Bernd Hoffmann
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View article: Two proteins, one goal: ELISAs based on p32 and L1R for LSDV antibodies detection
Two proteins, one goal: ELISAs based on p32 and L1R for LSDV antibodies detection Open
Introduction Lumpy skin disease virus (LSDV), a member of the Capripoxvirus genus, poses a significant threat to livestock health and productivity in both endemic and newly affected regions. The disease is primarily transmitted by blood-fe…
View article: Development of Quantitative Real-Time PCR Tests for the Identification of Biting Midge Species and Clades (Diptera: Ceratopogonidae) of the Obsoletus Group (Subgenus Avaritia), Including Important Viral Vectors in Europe
Development of Quantitative Real-Time PCR Tests for the Identification of Biting Midge Species and Clades (Diptera: Ceratopogonidae) of the Obsoletus Group (Subgenus Avaritia), Including Important Viral Vectors in Europe Open
Species of the widespread Obsoletus Complex (Culicoides subgenus Avarita Fox, 1955) have been implicated as potential key vectors during the bluetongue and Schmallenberg epidemics in Central Europe in 2006 and 2012. Although extensive effo…
View article: Development of New Probe-Based Real-Time RT-qPCR Assays for the Detection of All Known Strains of Bovine Ephemeral Fever Viruses
Development of New Probe-Based Real-Time RT-qPCR Assays for the Detection of All Known Strains of Bovine Ephemeral Fever Viruses Open
Bovine ephemeral fever is an arthropod-borne viral disease that affects cattle and buffalo in many regions of the world; it causes heavy economic losses in the cattle industry. To date, all BEFV-specific diagnostic molecular assays have be…
View article: Experimental BTV-3 and BTV-8 infection of<i>Culicoides sonorensis</i>biting midges
Experimental BTV-3 and BTV-8 infection of<i>Culicoides sonorensis</i>biting midges Open
BTV-3 emerged in the Netherlands in 2023 and spread rapidly to neighboring countries. Compared to the BTV-8 outbreak in 2006, the course of the BTV-3 epizootic is more severe. Experimental infection of laboratory-reared Culicoides sonorens…
View article: Monitoring of Astroviruses, Brno-Hantaviruses, Coronaviruses, Influenza Viruses, Bornaviruses, Morbilliviruses, Lyssaviruses and Pestiviruses in Austrian Bats
Monitoring of Astroviruses, Brno-Hantaviruses, Coronaviruses, Influenza Viruses, Bornaviruses, Morbilliviruses, Lyssaviruses and Pestiviruses in Austrian Bats Open
Here, we report the results of a monitoring study of bat viruses in Austria to strengthen the knowledge of circulating viruses in Austrian bat populations. In this study, we analyzed 618 oropharyngeal and rectal swab samples from 309 bats …
View article: Bovine Ephemeral Fever Viruses in Israel 2014–2023: Genetic Characterization of Local and Emerging Strains
Bovine Ephemeral Fever Viruses in Israel 2014–2023: Genetic Characterization of Local and Emerging Strains Open
Bovine ephemeral fever (BEF) is an arthropod-borne viral disease, which frequently causes significant epizootics in susceptible water buffalo and cattle in Africa, Australia, Asia and the Middle East. In the current study, a two-stage prot…
View article: Prevalence of tick-borne bacterial pathogens in Germany—has the situation changed after a decade?
Prevalence of tick-borne bacterial pathogens in Germany—has the situation changed after a decade? Open
Introduction Tick-borne pathogens, such as Borreliella spp., Rickettsia spp., and Anaplasma spp., are frequently detected in Germany. They circulate between animals and tick vectors and can cause mild to severe diseases in humans. Knowledg…
View article: Bluetongue Virus Serotype 3 and Schmallenberg Virus in <i>Culicoides</i> Biting Midges, Western Germany, 2023
Bluetongue Virus Serotype 3 and Schmallenberg Virus in <i>Culicoides</i> Biting Midges, Western Germany, 2023 Open
In October 2023, bluetongue virus serotype 3 (BTV-3) emerged in Germany, where Schmallenberg virus is enzootic. We detected BTV-3 in 1 pool of Culicoides biting midges collected at the time ruminant infections were reported. Schmallenberg …
View article: Emergence of bluetongue virus serotype 3 in western Germany, October 2023, and<i>ad-hoc</i>monitoring in<i>Culicoides</i>biting midges
Emergence of bluetongue virus serotype 3 in western Germany, October 2023, and<i>ad-hoc</i>monitoring in<i>Culicoides</i>biting midges Open
Bluetongue virus serotype 3 emerged in October 2023 in Germany, where Schmallenberg virus is enzootic. BTV-3 was detected in a pool of Culicoides biting midges at the same time as infections were reported from ruminants. Schmallenberg viru…
View article: The Molecular Epidemiology of Epizootic Hemorrhagic Disease Viruses Identified in Israel between 2015 and 2023
The Molecular Epidemiology of Epizootic Hemorrhagic Disease Viruses Identified in Israel between 2015 and 2023 Open
Epizootic hemorrhagic disease (EHD) is an infectious, non-contagious viral disease seriously affecting cattle and some wild ruminants and has a worldwide distribution. All viruses can be subdivided into “Eastern” and “Western” topotypes ac…
View article: Lethal Borna disease virus 1 (BoDV-1) infections of humans and animals – in-depth molecular epidemiology and phylogeography
Lethal Borna disease virus 1 (BoDV-1) infections of humans and animals – in-depth molecular epidemiology and phylogeography Open
Borna disease virus 1 (BoDV-1) is the causative agent of Borna disease, a progressive and mostly fatal neurologic disorder of domestic mammals and humans, resulting from spill-over infection from its natural reservoir host, the bicolored w…
View article: Exploring a new machine learning based probabilistic model for high-resolution indoor radon mapping, using the German indoor radon survey data
Exploring a new machine learning based probabilistic model for high-resolution indoor radon mapping, using the German indoor radon survey data Open
Radon is a carcinogenic, radioactive gas that can accumulate indoors. Therefore, accurate knowledge of indoor radon concentration is crucial for assessing radon-related health effects or identifying radon-prone areas. Indoor radon concentr…
View article: ELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease
ELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease Open
Lumpy skin disease (LSD) is a notifiable, transboundary cattle disease that spreads rapidly and has a relevant economic impact. The etiological agent is the LSD virus (LSDV), genus Capripoxvirus, and family Poxviridae. To date, LSDV is wid…
View article: Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response
Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response Open
The peste des petits ruminant (PPR) virus is a transboundary virus found in small domestic ruminants that causes high morbidity and mortality in naive herds. PPR can be effectively controlled and eradicated by vaccinating small domestic ru…
View article: Cross-Protection of an Inactivated and a Live-Attenuated Lumpy Skin Disease Virus Vaccine against Sheeppox Virus Infections in Sheep
Cross-Protection of an Inactivated and a Live-Attenuated Lumpy Skin Disease Virus Vaccine against Sheeppox Virus Infections in Sheep Open
Sheeppox virus (SPPV) (genus Capripoxvirus, family Poxviridae) infections are a highly virulent and contagious disease of sheep with a high morbidity and mortality, especially in naïve populations and young animals. For the control of SPPV…
View article: Estimating national indoor radon exposure at a high spatial resolution – improvements by a machine learning based probabilistic approach
Estimating national indoor radon exposure at a high spatial resolution – improvements by a machine learning based probabilistic approach Open
Accurate knowledge of indoor radon exposure is vital information for assessing radon-induced health effects, identifying radon prone areas or estimating the number of people affected by the exceedance of a specific radon concentration in a…
View article: Comparative Molecular and Epidemiological Analyses of Israeli Bluetongue Viruses Serotype 1 and 9 Causing Outbreaks in 2018–2020
Comparative Molecular and Epidemiological Analyses of Israeli Bluetongue Viruses Serotype 1 and 9 Causing Outbreaks in 2018–2020 Open
Israel is endemic to bluetongue virus (BTV). The introduction of novel-for-the-region arboviruses have been recorded annually in recent years. In 2019, previously non-reported in-the-country BTV-1 and BTV-9 were identified. BTV-1 caused a …
View article: Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe Open
Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats ( Nyctalus noctula ) in the Czech Republic, but nothing is known about its…
View article: Point-of-Care Testing for Sensitive Detection of the African Swine Fever Virus Genome
Point-of-Care Testing for Sensitive Detection of the African Swine Fever Virus Genome Open
African swine fever (ASF) is a contagious viral hemorrhagic disease that affects domestic pigs and wild boar. The disease is notifiable to the World Organization of Animal Health (WOAH), and causes significant deaths and economic losses. T…
View article: Clinical Epidemiology, Pathology, and Molecular Investigation of Lumpy Skin Disease Outbreaks in Bangladesh during 2020–2021 Indicate the Re-Emergence of an Old African Strain
Clinical Epidemiology, Pathology, and Molecular Investigation of Lumpy Skin Disease Outbreaks in Bangladesh during 2020–2021 Indicate the Re-Emergence of an Old African Strain Open
Lumpy skin disease (LSD) emerged in Bangladesh in mid-2019, leading to great economic losses for cattle farmers. This study describes the recent occurrence of the LSDV in Bangladesh and examines the clinical manifestation of the disease in…
View article: Comparison of Biosafety and Diagnostic Utility of Biosample Collection Cards
Comparison of Biosafety and Diagnostic Utility of Biosample Collection Cards Open
Six different biosample collection cards, often collectively referred to as FTA (Flinders Technology Associates) cards, were compared for their ability to inactivate viruses and stabilize viral nucleic acid for molecular testing. The cards…
View article: The spike gene is a major determinant for the SARS-CoV-2 Omicron-BA.1 phenotype
The spike gene is a major determinant for the SARS-CoV-2 Omicron-BA.1 phenotype Open
Variant of concern (VOC) Omicron-BA.1 has achieved global predominance in early 2022. Therefore, surveillance and comprehensive characterization of Omicron-BA.1 in advanced primary cell culture systems and animal models are urgently needed…
View article: Comparison of Extraction Methods for the Detection of Tick-Borne Encephalitis Virus RNA in Goat Raw Milk and Cream Cheese
Comparison of Extraction Methods for the Detection of Tick-Borne Encephalitis Virus RNA in Goat Raw Milk and Cream Cheese Open
Infection with the tick-borne encephalitis virus (TBEV) can cause meningitis, meningoencephalitis and myelitis in humans. TBEV is an enveloped RNA virus of the family Flaviviridae , which is mostly transmitted via tick bites. However, tran…
View article: Full-Length Genomic RNA of Foot-and-Mouth Disease Virus Is Infectious for Cattle by Injection
Full-Length Genomic RNA of Foot-and-Mouth Disease Virus Is Infectious for Cattle by Injection Open
Safe sample transport is of great importance for infectious diseases diagnostics. Various treatments and buffers are used to inactivate pathogens in diagnostic samples. At the same time, adequate sample preservation, particularly of nuclei…
View article: The Experimental Infection of Goats with Small Ruminant Morbillivirus Originated from Barbary Sheep
The Experimental Infection of Goats with Small Ruminant Morbillivirus Originated from Barbary Sheep Open
Peste des Petits Ruminants (PPR) is a transboundary contagious disease in domestic small ruminants. Infections with the small ruminant morbillivirus (SRMV) were regularly found in wildlife, with unknown roles in PPR epidemiology. In order …
View article: Evidence for Different Virulence Determinants and Host Response after Infection of Turkeys and Chickens with Highly Pathogenic H7N1 Avian Influenza Virus
Evidence for Different Virulence Determinants and Host Response after Infection of Turkeys and Chickens with Highly Pathogenic H7N1 Avian Influenza Virus Open
Infection with HPAIV in chickens and turkeys, two closely related galliform species, results in severe disease and death. Although the presence of a polybasic cleavage site (pCS) in the hemagglutinin of AIV is a major virulence determinant…