoded by the primate γ-herpesvirus rhesus monkey rhadinovirus
Cloning and analysis of microRNAs encoded by the primate γ-herpesvirus rhesus monkey rhadinovirus Open
Several pathogenic human herpesviruses have recently been shown to express virally encoded microRNAs in infected cells. Although the function of these microRNAs is largely unknown, they are hypothesized to play a role in mediating viral re…
View article: Genome editing using Staphylococcus aureus Cas9 in a canine model of glycogen storage disease Ia
Genome editing using Staphylococcus aureus Cas9 in a canine model of glycogen storage disease Ia Open
Glycogen storage disease type Ia (GSD Ia) is the inherited deficiency of glucose-6-phosphatase (G6Pase), associated with life-threatening hypoglycemia and long-term complications, including hepatocellular carcinoma formation. Gene replacem…
View article: RNA conformational propensities determine cellular activity
RNA conformational propensities determine cellular activity Open
Cellular processes are the product of interactions between biomolecules, which associate to form biologically active complexes 1 . These interactions are mediated by intermolecular contacts, which if disrupted, lead to alterations in cell …
View article: Epigenetic silencing by the SMC5/6 complex mediates HIV-1 latency
Epigenetic silencing by the SMC5/6 complex mediates HIV-1 latency Open
After viral entry and reverse transcription, HIV-1 proviruses that fail to integrate are epigenetically silenced, but the underlying mechanism has remained unclear. Using a genome-wide CRISPR/Cas9 knockout screen, we identified the host SM…
View article: Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique
Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique Open
Pseudouridine (Ψ) is the most common noncanonical ribonucleoside present on mammalian noncoding RNAs (ncRNAs), including rRNAs, tRNAs, and snRNAs, where it contributes ∼7% of the total uridine level. However, Ψ constitutes only ∼0.1% of th…
View article: Epitranscriptomic addition of m <sup>6</sup> A regulates HIV-1 RNA stability and alternative splicing
Epitranscriptomic addition of m <sup>6</sup> A regulates HIV-1 RNA stability and alternative splicing Open
Previous work has demonstrated that the epitranscriptomic addition of m 6 A to viral transcripts can promote the replication and pathogenicity of a wide range of DNA and RNA viruses, including HIV-1, yet the underlying mechanisms responsib…
View article: Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique
Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique Open
Pseudouridine (Ψ) is the most common non-canonical ribonucleoside present on mammalian non-coding RNAs (ncRNAs), including rRNAs, tRNAs and snRNAs, where it contributes ∼7% of the total uridine level. However, Ψ constitutes only ∼0.1% of t…
View article: Epitranscriptomic addition of m<sup>6</sup>A regulates HIV-1 RNA stability and alternative splicing
Epitranscriptomic addition of m<sup>6</sup>A regulates HIV-1 RNA stability and alternative splicing Open
Previous work in several laboratories has demonstrated that the epitranscriptomic addition of m 6 A to viral transcripts promotes the replication and pathogenicity of a wide range of DNA and RNA viruses, yet the underlying mechanisms respo…
View article: Tax induces the recruitment of NF-kB to unintegrated HIV-1 DNA to rescue viral gene expression and replication
Tax induces the recruitment of NF-kB to unintegrated HIV-1 DNA to rescue viral gene expression and replication Open
We have previously reported that the normally essential step of integration of the HIV-1 proviral DNA intermediate into the host cell genome becomes dispensable in T cells that express the Human T cell leukemia virus 1 (HTLV-1) Tax protein…
View article: Epstein–Barr Virus MicroRNAs Are Evolutionarily Conserved and Differentially Expressed
Epstein–Barr Virus MicroRNAs Are Evolutionarily Conserved and Differentially Expressed Open
The pathogenic lymphocryptovirus Epstein–Barr virus (EBV) is shown to express at least 17 distinct microRNAs (miRNAs) in latently infected cells. These are arranged in two clusters: 14 miRNAs are located in the introns of the viral BART ge…
View article: Understanding the characteristics of nonspecific binding of drug-like compounds to canonical stem–loop RNAs and their implications for functional cellular assays
Understanding the characteristics of nonspecific binding of drug-like compounds to canonical stem–loop RNAs and their implications for functional cellular assays Open
Identifying small molecules that selectively bind an RNA target while discriminating against all other cellular RNAs is an important challenge in RNA-targeted drug discovery. Much effort has been directed toward identifying drug-like small…
View article: Reversal of Epigenetic Silencing Allows Robust HIV-1 Replication in the Absence of Integrase Function
Reversal of Epigenetic Silencing Allows Robust HIV-1 Replication in the Absence of Integrase Function Open
While retroviral DNA is synthesized normally after infection by integrase-deficient viruses, the resultant episomal DNA is then epigenetically silenced. Here, we show that expression of the Tax transcription factor encoded by a second huma…
View article: Revealing the high propensity of RNAs to non-specifically bind drug-like small molecules
Revealing the high propensity of RNAs to non-specifically bind drug-like small molecules Open
Identifying small molecules that selectively bind a single RNA target while discriminating against all other cellular RNAs is an important challenge in RNA-targeted drug discovery. Much effort has been directed toward identifying drug-like…
View article: Robust HIV-1 replication in the absence of integrase function
Robust HIV-1 replication in the absence of integrase function Open
Integration of the proviral DNA intermediate into the host cell genome represents an essential step in the retroviral life cycle. While the reason(s) for this requirement remains unclear, it is known that unintegrated proviral DNA is epige…
View article: Acetylation of cytidine residues boosts HIV-1 gene expression by increasing viral RNA stability
Acetylation of cytidine residues boosts HIV-1 gene expression by increasing viral RNA stability Open
Covalent modifications added to individual nucleotides on mRNAs, called epitranscriptomic modifications, have recently emerged as key regulators of both cellular and viral mRNA function 1,2 and RNA methylation has now been shown to enhance…
View article: Decision letter: Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9
Decision letter: Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9 Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Herpes simplex virus (HSV) establishes lif…