Explanipedia

Machine learning approaches for the prediction of public EV charge point flexibility Open

Federica Bellizio, Bauke W. Dijkstra, Angelika Fertig, Jules Van Dijk, Philipp Heer · 2025

The increasing number of electric vehicles on the road can play a key role as a source of flexibility for a reliable power system operation. Charge point operators, in particular, can adjust individual electric vehicle charging loads to pr…

Machine Learning Approaches for the Prediction of Public Ev Charge Point Flexibility Open

Federica Bellizio, Bauke W. Dijkstra, Angelika Fertig, Jules Van Dijk, Philipp Heer · 2024

Structural and time‐resolved mechanistic investigations of protein hydrolysis by the acidic proline‐specific endoprotease from Aspergillus niger Open

Tjaard Pijning, Andreja Vujičić‐Žagar, Jan‐Metske van der Laan, René M. de Jong, Carlos Ramírez-Palacios , et al. · 2023

Proline‐specific endoproteases have been successfully used in, for example, the in‐situ degradation of gluten, the hydrolysis of bitter peptides, the reduction of haze during beer production, and the generation of peptides for mass spectro…

The Role of Tryptophan in π Interactions in Proteins: An Experimental Approach Open

Jinfeng Shao, Bastiaan P. Kuiper, A.M.W.H. Thunnissen, Robbert H. Cool, Liang Zhou , et al. · 2022

In proteins, the amino acids Phe, Tyr, and especially Trp are frequently involved in π interactions such as π-π, cation-π, and CH-π bonds. These interactions are often crucial for protein structure and protein-ligand binding. A powerful me…

Application of microbial 3-ketosteroid Δ1-dehydrogenases in biotechnology Open

Ali Rohman, Bauke W. Dijkstra · 2021

Review for "Structures of the TetR ‐like transcription regulator RcdA alone and in complexes with ligands" Open

Bauke W. Dijkstra · 2021

Phenylthiourea Binding to Human Tyrosinase-Related Protein 1 Open

Xuelei Lai, Harry J. Wichers, Montserrat Soler‐López, Bauke W. Dijkstra · 2020

Tyrosinase-related protein 1 (TYRP1) is one of the three human melanogenic enzymes involved in the biosynthesis of melanin, a pigment responsible for the color of the skin, hair, and eyes. It shares high sequence identity with tyrosinase, …

Xylanases from marine microorganisms: A brief overview on scope, sources, features and potential applications Open

Fatemeh Izadpanah Qeshmi, Ahmad Homaei, Pedro Fernandes, Roohullah Hemmati, Bauke W. Dijkstra , et al. · 2019

β-Xylosidases: Structural Diversity, Catalytic Mechanism, and Inhibition by Monosaccharides Open

Ali Rohman, Bauke W. Dijkstra, Ni Nyoman Tri Puspaningsih · 2019

Xylan, a prominent component of cellulosic biomass, has a high potential for degradation into reducing sugars, and subsequent conversion into bioethanol. This process requires a range of xylanolytic enzymes. Among them, β-xylosidases are c…

The role and mechanism of microbial 3-ketosteroid Δ1-dehydrogenases in steroid breakdown Open

Ali Rohman, Bauke W. Dijkstra · 2019

3-Ketosteroid Δ¹-dehydrogenases are FAD-dependent enzymes that catalyze the introduction of a double bond between the C1 and C2 atoms of the A-ring of 3-ketosteroid substrates. These enzymes are found in a large variety of micro…

Structural basis of product inhibition by arabinose and xylose of the thermostable GH43 β-1,4-xylosidase from Geobacillus thermoleovorans IT-08 Open

Ali Rohman, N. van Oosterwijk, Ni Nyoman Tri Puspaningsih, Bauke W. Dijkstra · 2018

Complete degradation of the xylan backbone of hemicellulosic plant cell walls requires the synergistic action of endo-xylanases and β-1,4-xylosidases. While endo-xylanases produce xylooligosaccharides from xylan, β-1,4-xylosidases degrade …

Frontispiece: Structure and Function of Human Tyrosinase and Tyrosinase‐Related Proteins Open

Xuelei Lai, Harry J. Wichers, Montserrat Soler‐López, Bauke W. Dijkstra · 2018

Melanin is key for the pigmentation of skin, hair and eyes in mammals. Tyrosinases are melanogenic metalloenzymes whose molecular mechanisms are largely unknown. Difficulties with producing these enzymes in pure form have hampered the unde…

Mechanism of allostery and catalysis in P. falciparum GMP Synthetase Open

Santosh Shivakumaraswamy, Lionel Ballut, Sébastien Violot, Lakshmi Prasoona Thota, Bauke W. Dijkstra , et al. · 2017

International audience

Structure and Function of Human Tyrosinase and Tyrosinase‐Related Proteins Open

Xuelei Lai, Harry J. Wichers, Montserrat Soler‐López, Bauke W. Dijkstra · 2017

Melanin is the main pigment responsible for the color of human skin, hair and eye. Its biosynthesis requires three melanogenic enzymes, tyrosinase (TYR), and the tyrosinase‐related proteins TYRP1 and TYRP2. The difficulty of isolating pure…

Study of response of Swiss Webster mice to light subunit of mushroom tyrosinase Open

Wangsa T. Ismaya, Alida Efthyani, DS Retnoningrum, Xuelei Lai, Bauke W. Dijkstra , et al. · 2017

The light subunit of mushroom, Agaricus bisporus, tyrosinase (LSMT), has been identified as an extrinsic component of the enzyme. Its function is unknown, but it can cross an epithelial cell layer, which suggests that it can be absorbed by…

Structure of Human Tyrosinase Related Protein 1 Reveals a Binuclear Zinc Active Site Important for Melanogenesis Open

Xuelei Lai, Harry J. Wichers, Montserrat Soler‐López, Bauke W. Dijkstra · 2017

Tyrosinase‐related protein 1 (TYRP1) is one of three tyrosinase‐like glycoenzymes in human melanocytes that are key to the production of melanin, the compound responsible for the pigmentation of skin, eye, and hair. Difficulties with produ…

Structure of Human Tyrosinase Related Protein 1 Reveals a Binuclear Zinc Active Site Important for Melanogenesis Open

Xuelei Lai, Harry J. Wichers, Montserrat Soler‐López, Bauke W. Dijkstra · 2017

Tyrosinase‐related protein 1 (TYRP1) is one of three tyrosinase‐like glycoenzymes in human melanocytes that are key to the production of melanin, the compound responsible for the pigmentation of skin, eye, and hair. Difficulties with produ…

The light subunit of mushroom Agaricus bisporus tyrosinase: Its biological characteristics and implications Open

Wangsa T. Ismaya, Olivia Mayasari Tandrasasmita, Syaripah Sundari, Diana Diana, Xuelei Lai , et al. · 2017

Crystal Structure of 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria Open

Yuxiang Bai, Joana Gangoiti, Bauke W. Dijkstra, Lubbert Dijkhuizen, Tjaard Pijning · 2017

Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies Open

Xuelei Lai, Montserrat Soler‐López, Harry J. Wichers, Bauke W. Dijkstra · 2016

Human tyrosinase (TYR) is a glycoprotein that initiates the first two reactions in the melanin biosynthesis pathway. Mutations in its encoding gene cause Oculocutaneous Albinism type I (OCA1), the most severe form of albinism, which is a g…

Structural Basis of the Substrate Range and Enantioselectivity of Two (S)-Selective ω-Transaminases Open

N. van Oosterwijk, Simon C. Willies, Johan Hekelaar, Anke C. Terwisscha van Scheltinga, Nicholas J. Turner , et al. · 2016

ω-Transaminases are enzymes that can introduce an amino group in industrially interesting compounds. We determined crystal structures of two (S)-selective ω-transaminases, one from Arthrobacter sp. (Ars-ωTA) and one from Bacillus megateriu…

A novel immune-tolerable and permeable lectin-like protein from mushroom Agaricus bisporus Open

Wangsa T. Ismaya, Yunita, Alida Efthyani, Xuelei Lai, Debbie S. Retnoningrum , et al. · 2016

Crystal structure of recombinant tyrosinase-binding protein MtaL at 1.35 Å resolution Open

Xuelei Lai, Montserrat Soler‐López, Wangsa T. Ismaya, Harry J. Wichers, Bauke W. Dijkstra · 2016

Mushroom tyrosinase-associated lectin-like protein (MtaL) binds to mature Agaricus bisporus tyrosinase in vivo , but the exact physiological function of MtaL is unknown. In this study, the crystal structure of recombinant MtaL is reported …

Structure and Catalytic Mechanism of 3-Ketosteroid Dehydrogenases Open

Bauke W. Dijkstra, N. van Oosterwijk, Ali Rohman · 2016

3-Ketosteroid dehydrogenases (KSTDs) are FAD-dependent enzymes that introduce a double bond in the A ring of 3-ketosteroid substrates to initiate degradation of the steroid nucleus. Δ1-KSTD desaturates the C1-C2 bond of the steroid, while …

Mutation Analysis of the pKa Modulator Residue in β-D-xylosidase from Geobacillus Thermoleovorans IT-08: Activity Adaptation to Alkaline and High-Temperature Conditions Open

Lanny Hartanti, Ali Rohman, A.C. Suwandi, Bauke W. Dijkstra, Zeily Nurahman , et al. · 2016

β-D-xylosidases are hemicellulases that catalyze the release of xylose units from short xylooligosaccharides. Their activity is the rate-limiting step in xylan hydrolysis. A major application of these enzymes is as eco-friendly biobleachin…

Active site coupling in Plasmodium falciparum GMP synthetase is triggered by domain rotation Open

Lionel Ballut, Sébastien Violot, Santosh Shivakumaraswamy, Lakshmi Prasoona Thota, M Sathya , et al. · 2015

Crystal structure of quinone‐dependent alcohol dehydrogenase from Pseudogluconobacter saccharoketogenes. A versatile dehydrogenase oxidizing alcohols and carbohydrates Open

H.J. Rozeboom, Shukun Yu, René Mikkelsen, Igor Nikolaev, Harm J. Mulder , et al. · 2015

The quinone‐dependent alcohol dehydrogenase (PQQ‐ADH, E.C. 1.1.5.2) from the Gram‐negative bacterium Pseudogluconobacter saccharoketogenes IFO 14464 oxidizes primary alcohols (e.g. ethanol, butanol), secondary alcohols (monosaccharides), a…

Structure and mechanism studies of human tyrosinase Open

Xuelei Lai, Montserrat Soler‐López, Harry J. Wichers, Bauke W. Dijkstra · 2015

X-ray crystallographic validation of structure predictions used in computational design for protein stabilization Open

Robert J. Floor, Hein J. Wijma, Peter A. Jekel, Anke C. Terwisscha van Scheltinga, Bauke W. Dijkstra , et al. · 2015

Protein engineering aimed at enhancing enzyme stability is increasingly supported by computational methods for calculation of mutant folding energies and for the design of disulfide bonds. To examine the accuracy of mutant structure predic…

Bauke W. Dijkstra YOU? Author Swipe